LOCUS       pUBN-GFP-DEST       11492 bp    DNA     circular     30-SEP-2010
SOURCE      
  ORGANISM  
REFERENCE   1  (bases 1 to 10818)
  AUTHORS   Grefen,C., Donald,N., Hashimoto,K., Kudla,J., Schumacher,K. and
            Blatt,M.R.
  TITLE     A ubiquitin-10 promoter-based vector set for fluorescent protein
            tagging facilitates temporal stability and native protein
            distribution in transient and stable expression studies
  JOURNAL   Plant J. 64 (2), 355-365 (2010)
   PUBMED   20735773
FEATURES             Location/Qualifiers
     misc_feature    complement(4535..4867)
                     /label=LB
     misc_feature    11052..11200
                     /label=RB
     terminator      3123..3331
                     /label=T35S
     CDS             3381..4531
                     /label=BarR
     promoter        1..639
                     /label=pUBQ10
     CDS             4931..5941
                     /label=SpecR
     misc_recomb     1395..1519
                     /label=attR1
     CDS             2628..2933
                     /label=ccdB
     misc_recomb     complement(2974..3098)
                     /label=attR2
     CDS             1628..2305
                     /label=CmR
     exon            666..1382
                     /label=GFP
     rep_origin      7482..10075
                     /label=pVS1
     rep_origin      6392..7072
                     /label=pBR322
BASE COUNT     2595 a      3091 c      3026 g      2780 t 
ORIGIN
        1 tacccgacga gtcagtaata aacggcgtca aagtggttgc agccggcaca cacgagtcgt 
       61 gtttatcaac tcaaagcaca aatacttttc ctcaacctaa aaataaggca attagccaaa 
      121 aacaactttg cgtgtaaaca acgctcaata cacgtgtcat tttattatta gctattgctt 
      181 caccgcctta gctttctcgt gacctagtcg tcctcgtctt ttcttcttct tcttctataa 
      241 aacaataccc aaagagctct tcttcttcac aattcagatt tcaatttctc aaaatcttaa 
      301 aaactttctc tcaattctct ctaccgtgat caaggtaaat ttctgtgttc cttattctct 
      361 caaaatcttc gattttgttt tcgttcgatc ccaatttcgt atatgttctt tggtttagat 
      421 tctgttaatc ttagatcgaa gacgattttc tgggtttgat cgttagatat catcttaatt 
      481 ctcgattagg gtttcataga tatcatccga tttgttcaaa taatttgagt tttgtcgaat 
      541 aattactctt cgatttgtga tttctatcta gatctggtgt tagtttctag tttgtgcgat 
      601 cgaatttgta gattaatctg agtttttctg attaacactc gagtgcggga tcctctagaa 
      661 ctagtatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc 
      721 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca 
      781 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc 
      841 ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca 
      901 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca 
      961 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca 
     1021 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg 
     1081 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga 
     1141 agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc 
     1201 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca 
     1261 accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca 
     1321 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca 
     1381 agcaattgtt aatcacaagt ttgtacaaaa aagctgaacg agaaacgtaa aatgatataa 
     1441 atatcaatat attaaattag attttgcata aaaaacagac tacataatac tgtaaaacac 
     1501 aacatatcca gtcactatgg cggccgcatt aggcacccca ggctttacac tttatgcttc 
     1561 cggctcgtat aatgtgtgga ttttgagtta ggatccgtcg agattttcag gagctaagga 
     1621 agctaaaatg gagaaaaaaa tcactggata taccaccgtt gatatatccc aatggcatcg 
     1681 taaagaacat tttgaggcat ttcagtcagt tgctcaatgt acctataacc agaccgttca 
     1741 gctggatatt acggcctttt taaagaccgt aaagaaaaat aagcacaagt tttatccggc 
     1801 ctttattcac attcttgccc gcctgatgaa tgctcatccg gaattccgta tggcaatgaa 
     1861 agacggtgag ctggtgatat gggatagtgt tcacccttgt tacaccgttt tccatgagca 
     1921 aactgaaacg ttttcatcgc tctggagtga ataccacgac gatttccggc agtttctaca 
     1981 catatattcg caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt 
