LOCUS       pUBN-nYFP-Dest       11851 bp    DNA     circular     28-AUG-2015
SOURCE      
  ORGANISM  
REFERENCE   1  (bases 1 to 10818)
  AUTHORS   Grefen,C., Donald,N., Hashimoto,K., Kudla,J., Schumacher,K. and
            Blatt,M.R.
  TITLE     A ubiquitin-10 promoter-based vector set for fluorescent protein
            tagging facilitates temporal stability and native protein
            distribution in transient and stable expression studies
  JOURNAL   Plant J. 64 (2), 355-365 (2010)
   PUBMED   20735773
FEATURES             Location/Qualifiers
     misc_feature    complement(4894..5226)
                     /label=LB
     misc_feature    11411..11559
                     /label=RB
     terminator      2871..3079
                     /label=T35S
     promoter        1..639
                     /label=pUBQ10
     CDS             5290..6300
                     /label=SpecR
     misc_recomb     1143..1267
                     /label=attR1
     CDS             2376..2681
                     /label=ccdB
     misc_recomb     complement(2722..2846)
                     /label=attR2
     CDS             1376..2053
                     /label=CmR
     exon            666..1130
                     /label=nYFP
     rep_origin      6751..7431
                     /label=pMB1
     rep_origin      7841..10434
                     /label=pVS1
     CDS             3101..4890
                     /label=HygR
ORIGIN
        1 tacccgacga gtcagtaata aacggcgtca aagtggttgc agccggcaca cacgagtcgt 
       61 gtttatcaac tcaaagcaca aatacttttc ctcaacctaa aaataaggca attagccaaa 
      121 aacaactttg cgtgtaaaca acgctcaata cacgtgtcat tttattatta gctattgctt 
      181 caccgcctta gctttctcgt gacctagtcg tcctcgtctt ttcttcttct tcttctataa 
      241 aacaataccc aaagagctct tcttcttcac aattcagatt tcaatttctc aaaatcttaa 
      301 aaactttctc tcaattctct ctaccgtgat caaggtaaat ttctgtgttc cttattctct 
      361 caaaatcttc gattttgttt tcgttcgatc ccaatttcgt atatgttctt tggtttagat 
      421 tctgttaatc ttagatcgaa gacgattttc tgggtttgat cgttagatat catcttaatt 
      481 ctcgattagg gtttcataga tatcatccga tttgttcaaa taatttgagt tttgtcgaat 
      541 aattactctt cgatttgtga tttctatcta gatctggtgt tagtttctag tttgtgcgat 
      601 cgaatttgta gattaatctg agtttttctg attaacactc gagtgcggga tcctctagaa 
      661 ctagtatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc 
      721 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca 
      781 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc 
      841 ccaccctcgt gaccaccttc ggctacggcc tgcagtgctt cgcccgctac cccgaccaca 
      901 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca 
      961 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca 
     1021 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg 
     1081 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc caattgttaa 
     1141 tcacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa tgatataaat atcaatatat 
     1201 taaattagat tttgcataaa aaacagacta cataatactg taaaacacaa catatccagt 
     1261 cactatggcg gccgcattag gcaccccagg ctttacactt tatgcttccg gctcgtataa 
     1321 tgtgtggatt ttgagttagg atccgtcgag attttcagga gctaaggaag ctaaaatgga 
     1381 gaaaaaaatc actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt 
     1441 tgaggcattt cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac 
