LOCUS       pUC-R1R4         4398 bp    DNA     circular     19-JUL-2011
DEFINITION  2in1 vector conversion kit.
KEYWORDS    2in1.
SOURCE      
  ORGANISM  
REFERENCE   
  AUTHORS   Grefen,C., and Blatt,M.R.
  TITLE     A 2in1 cloning system enables ratiometric bimolecular fluorescence complementation (rBiFC).
  JOURNAL   Biotechniques. 2012 Oct 2;53(5):311-14.
   PUBMED   23066669
FEATURES             Location/Qualifiers
     rep_origin      complement(2564..3178)
                     /label=pMDB1
     CDS             complement(3338..4198)
                     /label=AmpR
     misc_recomb     complement(1994..2118)
                     /label=attR4
     CDS             647..1306
                     /label=CmR
     CDS             1648..1950
                     /label=ccdB
     misc_recomb     414..538
                     /label=attR1
BASE COUNT     1155 a      1021 c      1081 g      1141 t 
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cactagtcct agaacaagtt 
      421 tgtacaaaaa agctgaacga gaaacgtaaa atgatataaa tatcaatata ttaaattaga 
      481 ttttgcataa aaaacagact acataatact gtaaaacaca acatatccag tcactatggc 
      541 ggccgcatta ggcaccccag gctttacact ttatgcttcc ggctcgtata atgtgtggat 
      601 tttgagttag gatccgtcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat 
      661 cactggatat accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt 
      721 tcagtcagtt gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt 
      781 aaagaccgta aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg 
      841 cctgatgaat gctcatccgg aattccgtat ggcaatgaaa gacggtgagc tggtgatatg 
      901 ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct 
      961 ctggagtgaa taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc 
     1021 gtgttacggt gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt 
     1081 ctcagccaat ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa 
     1141 cttcttcgcc cccgttttca ccatgggcaa atattatacg caaggcgaca aggtgctgat 
     1201 gccgctggcg attcaggttc atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct 
     1261 taatgaatta caacagtact gcgatgagtg gcagggcggg gcgtaaagat ctggatccgg 
     1321 cttactaaaa gccagataac agtatgcgta tttgcgcgct gatttttgcg gtataagaat 
     1381 atatactgat atgtataccc gaagtatgtc aaaaagaggt atgctatgaa gcagcgtatt 
     1441 acagtgacag ttgacagcga cagctatcag ttgctcaagg catatatgat gtcaatatct 
     1501 ccggtctggt aagcacaacc atgcagaatg aagcccgtcg tctgcgtgcc gaacgctgga 
     1561 aagcggaaaa tcaggaaggg atggctgagg tcgcccggtt tattgaaatg aacggctctt 
     1621 ttgctgacga gaacaggggc tggtgaaatg cagtttaagg tttacaccta taaaagagag 
     1681 agccgttatc gtctgtttgt ggatgtacag agtgatatta ttgacacgcc cgggcgacgg 
     1741 atggtgatcc ccctggccag tgcacgtctg ctgtcagata aagtctcccg tgaactttac 
     1801 ccggtggtgc atatcgggga tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg 
     1861 ccggtctccg ttatcgggga agaagtggct gatctcagcc accgcgaaaa tgacatcaaa 
     1921 aacgccatta acctgatgtt ctggggaata taaatgtcag gctcccttat acacagccag 
     1981 tctgcaggtc gaccatagtg actggatatg ttgtgtttta cagtattatg tagtctgttt 
     2041 tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt ctcgttcaac 
     2101 ttttctatac aaagttggtc aattgttata aatcggatcc cgggcccgtc gactgcagaa 
     2161 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc 
     2221 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct 
     2281 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc 
     2341 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt 
     2401 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag 
     2461 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca 
     2521 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt 
     2581 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc 
     2641 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct 
     2701 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg 
     2761 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca 
     2821 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact 
     2881 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta 
     2941 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta 
     3001 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct 
     3061 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt 
     3121 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga 
     3181 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca 
     3241 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat 
     3301 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg 
     3361 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt 
     3421 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag 
     3481 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc 
     3541 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag 
     3601 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca 
     3661 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa 
     3721 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga 
     3781 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata 
     3841 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca 
     3901 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg 
     3961 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg 
     4021 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg 
     4081 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag 
     4141 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac 
     4201 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca 
     4261 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag 
     4321 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta 
     4381 tcacgaggcc ctttcgtc 
//