     2041 tattgagaat atgtttttcg tctcagccaa tccctgggtg agtttcacca gttttgattt 
     2101 aaacgtggcc aatatggaca acttcttcgc ccccgttttc accatgggca aatattatac 
     2161 gcaaggcgac aaggtgctga tgccgctggc gattcaggtt catcatgccg tttgtgatgg 
     2221 cttccatgtc ggcagaatgc ttaatgaatt acaacagtac tgcgatgagt ggcaggcggg 
     2281 gcgtaatcta gaggatccgg cttactaaaa gccagataac agtatgcgta tttgcgcgct 
     2341 gatttttgcg gtataagaat atatactgat atgtataccc gaagtatgtc aaaaagaggt 
     2401 atgctatgaa gcagcgtatt acagtgacag ttgacagcga cagctatcag ttgctcaagg 
     2461 catatatgat gtcaatatct ccggtctggt aagcacaacc atgcagaatg aagcccgtcg 
     2521 tctgcgtgcc gaacgctgga aagcggaaaa tcaggaaggg atggctgagg tcgcccggtt 
     2581 tattgaaatg aacggctctt ttgctgacga gaacaggggc tggtgaaatg cagtttaagg 
     2641 tttacaccta taaaagagag agccgttatc gtctgtttgt ggatgtacag agtgatatta 
     2701 ttgacacgcc cgggcgacgg atggtgatcc ccctggccag tgcacgtctg ctgtcagata 
     2761 aagtctcccg tgaactttac ccggtggtgc atatcgggga tgaaagctgg cgcatgatga 
     2821 ccaccgatat ggccagtgtg ccggtctccg ttatcgggga agaagtggct gatctcagcc 
     2881 accgcgaaaa tgacatcaaa aacgccatta acctgatgtt ctggggaata taaatgtcag 
     2941 gctcccttat acacagccag tctgcaggtc gaccatagtg actggatatg ttgtgtttta 
     3001 cagtattatg tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca 
     3061 ttttacgttt ctcgttcagc tttcttgtac aaagtggtga ttaagcggcc atgctagagt 
     3121 ccgcaaaaat caccagtctc tctctacaaa tctatctctc tctatttttc tccagaataa 
     3181 tgtgtgagta gttcccagat aagggaatta gggttcttat agggtttcgc tcatgtgttg 
     3241 agcatataag aaacccttag tatgtatttg tatttgtaaa atacttctat caataaaatt 
     3301 tctaattcct aaaaccaaaa tccagtgacc tgcaggcatg cgacgtcggg ccctctagag 
     3361 gatccccggg taccgcgaat tatcgatcat gagcggagaa ttaagggagt cacgttatga 
     3421 cccccgccga tgacgcggga caagccgttt tacgtttgga actgacagaa ccgcaacgtt 
     3481 gaaggagcca ctgagccgcg ggtttctgga gtttaatgag ctaagcacat acgtcagaaa 
     3541 ccattattgc gcgttcaaaa gtcgcctaag gtcactatca gctagcaaat atttcttgtc 
     3601 aaaaatgctc cactgacgtt ccataaattc ccctcggtat ccaattagag tctcatattc 
     3661 actctcaact cgatcgaggg gatctaccat gagcccagaa cgacgcccgg ccgacatccg 
     3721 ccgtgccacc gaggcggaca tgccggcggt ctgcaccatc gtcaaccact acatcgagac 
     3781 aagcacggtc aacttccgta ccgagccgca ggaaccgcag gagtggacgg acgacctcgt 
     3841 ccgtctgcgg gagcgctatc cctggctcgt cgccgaggtg gacggcgagg tcgccggcat 
     3901 cgcctacgcg ggtccctgga aggcacgcaa cgcctacgac tggacggccg agtcgaccgt 
     3961 gtacgtctcc ccccgccacc agcggacggg actgggctcc acgctctaca cccacctgct 
     4021 gaagtccctg gaggcacagg gcttcaagag cgtggtcgct gtcatcgggc tgcccaacga 
     4081 cccgagcgtg cgcatgcacg aggcgctcgg atatgccccc cgcggcatgc tgcgggcggc 
     4141 cggcttcaag cacgggaact ggcatgacgt gggtttctgg cagctggact tcagcctgcc 
     4201 ggtgccgccc cgtccggtcc tgcccgtcac cgaaatctga tgacccctag agtcaagcag 
     4261 atcgttcaaa catttggcaa taaagtttct taagattgaa tcctgttgcc ggtcttgcga 
     4321 tgattatcat ataatttctg ttgaattacg ttaagcatgt aataattaac atgtaatgca 
     4381 tgacgttatt tatgagatgg gtttttatga ttagagtccc gcaattatac atttaatacg 
     4441 cgatagaaaa caaaatatag cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta 
     4501 tgttactaga tcgaccggca tgcaagctga taattcaatt cggcgttaat tcagtacatt 
     4561 aaaaacgtcc gcaatgtgtt attaagttgt ctaagcgtca atttgtttac accacaatat 
     4621 atcctgccac cagccagcca acagctcccc gaccggcagc tcggcacaaa atcaccactc 
     4681 gatacaggca gcccatcagt ccgggacggc gtcagcggga gagccgttgt aaggcggcag 
     4741 actttgctca tgttaccgat gctattcgga agaacggcaa ctaagctgcc gggtttgaaa 
     4801 cacggatgat ctcgcggagg gtagcatgtt gattgtaacg atgacagagc gttgctgcct 
     4861 gtgatcaatt cgggcacgaa cccagtggac ataagcctgt tcggttcgta agctgtaatg 
     4921 caagtagcgt atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa 
     4981 cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttggggtac agtctatgcc 
     5041 tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag 
