LOCUS       pXNubA22-DEST        8516 bp    DNA     circular     13-NOV-2006
SOURCE      
  ORGANISM  
REFERENCE   
  AUTHORS   Christopher Grefen
FEATURES             Location/Qualifiers
     exon            1769..2074
                     /label=ccdB
     misc_feature    complement(2115..2239)
                     /label=attR2
     exon            2271..2372
                     /label=NubA
     misc_feature    2385..2474
                     /label=3HA
     rep_origin      2711..3882
                     /label=pUC\ORI
     exon            complement(3882..4742)
                     /label=AmpR
     rep_origin      4873..6220
                     /label=2µ\ORI
     exon            6757..7431
                     /label=TRP1
     misc_feature    368..492
                     /label=attR1
     exon            768..1427
                     /label=CmR
     promoter        1..357
                     /label=ADH
     rep_origin      7482..8146
                     /label=f1\ORI
BASE COUNT     2354 a      1831 c      1963 g      2368 t 
ORIGIN
        1 tgttgtctca ccatatccgc aatgacaaaa aaatgatgga agacactaaa ggaaaaaatt 
       61 aacgacaaag acagcaccaa cagatgtcgt tgttccagag ctgatgaggg gtatctcgaa 
      121 gcacacgaaa ctttttcctt ccttcattca cgcacactac tctctaatga gcaacggtat 
      181 acggccttcc ttccagttac ttgaatttga aataaaaaaa agtttgctgt cttgctatca 
      241 agtataaata gacctgcaat tattaatctt ttgtttcctc gtcattgttc tcgttccctt 
      301 tcttccttgt ttctttttct gcacaatatt tcaagctata ccaagcatac aatcaactca 
      361 gctgtgaaca agtttgtaca aaaaagctga acgagaaacg taaaatgata taaatatcaa 
      421 tatattaaat tagattttgc ataaaaaaca gactacataa tactgtaaaa cacaacatat 
      481 ccagtcacta tggcggccgc gggtgatgct gcaacttagc ggccgctaag ttggcagcat 
      541 cacccgacgc actttgcgcc gaataaatac ctgtgacgga agatcacttc gcagaataaa 
      601 taaatcctgg tgtccctgtt gataccggga agccctgggc caacttttgg cgaaaatgag 
      661 acgttgatcg gcacgcaaga ggttccaact ttcaccataa tgaaataaga tcactaccgg 
      721 gcgtattttt tgagttatcg agattttcag gagctaagga agctaaaatg gagaaaaaaa 
      781 tcactggata taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat 
      841 ttcagtcagt tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt 
      901 taaagaccgt aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc 
      961 gcctgatgaa tgctcatccg gaattccgta tggcaatgaa agacggtgag ctggtgatat 
     1021 gggatagtgt tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcatcgc 
     1081 tctggagtga ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg 
     1141 cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgtttttcg 
     1201 tctcagccaa tccctgggtg agtttcacca gttttgattt aaacgtggcc aatatggaca 
     1261 acttcttcgc ccccgttttc accatgggca aatattatac gcaaggcgac aaggtgctga 
     1321 tgccgctggc gattcaggtt catcatgccg tttgtgatgg cttccatgtc ggcagaatgc 
     1381 ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg ggcgtaaacg cgtggatccg 
     1441 gcttactaaa agccagataa cagtatgcgt atttgcgcgc tgatttttgc ggtataagaa 
     1501 tatatactga tatgtatacc cgaagtatgt caaaaagagg tatgctatga agcagcgtat 
     1561 tacagtgaca gttgacagcg acagctatca gttgctcaag gcatatatga tgtcaatatc 
     1621 tccggtctgg taagcacaac catgcagaat gaagcccgtc gtctgcgtgc cgaacgctgg 
     1681 aaagcggaaa atcaggaagg gatggctgag gtcgcccggt ttattgaaat gaacggctct 
     1741 tttgctgacg agaacagggg ctggtgaaat gcagtttaag gtttacacct ataaaagaga 
     1801 gagccgttat cgtctgtttg tggatgtaca gagtgatatt attgacacgc ccgggcgacg 
     1861 gatggtgatc cccctggcca gtgcacgtct gctgtcagat aaagtctccc gtgaacttta 
     1921 cccggtggtg catatcgggg atgaaagctg gcgcatgatg accaccgata tggccagtgt 
     1981 gccggtctcc gttatcgggg aagaagtggc tgatctcagc caccgcgaaa atgacatcaa 
     2041 aaacgccatt aacctgatgt tctggggaat ataaatgtca ggctccctta tacacagcca 
     2101 gtctgcaggt cgaccatagt gactggatat gttgtgtttt acagtattat gtagtctgtt 
