LOCUS       pZMU-Dest          8681 bp    DNA     circular     17-FEB-2012
DEFINITION  mbSUS.
KEYWORDS    Split-Ubiquitin.
SOURCE      
  ORGANISM  
REFERENCE   
  AUTHORS   Christopher Grefen
FEATURES             Location/Qualifiers
     rep_origin      complement(6157..6671)
                     /label=f1\ORI
     rep_origin      2568..3768
                     /label=2µ\ORI
     CDS             complement(4549..5352)
                     /label=URA3
     rep_origin      8252..8532
                     /label=pBR322\OR
     CDS             6792..7802
                     /label=SpecR
     misc_feature    4404..4404
                     /label=end\of\URA3\gene
     promoter        40..396
                     /label=adh
     exon            2157..2180
                     /label=c-myc
     misc_recomb     424..548
                     /label=attR1
     CDS             1658..1963
                     /label=ccdB
     misc_recomb     complement(2004..2128)
                     /label=attR2
     CDS             657..1313
                     /label=CmR
BASE COUNT     2484 a      1876 c      1898 g      2423 t 
ORIGIN
        1 caacttcttt tctttttttt tcttttctct ctcccccgtt gttgtctcac catatccgca 
       61 atgacaaaaa aatgatggaa gacactaaag gaaaaaatta acgacaaaga cagcaccaac 
      121 agatgtcgtt gttccagagc tgatgagggg tatctcgaag cacacgaaac tttttccttc 
      181 cttcattcac gcacactact ctctaatgag caacggtata cggccttcct tccagttact 
      241 tgaatttgaa ataaaaaaaa gtttgctgtc ttgctatcaa gtataaatag acctgcaatt 
      301 attaatcttt tgtttcctcg tcattgttct cgttcccttt cttccttgtt tctttttctg 
      361 cacaatattt caagctatac caagcataca atcaactcca agctggatcc tctagacgag 
      421 ctcacaagtt tgtacaaaaa agctgaacga gaaacgtaaa atgatataaa tatcaatata 
      481 ttaaattaga ttttgcataa aaaacagact acataatact gtaaaacaca acatatccag 
      541 tcactatggc ggccgcatta ggcaccccag gctttacact ttatgcttcc ggctcgtata 
      601 atgtgtggat tttgagttag gatccgtcga gattttcagg agctaaggaa gctaaaatgg 
      661 agaaaaaaat cactggatat accaccgttg atatatccca atggcatcgt aaagaacatt 
      721 ttgaggcatt tcagtcagtt gctcaatgta cctataacca gaccgttcag ctggatatta 
      781 cggccttttt aaagaccgta aagaaaaata agcacaagtt ttatccggcc tttattcaca 
      841 ttcttgcccg cctgatgaat gctcatccgg aattccgtat ggcaatgaaa gacggtgagc 
      901 tggtgatatg ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa actgaaacgt 
      961 tttcatcgct ctggagtgaa taccacgacg atttccggca gtttctacac atatattcgc 
     1021 aagatgtggc gtgttacggt gaaaacctgg cctatttccc taaagggttt attgagaata 
     1081 tgtttttcgt ctcagccaat ccctgggtga gtttcaccag ttttgattta aacgtggcca 
     1141 atatggacaa cttcttcgcc cccgttttca ccatgggcaa atattatacg caaggcgaca 
     1201 aggtgctgat gccgctggcg attcaggttc atcatgccgt ttgtgatggc ttccatgtcg 
     1261 gcagaatgct taatgaatta caacagtact gcgatgagtg gcagggcggg gcgtaaacgc 
     1321 gtggatccgg cttactaaaa gccagataac agtatgcgta tttgcgcgct gatttttgcg 
     1381 gtataagaat atatactgat atgtataccc gaagtatgtc aaaaagaggt atgctatgaa 
     1441 gcagcgtatt acagtgacag ttgacagcga cagctatcag ttgctcaagg catatatgat 
     1501 gtcaatatct ccggtctggt aagcacaacc atgcagaatg aagcccgtcg tctgcgtgcc 
     1561 gaacgctgga aagcggaaaa tcaggaaggg atggctgagg tcgcccggtt tattgaaatg 
     1621 aacggctctt ttgctgacga gaacaggggc tggtgaaatg cagtttaagg tttacaccta 
     1681 taaaagagag agccgttatc gtctgtttgt ggatgtacag agtgatatta ttgacacgcc 
     1741 cgggcgacgg atggtgatcc ccctggccag tgcacgtctg ctgtcagata aagtctcccg 
     1801 tgaactttac ccggtggtgc atatcgggga tgaaagctgg cgcatgatga ccaccgatat 
     1861 ggccagtgtg ccggtctccg ttatcgggga agaagtggct gatctcagcc accgcgaaaa 
     1921 tgacatcaaa aacgccatta acctgatgtt ctggggaata taaatgtcag gctcccttat 
     1981 acacagccag tctgcaggtc gaccatagtg actggatatg ttgtgtttta cagtattatg 
     2041 tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt 
     2101 ctcgttcagc tttcttgtac aaagtggtga ctcgagccgc catcatggag gagcagaagc 
     2161 tgatctcaga ggaggacctg cactgaagct ttggacttct tcgccagagg tttggtcaag 