     5101 caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatca tgggggaagc 
     5161 ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct 
     5221 cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc 
     5281 acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg 
     5341 agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg 
     5401 cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa 
     5461 gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc 
     5521 agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc 
     5581 cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga 
     5641 ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg 
     5701 aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc 
     5761 gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat 
     5821 acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga 
     5881 tcagttggaa gaatttgtcc actacgtgaa aggcgagatc accaaggtag tcggcaaata 
     5941 atgtctagct agaaattcgt tcaagccgac gccgcttcgc ggcgcggctt aactcaagtc 
     6001 gttagatgca ctaagcacat aattgctcac agccaaacta tcaggtcaag tctgctttta 
     6061 ttatttttaa gcgtgcataa taagccctac acaaattggg agatatatca tgcatgacca 
     6121 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag 
     6181 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac 
     6241 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa 
     6301 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc 
     6361 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag 
     6421 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac 
     6481 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc 
     6541 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc 
     6601 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca 
     6661 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc 
     6721 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg 
     6781 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct 
     6841 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata 
     6901 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc 
     6961 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca 
     7021 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct 
     7081 acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg 
     7141 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat 
     7201 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagggtgcct tgatgtgggc 
     7261 gccggcggtc gagtggcgac ggcgcggctt gtccgcgccc tggtagattg cctggcccta 
     7321 ggccagccat ttttgagcgg ccagcggccg cgataggccg acgcgaagcg gcggggcgta 
     7381 gggagcgcag cgaccgaagg gtaggcgctt tttgcagctc ttcggctgtg cgctggccag 
     7441 acagttatgc acaggccagg cgggttttaa gagttttaat aagttttaaa gagttttagg 
     7501 cggaaaaatc gccttttttc tcttttatat cagtcactta catgtgtgac cggttcccaa 
     7561 tgtacggctt tgggttccca atgtacgggt tccggttccc aatgtacggc tttgggttcc 
     7621 caatgtacgt gctatccaca ggaaagagac cttttcgacc tttttcccct gctagggcaa 
     7681 tttgccctag catctgctcc gtacattagg aaccggcgga tgcttcgccc tcgatcaggt 
     7741 tgcggtagcg catgactagg atcgggccag cctgccccgc ctcctccttc aaatcgtact 
     7801 ccggcaggtc atttgacccg atcagcttgc gcacggtgaa acagaacttc ttgaactctc 
     7861 cggcgctgcc actgcgttcg tagatcgtct tgaacaacca tctggcttct gccttgcctg 
     7921 cggcgcggcg tgccaggcgg tagagaaaac ggccgatgcc gggatcgatc aaaaagtaat 
     7981 cggggtgaac cgtcagcacg tccgggttct tgccttctgt gatctcgcgg tacatccaat 
     8041 cagctagctc gatctcgatg tactccggcc gcccggtttc gctctttacg atcttgtagc 
     8101 ggctaatcaa ggcttcaccc tcggataccg tcaccaggcg gccgttcttg gccttcttcg 
     8161 tacgctgcat ggcaacgtgc gtggtgttta accgaatgca ggtttctacc aggtcgtctt 
     8221 tctgctttcc gccatcggct cgccggcaga acttgagtac gtccgcaacg tgtggacgga 
     8281 acacgcggcc gggcttgtct cccttccctt cccggtatcg gttcatggat tcggttagat 
     8341 gggaaaccgc catcagtacc aggtcgtaat cccacacact ggccatgccg gccggccctg 