     2161 ttttatgcaa aatctaattt aatatattga tatttatatc attttacgtt tctcgttcag 
     2221 ctttcttgta caaagtggtt ggtggtggcg gatccggtgg aggtggatca atgcagattt 
     2281 tcgtcaagac tttgaccggt aaaaccgcaa cgttggaagt tgaatcttcc gataccatcg 
     2341 acaacgttaa gtcgaaaatt caagacaagg aaggaatccc tggttaccca tacgatgttc 
     2401 ctgactatgc gggctatccc tatgacgtcc cggactatgc aggatcctat ccatatgacg 
     2461 ttccagatta cgctgctcag tgctgaggcg cgccacttct aaataagcga atttcttatg 
     2521 atttatgatt tttattatta aataagttat aaaaaaaata agtgtataca aattttaaag 
     2581 tgactcttag gttttaaaac gaaaattctt attcttgagt aactctttcc tgtaggtcag 
     2641 gttgctttct caggtatagt atgaggtcgc tcttattgac cacacctcta ccggcagcat 
     2701 gcaagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca 
     2761 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg 
     2821 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg 
     2881 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc 
     2941 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta 
     3001 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag 
     3061 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg 
     3121 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg 
     3181 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg 
     3241 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga 
     3301 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc 
     3361 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt 
     3421 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact 
     3481 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg 
     3541 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt 
     3601 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt 
     3661 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct 
     3721 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg 
     3781 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt 
     3841 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt 
     3901 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc 
     3961 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg 
     4021 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc 
     4081 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg 
     4141 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca 
     4201 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga 
     4261 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct 
     4321 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg 
     4381 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca 
     4441 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata 
     4501 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct 
     4561 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact 
     4621 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa 
     4681 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc 
     4741 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga 
     4801 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga 
     4861 aaagtgccac ctgaacgaag catctgtgct tcattttgta gaacaaaaat gcaacgcgag 
     4921 agcgctaatt tttcaaacaa agaatctgag ctgcattttt acagaacaga aatgcaacgc 
     4981 gaaagcgcta ttttaccaac gaagaatctg tgcttcattt ttgtaaaaca aaaatgcaac 
     5041 gcgagagcgc taatttttca aacaaagaat ctgagctgca tttttacaga acagaaatgc 
     5101 aacgcgagag cgctatttta ccaacaaaga atctatactt cttttttgtt ctacaaaaat 
     5161 gcatcccgag agcgctattt ttctaacaaa gcatcttaga ttactttttt tctcctttgt 
     5221 gcgctctata atgcagtctc ttgataactt tttgcactgt aggtccgtta aggttagaag 
     5281 aaggctactt tggtgtctat tttctcttcc ataaaaaaag cctgactcca cttcccgcgt 
     5341 ttactgatta ctagcgaagc tgcgggtgca ttttttcaag ataaaggcat ccccgattat 
     5401 attctatacc gatgtggatt gcgcatactt tgtgaacaga aagtgatagc gttgatgatt 