     2221 tctccaatca aggttgtcgg cttgtctacc ttgccagaaa tttacgaaaa gatggaaaag 
     2281 ggtcaaatcg ttggtagata cgttgttgac acttctaaat aagcgaattt cttatgattt 
     2341 atgattttta ttattaaata agttataaaa aaaataagtg tatacaaatt ttaaagtgac 
     2401 tcttaggttt taaaacgaaa attcttattc ttgagtaact ctttcctgta ggtcaggttg 
     2461 ctttctcagg tatagcatga ggtcgctctt attgaccaca cctctaccgg catgcgatat 
     2521 gatccaatat caaaggaaat gatagcattg aaggatgaga ctaatccaat tgaggagtgg 
     2581 cagcatatag aacagctaaa gggtagtgct gaaggaagca tacgataccc cgcatggaat 
     2641 gggataatat cacaggaggt actagactac ctttcatcct acataaatag acgcatataa 
     2701 gtacgcattt aagcataaac acgcactatg ccgttcttct catgtatata tatatacagg 
     2761 caacacgcag atataggtgc gacgtgaaca gtgagctgta tgtgcgcagc tcgcgttgca 
     2821 ttttcggaag cgctcgtttt cggaaacgct ttgaagttcc tattccgaag ttcctattct 
     2881 ctagctagaa agtataggaa cttcagagcg cttttgaaaa ccaaaagcgc tctgaagacg 
     2941 cactttcaaa aaaccaaaaa cgcaccggac tgtaacgagc tactaaaata ttgcgaatac 
     3001 cgcttccaca aacattgctc aaaagtatct ctttgctata tatctctgtg ctatatccct 
     3061 atataaccta cccatccacc tttcgctcct tgaacttgca tctaaactcg acctctacat 
     3121 tttttatgtt tatctctagt attactcttt agacaaaaaa attgtagtaa gaactattca 
     3181 tagagtgaat cgaaaacaat acgaaaatgt aaacatttcc tatacgtagt atatagagac 
     3241 aaaatagaag aaaccgttca taattttctg accaatgaag aatcatcaac gctatcactt 
     3301 tctgttcaca aagtatgcgc aatccacatc ggtatagaat ataatcgggg gatgccttta 
     3361 tcttgaaaaa atgcacccgc agcttcgcta gtaatcagta aacgcgggaa gtggagtcag 
     3421 gcttttttta tggaagagaa aatagacacc aaagtagcct tcttctaacc ttaacggacc 
     3481 tacagtgcaa aaagttatca agagactgca ttatagagcg cacaaaggag aaaaaaagta 
     3541 atctaagatg ctttgttaga aaaatagcgc tctcgggatg catttttgta gaacaaaaaa 
     3601 gaagtataga ttctttgttg gtaaaatagc gctctcgcgt tgcatttctg ttctgtaaaa 
     3661 atgcagctca gattctttgt ttgaaaaatt agcgctctcg cgttgcattt ttgttttaca 
     3721 aaaatgaagc acagattctt cgttggtaaa atagcgcttt cgcgttgcat ttctgttctg 
     3781 taaaaatgca gctcagattc tttgtttgaa aaattagcgc tctcgcgttg catttttgtt 
     3841 ctacaaaatg aagcacagat gcttcgttaa caaagatatg ctattgaagt gcaagatgga 
     3901 aacgcagaaa atgaaccggg gatgcgacgt gcaagattac ctatgcaata gatgcaatag 
     3961 tttctccagg aaccgaaata catacattgt cttccgtaaa gcgctagact atatattatt 
     4021 atacaggttc aaatatacta tctgtttcag ggaaaactcc caggttcgga tgttcaaaat 
     4081 tcaatgatgg gtaacaagta cgatcgtaaa tctgtaaaac agtttgtcgg atattaggct 
     4141 gtatctcctc aaagcgtatt cgaatatcat tgagaagctg catttttttt tttttttttt 
     4201 tttttttttt ttaatatata tttcaaggat ataccattgt aatgtctgcc cctaagaaga 
     4261 tcgtcgtttt gccaggtgac cacgttggtc aagaaatcac agccgaagcc attaaggttc 
     4321 ttaaagctat ttctgatgtt cgttccaatg tcaagttcga tttcgaaaat catttaattg 
     4381 gtggtgctgc tatcgcagat ccagatctgg ctttttcttt ccaatttttt ttttttcgtc 
     4441 attataaaaa tcattacgac cgagattccc gggtaataac tgatataatt aaattgaagc 
     4501 tctaatttgt gagtttagta tacatgcatt tacttataat acagtttttt agttttgctg 
     4561 gccgcatctt ctcaaatatg cttcccagcc tgcttttctg taacgttcac cctctacctt 
     4621 agcatccctt ccctttgcaa atagtcctct tccaacaata ataatgtcag atcctgtaga 
     4681 gaccacatca tccacggttc tatactgttg acccaatgcg tctcccttgt catctaaacc 
     4741 cacaccgggt gtcataatca accaatcgta accttcatct cttccaccca tgtctctttg 
     4801 agcaataaag ccgataacaa aatctttgtc gctcttcgca atgtcaacag tacccttagt 
     4861 atattctcca gtagataggg agcccttgca tgacaattct gctaacatca aaaggcctct 
     4921 aggttccttt gttacttctt ctgccgcctg cttcaaaccg ctaacaatac ctgggcccac 
     4981 cacaccgtgt gcattcgtaa tgtctgccca ttctgctatt ctgtatacac ccgcagagta 
     5041 ctgcaatttg actgtattac caatgtcagc aaattttctg tcttcgaaga gtaaaaaatt 
     5101 gtacttggcg gataatgcct ttagcggctt aactgtgccc tccatggaaa aatcagtcaa 
     5161 gatatccaca tgtgttttta gtaaacaaat tttgggacct aatgcttcaa ctaactccag 
     5221 taattccttg gtggtacgaa catccaatga agcacacaag tttgtttgct tttcgtgcat 
     5281 gatattaaat agcttggcag caacaggact aggatgagta gcagcacgtt ccttatatgt 