     1501 ggccttttta aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat 
     1561 tcttgcccgc ctgatgaatg ctcatccgga attccgtatg gcaatgaaag acggtgagct 
     1621 ggtgatatgg gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt 
     1681 ttcatcgctc tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca 
     1741 agatgtggcg tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat 
     1801 gtttttcgtc tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa 
     1861 tatggacaac ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa 
     1921 ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg 
     1981 cagaatgctt aatgaattac aacagtactg cgatgagtgg caggcggggc gtaatctaga 
     2041 ggatccggct tactaaaagc cagataacag tatgcgtatt tgcgcgctga tttttgcggt 
     2101 ataagaatat atactgatat gtatacccga agtatgtcaa aaagaggtat gctatgaagc 
     2161 agcgtattac agtgacagtt gacagcgaca gctatcagtt gctcaaggca tatatgatgt 
     2221 caatatctcc ggtctggtaa gcacaaccat gcagaatgaa gcccgtcgtc tgcgtgccga 
     2281 acgctggaaa gcggaaaatc aggaagggat ggctgaggtc gcccggttta ttgaaatgaa 
     2341 cggctctttt gctgacgaga acaggggctg gtgaaatgca gtttaaggtt tacacctata 
     2401 aaagagagag ccgttatcgt ctgtttgtgg atgtacagag tgatattatt gacacgcccg 
     2461 ggcgacggat ggtgatcccc ctggccagtg cacgtctgct gtcagataaa gtctcccgtg 
     2521 aactttaccc ggtggtgcat atcggggatg aaagctggcg catgatgacc accgatatgg 
     2581 ccagtgtgcc ggtctccgtt atcggggaag aagtggctga tctcagccac cgcgaaaatg 
     2641 acatcaaaaa cgccattaac ctgatgttct ggggaatata aatgtcaggc tcccttatac 
     2701 acagccagtc tgcaggtcga ccatagtgac tggatatgtt gtgttttaca gtattatgta 
     2761 gtctgttttt tatgcaaaat ctaatttaat atattgatat ttatatcatt ttacgtttct 
     2821 cgttcagctt tcttgtacaa agtggtgatt aagcggccat gctagagtcc gcaaaaatca 
     2881 ccagtctctc tctacaaatc tatctctctc tatttttctc cagaataatg tgtgagtagt 
     2941 tcccagataa gggaattagg gttcttatag ggtttcgctc atgtgttgag catataagaa 
     3001 acccttagta tgtatttgta tttgtaaaat acttctatca ataaaatttc taattcctaa 
     3061 aaccaaaatc cagtgacctg caggcatgcg acgtcgggcc atcatacatg agaattaagg 
     3121 gagtcacgtt atgaccccgc cgatgacgcg ggacaagccg ttttacgttt ggaactgaca 
     3181 gaaccgcaac gttgaaggag ccactgagcc gcgggtttct ggagtttaat gagctaagca 
     3241 catacgtcag aaaccattat tgcgcgttca aaagtcgcct aaggtcacta tcagctagca 
     3301 aatatttctt gtcaaaaatg ctccactgac gttccataaa ttcccctcgg tatccaatta 
     3361 gagtctcata ttcactctca actcgaatcc cccctatgaa aaagcctgaa ctcaccgcga 
     3421 cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg atgcagctct 
     3481 cggagggcga agaatctcgt gctttcagct tcgatgtagg agggcgtgga tatgtcctgc 
     3541 gggtaaatag ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg cactttgcat 
     3601 cggccgcgct cccgattccg gaagtgcttg acattgggga gttcagcgag agcctgacct 
     3661 attgcatctc ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa accgaactgc 
     3721 ccgctgttct tcagccggtc gcggaggcta tggatgctat cgctgcggcc gatcttagcc 
     3781 agacgagcgg gttcggccca ttcggaccgc aaggaatcgg tcaatacact acatggcgtg 
     3841 atttcatatg cgcgattgct gatccccatg tgtatcactg gcaaactgtg atggacgaca 
     3901 ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat gctttgggcc gaggactgcc 
     3961 ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa caatgtcctg acggacaatg 
