LOCUS       pUBN-RFP-DEST       11450 bp    DNA     circular     30-SEP-2010
SOURCE      
  ORGANISM  
REFERENCE   1  (bases 1 to 10818)
  AUTHORS   Grefen,C., Donald,N., Hashimoto,K., Kudla,J., Schumacher,K. and
            Blatt,M.R.
  TITLE     A ubiquitin-10 promoter-based vector set for fluorescent protein
            tagging facilitates temporal stability and native protein
            distribution in transient and stable expression studies
  JOURNAL   Plant J. 64 (2), 355-365 (2010)
   PUBMED   20735773
FEATURES             Location/Qualifiers
     misc_feature    complement(4493..4825)
                     /label=LB
     misc_feature    11010..11158
                     /label=RB
     terminator      3081..3289
                     /label=T35S
     CDS             3339..4489
                     /label=BarR
     promoter        1..639
                     /label=pUBQ10
     CDS             4889..5899
                     /label=SpecR
     misc_recomb     1353..1477
                     /label=attR1
     CDS             2586..2891
                     /label=ccdB
     misc_recomb     complement(2932..3056)
                     /label=attR2
     CDS             1586..2263
                     /label=CmR
     exon            666..1340
                     /label=RFP
     rep_origin      6350..7030
                     /label=pBR322
     rep_origin      7440..10033
                     /label=pVS1
BASE COUNT     2570 a      3079 c      3031 g      2770 t 
ORIGIN
        1 tacccgacga gtcagtaata aacggcgtca aagtggttgc agccggcaca cacgagtcgt 
       61 gtttatcaac tcaaagcaca aatacttttc ctcaacctaa aaataaggca attagccaaa 
      121 aacaactttg cgtgtaaaca acgctcaata cacgtgtcat tttattatta gctattgctt 
      181 caccgcctta gctttctcgt gacctagtcg tcctcgtctt ttcttcttct tcttctataa 
      241 aacaataccc aaagagctct tcttcttcac aattcagatt tcaatttctc aaaatcttaa 
      301 aaactttctc tcaattctct ctaccgtgat caaggtaaat ttctgtgttc cttattctct 
      361 caaaatcttc gattttgttt tcgttcgatc ccaatttcgt atatgttctt tggtttagat 
      421 tctgttaatc ttagatcgaa gacgattttc tgggtttgat cgttagatat catcttaatt 
      481 ctcgattagg gtttcataga tatcatccga tttgttcaaa taatttgagt tttgtcgaat 
      541 aattactctt cgatttgtga tttctatcta gatctggtgt tagtttctag tttgtgcgat 
      601 cgaatttgta gattaatctg agtttttctg attaacactc gagtgcggga tcctctagaa 
      661 ctagtatggc ctcctccgag gacgtcatca aggagttcat gcgcttcaag gtgcgcatgg 
      721 agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg 
      781 agggcaccca gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg 
      841 acatcctgtc ccctcagttc cagtacggct ccaaggccta cgtgaagcac cccgccgaca 
      901 tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact 
      961 tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca 
     1021 tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga 
     1081 agaccatggg ctgggaggcc tccaccgagc ggatgtaccc cgaggacggc gccctgaagg 
     1141 gcgagatcaa gatgaggctg aagctgaagg acggcggcca ctacgacgcc gaggtcaaga 
     1201 ccacctacat ggccaagaag cccgtgcagc tgcccggcgc ctacaagacc gacatcaagc 
     1261 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgag cgcgccgagg 
     1321 gccgccactc caccggcgcc caattgttaa tcacaagttt gtacaaaaaa gctgaacgag 
     1381 aaacgtaaaa tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta 
     1441 cataatactg taaaacacaa catatccagt cactatggcg gccgcattag gcaccccagg 
     1501 ctttacactt tatgcttccg gctcgtataa tgtgtggatt ttgagttagg atccgtcgag 
     1561 attttcagga gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga 
     1621 tatatcccaa tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac 
     1681 ctataaccag accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa 
     1741 gcacaagttt tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga 
     1801 attccgtatg gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta 
     1861 caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga 