     8401 cggaaacctc tacgtgcccg tctggaagct cgtagcggat cacctcgcca gctcgtcggt 
     8461 cacgcttcga cagacggaaa acggccacgt ccatgatgct gcgactatcg cgggtgccca 
     8521 cgtcatagag catcggaacg aaaaaatctg gttgctcgtc gcccttgggc ggcttcctaa 
     8581 tcgacggcgc accggctgcc ggcggttgcc gggattcttt gcggattcga tcagcggccg 
     8641 cttgccacga ttcaccgggg cgtgcttctg cctcgatgcg ttgccgctgg gcggcctgcg 
     8701 cggccttcaa cttctccacc aggtcatcac ccagcgccgc gccgatttgt accgggccgg 
     8761 atggtttgcg accgtcacgc cgattcctcg ggcttggggg ttccagtgcc attgcagggc 
     8821 cggcagacaa cccagccgct tacgcctggc caaccgcccg ttcctccaca catggggcat 
     8881 tccacggcgt cggtgcctgg ttgttcttga ttttccatgc cgcctccttt agccgctaaa 
     8941 attcatctac tcatttattc atttgctcat ttactctggt agctgcgcga tgtattcaga 
     9001 tagcagctcg gtaatggtct tgccttggcg taccgcgtac atcttcagct tggtgtgatc 
     9061 ctccgccggc aactgaaagt tgacccgctt catggctggc gtgtctgcca ggctggccaa 
     9121 cgttgcagcc ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt ttgtgctttt 
     9181 gctcattttc tctttacctc attaactcaa atgagttttg atttaatttc agcggccagc 
     9241 gcctggacct cgcgggcagc gtcgccctcg ggttctgatt caagaacggt tgtgccggcg 
     9301 gcggcagtgc ctgggtagct cacgcgctgc gtgatacggg actcaagaat gggcagctcg 
     9361 tacccggcca gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat cgcccgcgac 
     9421 acgacaaagg ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt aaccagctcc 
     9481 accaggtcgg cggtggccca tatgtcgtaa gggcttggct gcaccggaat cagcacgaag 
     9541 tcggctgcct tgatcgcgga cacagccaag tccgccgcct ggggcgctcc gtcgatcact 
     9601 acgaagtcgc gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg gtcgatgccg 
     9661 acaacggtta gcggttgatc ttcccgcacg gccgcccaat cgcgggcact gccctgggga 
     9721 tcggaatcga ctaacagaac atcggccccg gcgagttgca gggcgcgggc tagatgggtt 
     9781 gcgatggtcg tcttgcctga cccgcctttc tggttaagta cagcgataac cttcatgcgt 
     9841 tccccttgcg tatttgttta tttactcatc gcatcatata cgcagcgacc gcatgacgca 
     9901 agctgtttta ctcaaataca catcaccttt ttagacggcg gcgctcggtt tcttcagcgg 
     9961 ccaagctggc cggccaggcc gccagcttgg catcagacaa accggccagg atttcatgca 
    10021 gccgcacggt tgagacgtgc gcgggcggct cgaacacgta cccggccgcg atcatctccg 
    10081 cctcgatctc ttcggtaatg aaaaacggtt cgtcctggcc gtcctggtgc ggtttcatgc 
    10141 ttgttcctct tggcgttcat tctcggcggc cgccagggcg tcggcctcgg tcaatgcgtc 
    10201 ctcacggaag gcaccgcgcc gcctggcctc ggtgggcgtc acttcctcgc tgcgctcaag 
    10261 tgcgcggtac agggtcgagc gatgcacgcc aagcagtgca gccgcctctt tcacggtgcg 
    10321 gccttcctgg tcgatcagct cgcgggcgtg cgcgatctgt gccggggtga gggtagggcg 
    10381 ggggccaaac ttcacgcctc gggccttggc ggcctcgcgc ccgctccggg tgcggtcgat 
    10441 gattagggaa cgctcgaact cggcaatgcc ggcgaacacg gtcaacacca tgcggccggc 
    10501 cggcgtggtg gtgtcggccc acggctctgc caggctacgc aggcccgcgc cggcctcctg 
    10561 gatgcgctcg gcaatgtcca gtaggtcgcg ggtgctgcgg gccaggcggt ctagcctggt 
    10621 cactgtcaca acgtcgccag ggcgtaggtg gtcaagcatc ctggccagct ccgggcggtc 
    10681 gcgcctggtg ccggtgatct tctcggaaaa cagcttggtg cagccggccg cgtgcagttc 
    10741 ggcccgttgg ttggtcaagt cctggtcgtc ggtgctgacg cgggcatagc ccagcaggcc 
    10801 agcggcggcg ctcttgttca tggcgtaatg tctccggttc tagtcgcaag tattctactt 
    10861 tatgcgacta aaacacgcga caagaaaacg ccaggaaaag ggcagggcgg cagcctgtcg 
    10921 cgtaacttag gacttgtgcg acatgtcgtt ttcagaagac ggctgcactg aacgtcagaa 
    10981 gccgactgca ctatagcagc ggaggggttg gatcaaagta ctttaaagta ctttaaagta 
    11041 ctttaaagta ctttgatccc gaggggaacc ctgtggttgg catgcacata caaatggacg 
    11101 aacggataaa ccttttcacg cccttttaaa tatccgttat tctaataaac gctcttttct 
    11161 cttaggttta cccgccaata tatcctgtca aacactgata gtttaaactg aaggcgggaa 
    11221 acgacaatct gatccaagct caagctgctc tagccaatac gcaaaccgcc tctccccgcg 
    11281 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt 
    11341 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt 
    11401 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac 
    11461 agctatgacc atgattacga attcgagctc gg 
//