     4021 gccgcataac agcggtcatt gactggagcg aggcgatgtt cggggattcc caatacgagg 
     4081 tcgccaacat cttcttctgg aggccgtggt tggcttgtat ggagcagcag acgcgctact 
     4141 tcgagcggag gcatccggag cttgcaggat cgccaaggct ccgggcgtat atgctccgca 
     4201 ttggtcttga ccaactctat cagagcttgg ttgacggcaa tttcgatgat gcagcttggg 
     4261 cgcagggtcg atgcgacgca atcgtccgat ccggagccgg gactgtcggg cgtacacaaa 
     4321 tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt agaagtactc gccgatagtg 
     4381 gaaaccgacg ccccagcact cgtccgaggg caaaggaata gagtagatgc cgaccgaaca 
     4441 agagctgatt tcgagaacgc ctcagccagc aactcgcgcg agcctagcaa ggcaaatgcg 
     4501 agagaacggc cttacgcttg gtggcacagt tctcgtccac agttcgctaa gctcgctcgg 
     4561 ctgggtcgcg ggagggccgg tcgcagtgat tcaggaatta attccctaga gtcaagcaga 
     4621 tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg gtcttgcgat 
     4681 gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca tgtaatgcat 
     4741 gacgttattt atgagatggg tttttatgat tagagtcccg caattataca tttaatacgc 
     4801 gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg tgtcatctat 
     4861 gttactagat cgaccggcat gcaagctgat aattcaattc ggcgttaatt cagtacatta 
     4921 aaaacgtccg caatgtgtta ttaagttgtc taagcgtcaa tttgtttaca ccacaatata 
     4981 tcctgccacc agccagccaa cagctccccg accggcagct cggcacaaaa tcaccactcg 
     5041 atacaggcag cccatcagtc cgggacggcg tcagcgggag agccgttgta aggcggcaga 
     5101 ctttgctcat gttaccgatg ctattcggaa gaacggcaac taagctgccg ggtttgaaac 
     5161 acggatgatc tcgcggaggg tagcatgttg attgtaacga tgacagagcg ttgctgcctg 
     5221 tgatcaattc gggcacgaac ccagtggaca taagcctgtt cggttcgtaa gctgtaatgc 
     5281 aagtagcgta tgcgctcacg caactggtcc agaaccttga ccgaacgcag cggtggtaac 
     5341 ggcgcagtgg cggttttcat ggcttgttat gactgttttt ttggggtaca gtctatgcct 
     5401 cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc 
     5461 aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt taaacatcat gggggaagcg 
     5521 gtgatcgccg aagtatcgac tcaactatca gaggtagttg gcgtcatcga gcgccatctc 
     5581 gaaccgacgt tgctggccgt acatttgtac ggctccgcag tggatggcgg cctgaagcca 
     5641 cacagtgata ttgatttgct ggttacggtg accgtaaggc ttgatgaaac aacgcggcga 
     5701 gctttgatca acgacctttt ggaaacttcg gcttcccctg gagagagcga gattctccgc 
     5761 gctgtagaag tcaccattgt tgtgcacgac gacatcattc cgtggcgtta tccagctaag 
     5821 cgcgaactgc aatttggaga atggcagcgc aatgacattc ttgcaggtat cttcgagcca 
     5881 gccacgatcg acattgatct ggctatcttg ctgacaaaag caagagaaca tagcgttgcc 
     5941 ttggtaggtc cagcggcgga ggaactcttt gatccggttc ctgaacagga tctatttgag 
     6001 gcgctaaatg aaaccttaac gctatggaac tcgccgcccg actgggctgg cgatgagcga 
     6061 aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg 
     6121 aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg cccagtatca gcccgtcata 
     6181 cttgaagcta gacaggctta tcttggacaa gaagaagatc gcttggcctc gcgcgcagat 
     6241 cagttggaag aatttgtcca ctacgtgaaa ggcgagatca ccaaggtagt cggcaaataa 
     6301 tgtctagcta gaaattcgtt caagccgacg ccgcttcgcg gcgcggctta actcaagtcg 
     6361 ttagatgcac taagcacata attgctcaca gccaaactat caggtcaagt ctgcttttat 
     6421 tatttttaag cgtgcataat aagccctaca caaattggga gatatatcat gcatgaccaa 
     6481 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg 
     6541 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc 