     1921 tttccggcag tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc 
     1981 ctatttccct aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag 
     2041 tttcaccagt tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac 
     2101 catgggcaaa tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca 
     2161 tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg 
     2221 cgatgagtgg caggcggggc gtaatctaga ggatccggct tactaaaagc cagataacag 
     2281 tatgcgtatt tgcgcgctga tttttgcggt ataagaatat atactgatat gtatacccga 
     2341 agtatgtcaa aaagaggtat gctatgaagc agcgtattac agtgacagtt gacagcgaca 
     2401 gctatcagtt gctcaaggca tatatgatgt caatatctcc ggtctggtaa gcacaaccat 
     2461 gcagaatgaa gcccgtcgtc tgcgtgccga acgctggaaa gcggaaaatc aggaagggat 
     2521 ggctgaggtc gcccggttta ttgaaatgaa cggctctttt gctgacgaga acaggggctg 
     2581 gtgaaatgca gtttaaggtt tacacctata aaagagagag ccgttatcgt ctgtttgtgg 
     2641 atgtacagag tgatattatt gacacgcccg ggcgacggat ggtgatcccc ctggccagtg 
     2701 cacgtctgct gtcagataaa gtctcccgtg aactttaccc ggtggtgcat atcggggatg 
     2761 aaagctggcg catgatgacc accgatatgg ccagtgtgcc ggtctccgtt atcggggaag 
     2821 aagtggctga tctcagccac cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct 
     2881 ggggaatata aatgtcaggc tcccttatac acagccagtc tgcaggtcga ccatagtgac 
     2941 tggatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat ctaatttaat 
     3001 atattgatat ttatatcatt ttacgtttct cgttcagctt tcttgtacaa agtggtgatt 
     3061 aagcggccat gctagagtcc gcaaaaatca ccagtctctc tctacaaatc tatctctctc 
     3121 tatttttctc cagaataatg tgtgagtagt tcccagataa gggaattagg gttcttatag 
     3181 ggtttcgctc atgtgttgag catataagaa acccttagta tgtatttgta tttgtaaaat 
     3241 acttctatca ataaaatttc taattcctaa aaccaaaatc cagtgacctg caggcatgcg 
     3301 acgtcgggcc ctctagagga tccccgggta ccgcgaatta tcgatcatga gcggagaatt 
     3361 aagggagtca cgttatgacc cccgccgatg acgcgggaca agccgtttta cgtttggaac 
     3421 tgacagaacc gcaacgttga aggagccact gagccgcggg tttctggagt ttaatgagct 
     3481 aagcacatac gtcagaaacc attattgcgc gttcaaaagt cgcctaaggt cactatcagc 
     3541 tagcaaatat ttcttgtcaa aaatgctcca ctgacgttcc ataaattccc ctcggtatcc 
     3601 aattagagtc tcatattcac tctcaactcg atcgagggga tctaccatga gcccagaacg 
     3661 acgcccggcc gacatccgcc gtgccaccga ggcggacatg ccggcggtct gcaccatcgt 
     3721 caaccactac atcgagacaa gcacggtcaa cttccgtacc gagccgcagg aaccgcagga 
     3781 gtggacggac gacctcgtcc gtctgcggga gcgctatccc tggctcgtcg ccgaggtgga 
     3841 cggcgaggtc gccggcatcg cctacgcggg tccctggaag gcacgcaacg cctacgactg 
     3901 gacggccgag tcgaccgtgt acgtctcccc ccgccaccag cggacgggac tgggctccac 
     3961 gctctacacc cacctgctga agtccctgga ggcacagggc ttcaagagcg tggtcgctgt 
     4021 catcgggctg cccaacgacc cgagcgtgcg catgcacgag gcgctcggat atgccccccg 
     4081 cggcatgctg cgggcggccg gcttcaagca cgggaactgg catgacgtgg gtttctggca 
     4141 gctggacttc agcctgccgg tgccgccccg tccggtcctg cccgtcaccg aaatctgatg 
     4201 acccctagag tcaagcagat cgttcaaaca tttggcaata aagtttctta agattgaatc 
     4261 ctgttgccgg tcttgcgatg attatcatat aatttctgtt gaattacgtt aagcatgtaa 
     4321 taattaacat gtaatgcatg acgttattta tgagatgggt ttttatgatt agagtcccgc 
     4381 aattatacat ttaatacgcg atagaaaaca aaatatagcg cgcaaactag gataaattat 
     4441 cgcgcgcggt gtcatctatg ttactagatc gaccggcatg caagctgata attcaattcg 
     4501 gcgttaattc agtacattaa aaacgtccgc aatgtgttat taagttgtct aagcgtcaat 
     4561 ttgtttacac cacaatatat cctgccacca gccagccaac agctccccga ccggcagctc 
     4621 ggcacaaaat caccactcga tacaggcagc ccatcagtcc gggacggcgt cagcgggaga 
     4681 gccgttgtaa ggcggcagac tttgctcatg ttaccgatgc tattcggaag aacggcaact 
     4741 aagctgccgg gtttgaaaca cggatgatct cgcggagggt agcatgttga ttgtaacgat 
     4801 gacagagcgt tgctgcctgt gatcaattcg ggcacgaacc cagtggacat aagcctgttc 
     4861 ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac 
     4921 cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt 
     4981 tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg 