     5341 agctttcgac atgatttatc ttcgtttccg gtttttgttc tgtgcagttg ggttaagaat 
     5401 actgggcaat ttcatgtttc ttcaacacta catatgcgta tatataccaa tctaagtctg 
     5461 tgctccttcc ttcgttcttc cttctgttcg gagattaccg aatcaaaaaa atttcaagga 
     5521 aaccgaaatc aaaaaaaaga ataaaaaaaa aatgatgaat tgaaaagcca gatctgatca 
     5581 tctccgatga agcctccgtt atcccaggct ccttgggttt gttgccatct gcgtccttgg 
     5641 cctctttgcc agacaagaac accgcatttg gtttgtacga accatgccat ggttccgctc 
     5701 cagatttgcc aaagaataag gtcaacccta tcgccactat cttgtctgct gcaatgatgt 
     5761 tgaaattgtc attgaacttg cctgaagaag gtaaagccat tgaagatgca gttaaaaagg 
     5821 ttttggatgc aggtatcaga actggtgatt taggtggttc caacagtacc accgaagtcg 
     5881 gtgatgctgt cgccgaagaa gttaagaaaa tccttgctta aaaagattct ctttttttat 
     5941 gatatttgtc caaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa atgcagcgtc 
     6001 acatcggata ataatgatgg cagccattgt agaagtgcct tttgcatttc tagtctcttt 
     6061 ctcggtctag ctagttttac tacatcgcga agatagaatc ttagatcaca ctgcctttgc 
     6121 tgagctggat caatagagta acaaaagagt ggtaaggacc ccggttgata atcagaaaag 
     6181 ccccaaaaac aggaagattg tataagcaaa tatttaaatt gtaagcgtta atattttgtt 
     6241 aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg 
     6301 caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg 
     6361 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta 
     6421 tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg 
     6481 ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa 
     6541 gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct 
     6601 ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct 
     6661 acagggcgcg tcctcgttaa aggacaagga cctgagcgga agtgtatcgt acagtagacg 
     6721 gagtatacta gtaggcacga acccagtgga cataagcctg ttcggttcgt aagctgtaat 
     6781 gcaagtagcg tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta 
     6841 acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttggggta cagtctatgc 
     6901 ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca 
     6961 gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc atgggggaag 
     7021 cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc 
     7081 tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc 
     7141 cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc 
     7201 gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc 
     7261 gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta 
     7321 agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc 
     7381 cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg 
     7441 ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg 
     7501 aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc 
     7561 gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc 
     7621 cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca 
     7681 tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag 
     7741 atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta gtcggcaaat 
     7801 aatgtctagc tagaaattcg ttcaagccga cgccgcttcg cggcgcggct taactcaagc 
     7861 gttagatgca ctaagcacat aattgctcac agccaaacta tcaggtcaag tctgctttta 
     7921 ttatttttaa gcgtgcataa taagccctac acaaattggg agatatatca tgcatgacca 
     7981 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag 
     8041 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac 
     8101 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa 
     8161 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc 
     8221 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag 
     8281 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac 
     8341 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc 
     8401 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc 
     8461 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca 
     8521 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc 
     8581 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg 
     8641 ccagcaacgc ggccttttta cggttcctgg ccttttgcat g 
//