     6601 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac 
     6661 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca 
     6721 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt 
     6781 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc 
     6841 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg 
     6901 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc 
     6961 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac 
     7021 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct 
     7081 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc 
     7141 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt 
     7201 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac 
     7261 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg 
     7321 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac 
     7381 tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta 
     7441 cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg 
     7501 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg 
     7561 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agggtgcctt gatgtgggcg 
     7621 ccggcggtcg agtggcgacg gcgcggcttg tccgcgccct ggtagattgc ctggccctag 
     7681 gccagccatt tttgagcggc cagcggccgc gataggccga cgcgaagcgg cggggcgtag 
     7741 ggagcgcagc gaccgaaggg taggcgcttt ttgcagctct tcggctgtgc gctggccaga 
     7801 cagttatgca caggccaggc gggttttaag agttttaata agttttaaag agttttaggc 
     7861 ggaaaaatcg ccttttttct cttttatatc agtcacttac atgtgtgacc ggttcccaat 
     7921 gtacggcttt gggttcccaa tgtacgggtt ccggttccca atgtacggct ttgggttccc 
     7981 aatgtacgtg ctatccacag gaaagagacc ttttcgacct ttttcccctg ctagggcaat 
     8041 ttgccctagc atctgctccg tacattagga accggcggat gcttcgccct cgatcaggtt 
     8101 gcggtagcgc atgactagga tcgggccagc ctgccccgcc tcctccttca aatcgtactc 
     8161 cggcaggtca tttgacccga tcagcttgcg cacggtgaaa cagaacttct tgaactctcc 
     8221 ggcgctgcca ctgcgttcgt agatcgtctt gaacaaccat ctggcttctg ccttgcctgc 
     8281 ggcgcggcgt gccaggcggt agagaaaacg gccgatgccg ggatcgatca aaaagtaatc 
     8341 ggggtgaacc gtcagcacgt ccgggttctt gccttctgtg atctcgcggt acatccaatc 
     8401 agctagctcg atctcgatgt actccggccg cccggtttcg ctctttacga tcttgtagcg 
     8461 gctaatcaag gcttcaccct cggataccgt caccaggcgg ccgttcttgg ccttcttcgt 
     8521 acgctgcatg gcaacgtgcg tggtgtttaa ccgaatgcag gtttctacca ggtcgtcttt 
     8581 ctgctttccg ccatcggctc gccggcagaa cttgagtacg tccgcaacgt gtggacggaa 
     8641 cacgcggccg ggcttgtctc ccttcccttc ccggtatcgg ttcatggatt cggttagatg 
     8701 ggaaaccgcc atcagtacca ggtcgtaatc ccacacactg gccatgccgg ccggccctgc 
     8761 ggaaacctct acgtgcccgt ctggaagctc gtagcggatc acctcgccag ctcgtcggtc 
     8821 acgcttcgac agacggaaaa cggccacgtc catgatgctg cgactatcgc gggtgcccac 
     8881 gtcatagagc atcggaacga aaaaatctgg ttgctcgtcg cccttgggcg gcttcctaat 
     8941 cgacggcgca ccggctgccg gcggttgccg ggattctttg cggattcgat cagcggccgc 
     9001 ttgccacgat tcaccggggc gtgcttctgc ctcgatgcgt tgccgctggg cggcctgcgc 
     9061 ggccttcaac ttctccacca ggtcatcacc cagcgccgcg ccgatttgta ccgggccgga 
     9121 tggtttgcga ccgtcacgcc gattcctcgg gcttgggggt tccagtgcca ttgcagggcc 
     9181 ggcagacaac ccagccgctt acgcctggcc aaccgcccgt tcctccacac atggggcatt 