     5041 tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt 
     5101 aaacatcatg ggggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg 
     5161 cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt 
     5221 ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct 
     5281 tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg 
     5341 agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc 
     5401 gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct 
     5461 tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc 
     5521 aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc 
     5581 tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga 
     5641 ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt 
     5701 aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc 
     5761 ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg 
     5821 cttggcctcg cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac 
     5881 caaggtagtc ggcaaataat gtctagctag aaattcgttc aagccgacgc cgcttcgcgg 
     5941 cgcggcttaa ctcaagtcgt tagatgcact aagcacataa ttgctcacag ccaaactatc 
     6001 aggtcaagtc tgcttttatt atttttaagc gtgcataata agccctacac aaattgggag 
     6061 atatatcatg catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc 
     6121 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct 
     6181 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa 
     6241 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag 
     6301 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc 
     6361 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg 
     6421 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca 
     6481 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat 
     6541 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg 
     6601 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc 
     6661 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc 
     6721 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc 
     6781 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg 
     6841 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga 
     6901 gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt 
     6961 cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt 
     7021 atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc 
     7081 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac 
     7141 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca 
     7201 gggtgccttg atgtgggcgc cggcggtcga gtggcgacgg cgcggcttgt ccgcgccctg 
     7261 gtagattgcc tggccctagg ccagccattt ttgagcggcc agcggccgcg ataggccgac 
     7321 gcgaagcggc ggggcgtagg gagcgcagcg accgaagggt aggcgctttt tgcagctctt 
     7381 cggctgtgcg ctggccagac agttatgcac aggccaggcg ggttttaaga gttttaataa 
     7441 gttttaaaga gttttaggcg gaaaaatcgc cttttttctc ttttatatca gtcacttaca 
     7501 tgtgtgaccg gttcccaatg tacggctttg ggttcccaat gtacgggttc cggttcccaa 
     7561 tgtacggctt tgggttccca atgtacgtgc tatccacagg aaagagacct tttcgacctt 
     7621 tttcccctgc tagggcaatt tgccctagca tctgctccgt acattaggaa ccggcggatg 
     7681 cttcgccctc gatcaggttg cggtagcgca tgactaggat cgggccagcc tgccccgcct 
     7741 cctccttcaa atcgtactcc ggcaggtcat ttgacccgat cagcttgcgc acggtgaaac 
     7801 agaacttctt gaactctccg gcgctgccac tgcgttcgta gatcgtcttg aacaaccatc 
     7861 tggcttctgc cttgcctgcg gcgcggcgtg ccaggcggta gagaaaacgg ccgatgccgg 
     7921 gatcgatcaa aaagtaatcg gggtgaaccg tcagcacgtc cgggttcttg ccttctgtga 
     7981 tctcgcggta catccaatca gctagctcga tctcgatgta ctccggccgc ccggtttcgc 
     8041 tctttacgat cttgtagcgg ctaatcaagg cttcaccctc ggataccgtc accaggcggc 
     8101 cgttcttggc cttcttcgta cgctgcatgg caacgtgcgt ggtgtttaac cgaatgcagg 
     8161 tttctaccag gtcgtctttc tgctttccgc catcggctcg ccggcagaac ttgagtacgt 
     8221 ccgcaacgtg tggacggaac acgcggccgg gcttgtctcc cttcccttcc cggtatcggt 
     8281 tcatggattc ggttagatgg gaaaccgcca tcagtaccag gtcgtaatcc cacacactgg 