     5461 cttcattggt cagaaaatta tgaacggttt cttctatttt gtctctatat actacgtata 
     5521 ggaaatgttt acattttcgt attgttttcg attcactcta tgaatagttc ttactacaat 
     5581 ttttttgtct aaagagtaat actagagata aacataaaaa atgtagaggt cgagtttaga 
     5641 tgcaagttca aggagcgaaa ggtggatggg taggttatat agggatatag cacagagata 
     5701 tatagcaaag agatactttt gagcaatgtt tgtggaagcg gtattcgcaa tattttagta 
     5761 gctcgttaca gtccggtgcg tttttggttt tttgaaagtg cgtcttcaga gcgcttttgg 
     5821 ttttcaaaag cgctctgaag ttcctatact ttctagagaa taggaacttc ggaataggaa 
     5881 cttcaaagcg tttccgaaaa cgagcgcttc cgaaaatgca acgcgagctg cgcacataca 
     5941 gctcactgtt cacgtcgcac ctatatctgc gtgttgcctg tatatatata tacatgagaa 
     6001 gaacggcata gtgcgtgttt atgcttaaat gcgtacttat atgcgtctat ttatgtagga 
     6061 tgaaaggtag tctagtacct cctgtgatat tatcccattc catgcggggt atcgtatgct 
     6121 tccttcagca ctacccttta gctgttctat atgctgccac tcctcaattg gattagtctc 
     6181 atccttcaat gctatcattt cctttgatat tggatcatac taagaaacca ttattatcat 
     6241 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga 
     6301 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc 
     6361 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg 
     6421 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata gatcaacgac 
     6481 attactatat atataatata ggaagcattt aatagaacag catcgtaata tatgtgtact 
     6541 ttgcagttat gacgccagat ggcagtagtg gaagatattc tttattgaaa aatagcttgt 
     6601 caccttacgt acaatcttga tccggagctt ttcttttttt gccgattaag aattaattcg 
     6661 gtcgaaaaaa gaaaaggaga gggccaagag ggagggcatt ggtgactatt gagcacgtga 
     6721 gtatacgtga ttaagcacac aaaggcagct tggagtatgt ctgttattaa tttcacaggt 
     6781 agttctggtc cattggtgaa agtttgcggc ttgcagagca cagaggccgc agaatgtgct 
     6841 ctagattccg atgctgactt gctgggtatt atatgtgtgc ccaatagaaa gagaacaatt 
     6901 gacccggtta ttgcaaggaa aatttcaagt cttgtaaaag catataaaaa tagttcaggc 
     6961 actccgaaat acttggttgg cgtgtttcgt aatcaaccta aggaggatgt tttggctctg 
     7021 gtcaatgatt acggcattga tatcgtccaa ctgcatggag atgagtcgtg gcaagaatac 
     7081 caagagttcc tcggtttgcc agttattaaa agactcgtat ttccaaaaga ctgcaacata 
     7141 ctactcagtg cagcttcaca gaaacctcat tcgtttattc ccttgtttga ttcagaagca 
     7201 ggtgggacag gtgaactttt ggattggaac tcgatttctg actgggttgg aaggcaagag 
     7261 agccccgaaa gcttacattt tatgttagct ggtggactga cgccagaaaa tgttggtgat 
     7321 gcgcttagat taaatggcgt tattggtgtt gatgtaagcg gaggtgtgga gacaaatggt 
     7381 gtaaaagact ctaacaaaat agcaaatttc gtcaaaaatg ctaagaaata ggttattact 
     7441 gagtagtatt tatttaagta ttgtttgtgc acttgccgat ctatgcggtg tgaaataccg 
     7501 cacagatgcg taaggagaaa ataccgcatc aggaaattgt aagcgttaat attttgttaa 
     7561 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca 
     7621 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga 
     7681 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc 
     7741 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc 
     7801 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc 
     7861 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg 
     7921 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac 
     7981 agggcgcgtc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc 
     8041 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt 
     8101 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca agctttcgcg 
     8161 agctcgatat ccttttgttg tttccgggtg tacaatatgg acttcctctt ttctggcaac 
     8221 caaacccata catcgggatt cctataatac cttcgttggt ctccctaaca tgtaggtggc 
     8281 ggaggggaga tatacaatag aacagatacc agacaagaca taatgggcta aacaagacta 
     8341 caccaattac actgcctcat tgatggtggt acataacgaa ctaatactgt agccctagac 
     8401 ttgatagcca tcatcatatc gaagtttcac tacccttttt ccatttgcca tctattgaag 
     8461 taataatagg cgcatgcaac ttcttttctt ttttttttct tttctctctc ccccgt 
//