     9241 ccacggcgtc ggtgcctggt tgttcttgat tttccatgcc gcctccttta gccgctaaaa 
     9301 ttcatctact catttattca tttgctcatt tactctggta gctgcgcgat gtattcagat 
     9361 agcagctcgg taatggtctt gccttggcgt accgcgtaca tcttcagctt ggtgtgatcc 
     9421 tccgccggca actgaaagtt gacccgcttc atggctggcg tgtctgccag gctggccaac 
     9481 gttgcagcct tgctgctgcg tgcgctcgga cggccggcac ttagcgtgtt tgtgcttttg 
     9541 ctcattttct ctttacctca ttaactcaaa tgagttttga tttaatttca gcggccagcg 
     9601 cctggacctc gcgggcagcg tcgccctcgg gttctgattc aagaacggtt gtgccggcgg 
     9661 cggcagtgcc tgggtagctc acgcgctgcg tgatacggga ctcaagaatg ggcagctcgt 
     9721 acccggccag cgcctcggca acctcaccgc cgatgcgcgt gcctttgatc gcccgcgaca 
     9781 cgacaaaggc cgcttgtagc cttccatccg tgacctcaat gcgctgctta accagctcca 
     9841 ccaggtcggc ggtggcccat atgtcgtaag ggcttggctg caccggaatc agcacgaagt 
     9901 cggctgcctt gatcgcggac acagccaagt ccgccgcctg gggcgctccg tcgatcacta 
     9961 cgaagtcgcg ccggccgatg gccttcacgt cgcggtcaat cgtcgggcgg tcgatgccga 
    10021 caacggttag cggttgatct tcccgcacgg ccgcccaatc gcgggcactg ccctggggat 
    10081 cggaatcgac taacagaaca tcggccccgg cgagttgcag ggcgcgggct agatgggttg 
    10141 cgatggtcgt cttgcctgac ccgcctttct ggttaagtac agcgataacc ttcatgcgtt 
    10201 ccccttgcgt atttgtttat ttactcatcg catcatatac gcagcgaccg catgacgcaa 
    10261 gctgttttac tcaaatacac atcacctttt tagacggcgg cgctcggttt cttcagcggc 
    10321 caagctggcc ggccaggccg ccagcttggc atcagacaaa ccggccagga tttcatgcag 
    10381 ccgcacggtt gagacgtgcg cgggcggctc gaacacgtac ccggccgcga tcatctccgc 
    10441 ctcgatctct tcggtaatga aaaacggttc gtcctggccg tcctggtgcg gtttcatgct 
    10501 tgttcctctt ggcgttcatt ctcggcggcc gccagggcgt cggcctcggt caatgcgtcc 
    10561 tcacggaagg caccgcgccg cctggcctcg gtgggcgtca cttcctcgct gcgctcaagt 
    10621 gcgcggtaca gggtcgagcg atgcacgcca agcagtgcag ccgcctcttt cacggtgcgg 
    10681 ccttcctggt cgatcagctc gcgggcgtgc gcgatctgtg ccggggtgag ggtagggcgg 
    10741 gggccaaact tcacgcctcg ggccttggcg gcctcgcgcc cgctccgggt gcggtcgatg 
    10801 attagggaac gctcgaactc ggcaatgccg gcgaacacgg tcaacaccat gcggccggcc 
    10861 ggcgtggtgg tgtcggccca cggctctgcc aggctacgca ggcccgcgcc ggcctcctgg 
    10921 atgcgctcgg caatgtccag taggtcgcgg gtgctgcggg ccaggcggtc tagcctggtc 
    10981 actgtcacaa cgtcgccagg gcgtaggtgg tcaagcatcc tggccagctc cgggcggtcg 
    11041 cgcctggtgc cggtgatctt ctcggaaaac agcttggtgc agccggccgc gtgcagttcg 
    11101 gcccgttggt tggtcaagtc ctggtcgtcg gtgctgacgc gggcatagcc cagcaggcca 
    11161 gcggcggcgc tcttgttcat ggcgtaatgt ctccggttct agtcgcaagt attctacttt 
    11221 atgcgactaa aacacgcgac aagaaaacgc caggaaaagg gcagggcggc agcctgtcgc 
    11281 gtaacttagg acttgtgcga catgtcgttt tcagaagacg gctgcactga acgtcagaag 
    11341 ccgactgcac tatagcagcg gaggggttgg atcaaagtac tttaaagtac tttaaagtac 
    11401 tttaaagtac tttgatcccg aggggaaccc tgtggttggc atgcacatac aaatggacga 
    11461 acggataaac cttttcacgc ccttttaaat atccgttatt ctaataaacg ctcttttctc 
    11521 ttaggtttac ccgccaatat atcctgtcaa acactgatag tttaaactga aggcgggaaa 
    11581 cgacaatctg atccaagctc aagctgctct agccaatacg caaaccgcct ctccccgcgc 
    11641 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg 
    11701 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta 
    11761 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca 
    11821 gctatgacca tgattacgaa ttcgagctcg g 
//