     8341 ccatgccggc cggccctgcg gaaacctcta cgtgcccgtc tggaagctcg tagcggatca 
     8401 cctcgccagc tcgtcggtca cgcttcgaca gacggaaaac ggccacgtcc atgatgctgc 
     8461 gactatcgcg ggtgcccacg tcatagagca tcggaacgaa aaaatctggt tgctcgtcgc 
     8521 ccttgggcgg cttcctaatc gacggcgcac cggctgccgg cggttgccgg gattctttgc 
     8581 ggattcgatc agcggccgct tgccacgatt caccggggcg tgcttctgcc tcgatgcgtt 
     8641 gccgctgggc ggcctgcgcg gccttcaact tctccaccag gtcatcaccc agcgccgcgc 
     8701 cgatttgtac cgggccggat ggtttgcgac cgtcacgccg attcctcggg cttgggggtt 
     8761 ccagtgccat tgcagggccg gcagacaacc cagccgctta cgcctggcca accgcccgtt 
     8821 cctccacaca tggggcattc cacggcgtcg gtgcctggtt gttcttgatt ttccatgccg 
     8881 cctcctttag ccgctaaaat tcatctactc atttattcat ttgctcattt actctggtag 
     8941 ctgcgcgatg tattcagata gcagctcggt aatggtcttg ccttggcgta ccgcgtacat 
     9001 cttcagcttg gtgtgatcct ccgccggcaa ctgaaagttg acccgcttca tggctggcgt 
     9061 gtctgccagg ctggccaacg ttgcagcctt gctgctgcgt gcgctcggac ggccggcact 
     9121 tagcgtgttt gtgcttttgc tcattttctc tttacctcat taactcaaat gagttttgat 
     9181 ttaatttcag cggccagcgc ctggacctcg cgggcagcgt cgccctcggg ttctgattca 
     9241 agaacggttg tgccggcggc ggcagtgcct gggtagctca cgcgctgcgt gatacgggac 
     9301 tcaagaatgg gcagctcgta cccggccagc gcctcggcaa cctcaccgcc gatgcgcgtg 
     9361 cctttgatcg cccgcgacac gacaaaggcc gcttgtagcc ttccatccgt gacctcaatg 
     9421 cgctgcttaa ccagctccac caggtcggcg gtggcccata tgtcgtaagg gcttggctgc 
     9481 accggaatca gcacgaagtc ggctgccttg atcgcggaca cagccaagtc cgccgcctgg 
     9541 ggcgctccgt cgatcactac gaagtcgcgc cggccgatgg ccttcacgtc gcggtcaatc 
     9601 gtcgggcggt cgatgccgac aacggttagc ggttgatctt cccgcacggc cgcccaatcg 
     9661 cgggcactgc cctggggatc ggaatcgact aacagaacat cggccccggc gagttgcagg 
     9721 gcgcgggcta gatgggttgc gatggtcgtc ttgcctgacc cgcctttctg gttaagtaca 
     9781 gcgataacct tcatgcgttc cccttgcgta tttgtttatt tactcatcgc atcatatacg 
     9841 cagcgaccgc atgacgcaag ctgttttact caaatacaca tcaccttttt agacggcggc 
     9901 gctcggtttc ttcagcggcc aagctggccg gccaggccgc cagcttggca tcagacaaac 
     9961 cggccaggat ttcatgcagc cgcacggttg agacgtgcgc gggcggctcg aacacgtacc 
    10021 cggccgcgat catctccgcc tcgatctctt cggtaatgaa aaacggttcg tcctggccgt 
    10081 cctggtgcgg tttcatgctt gttcctcttg gcgttcattc tcggcggccg ccagggcgtc 
    10141 ggcctcggtc aatgcgtcct cacggaaggc accgcgccgc ctggcctcgg tgggcgtcac 
    10201 ttcctcgctg cgctcaagtg cgcggtacag ggtcgagcga tgcacgccaa gcagtgcagc 
    10261 cgcctctttc acggtgcggc cttcctggtc gatcagctcg cgggcgtgcg cgatctgtgc 
    10321 cggggtgagg gtagggcggg ggccaaactt cacgcctcgg gccttggcgg cctcgcgccc 
    10381 gctccgggtg cggtcgatga ttagggaacg ctcgaactcg gcaatgccgg cgaacacggt 
    10441 caacaccatg cggccggccg gcgtggtggt gtcggcccac ggctctgcca ggctacgcag 
    10501 gcccgcgccg gcctcctgga tgcgctcggc aatgtccagt aggtcgcggg tgctgcgggc 
    10561 caggcggtct agcctggtca ctgtcacaac gtcgccaggg cgtaggtggt caagcatcct 
    10621 ggccagctcc gggcggtcgc gcctggtgcc ggtgatcttc tcggaaaaca gcttggtgca 
    10681 gccggccgcg tgcagttcgg cccgttggtt ggtcaagtcc tggtcgtcgg tgctgacgcg 
    10741 ggcatagccc agcaggccag cggcggcgct cttgttcatg gcgtaatgtc tccggttcta 
    10801 gtcgcaagta ttctacttta tgcgactaaa acacgcgaca agaaaacgcc aggaaaaggg 
    10861 cagggcggca gcctgtcgcg taacttagga cttgtgcgac atgtcgtttt cagaagacgg 
    10921 ctgcactgaa cgtcagaagc cgactgcact atagcagcgg aggggttgga tcaaagtact 
    10981 ttaaagtact ttaaagtact ttaaagtact ttgatcccga ggggaaccct gtggttggca 
    11041 tgcacataca aatggacgaa cggataaacc ttttcacgcc cttttaaata tccgttattc 
    11101 taataaacgc tcttttctct taggtttacc cgccaatata tcctgtcaaa cactgatagt 
    11161 ttaaactgaa ggcgggaaac gacaatctga tccaagctca agctgctcta gccaatacgc 
    11221 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc 
    11281 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca 
    11341 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa 
    11401 caatttcaca caggaaacag ctatgaccat gattacgaat tcgagctcgg 
//
