LOCUS       pUBN-EOS-DEST       11459 bp    DNA     circular     30-SEP-2010
SOURCE      
  ORGANISM  
REFERENCE   1  (bases 1 to 10818)
  AUTHORS   Grefen,C., Donald,N., Hashimoto,K., Kudla,J., Schumacher,K. and
            Blatt,M.R.
  TITLE     A ubiquitin-10 promoter-based vector set for fluorescent protein
            tagging facilitates temporal stability and native protein
            distribution in transient and stable expression studies
  JOURNAL   Plant J. 64 (2), 355-365 (2010)
   PUBMED   20735773
FEATURES             Location/Qualifiers
     misc_feature    complement(4502..4834)
                     /label=LB
     misc_feature    11019..11167
                     /label=RB
     terminator      3090..3298
                     /label=T35S
     CDS             3348..4498
                     /label=BarR
     promoter        1..639
                     /label=pUBQ10
     CDS             4898..5908
                     /label=SpecR
     misc_recomb     1362..1486
                     /label=attR1
     CDS             2595..2900
                     /label=ccdB
     misc_recomb     complement(2941..3065)
                     /label=attR2
     CDS             1595..2272
                     /label=CmR
     exon            675..1343
                     /label=EosFP
     rep_origin      6359..7039
                     /label=pBR322
     rep_origin      7449..10042
                     /label=pVS1
BASE COUNT     2621 a      2994 c      2996 g      2848 t 
ORIGIN
        1 tacccgacga gtcagtaata aacggcgtca aagtggttgc agccggcaca cacgagtcgt 
       61 gtttatcaac tcaaagcaca aatacttttc ctcaacctaa aaataaggca attagccaaa 
      121 aacaactttg cgtgtaaaca acgctcaata cacgtgtcat tttattatta gctattgctt 
      181 caccgcctta gctttctcgt gacctagtcg tcctcgtctt ttcttcttct tcttctataa 
      241 aacaataccc aaagagctct tcttcttcac aattcagatt tcaatttctc aaaatcttaa 
      301 aaactttctc tcaattctct ctaccgtgat caaggtaaat ttctgtgttc cttattctct 
      361 caaaatcttc gattttgttt tcgttcgatc ccaatttcgt atatgttctt tggtttagat 
      421 tctgttaatc ttagatcgaa gacgattttc tgggtttgat cgttagatat catcttaatt 
      481 ctcgattagg gtttcataga tatcatccga tttgttcaaa taatttgagt tttgtcgaat 
      541 aattactctt cgatttgtga tttctatcta gatctggtgt tagtttctag tttgtgcgat 
      601 cgaatttgta gattaatctg agtttttctg attaacactc gagtgcggga tcctctagaa 
      661 ctagtgtcgc caccatgagt gcgattaagc cagacatgaa gatcaacctc cgtatggaag 
      721 gcaacgtaaa cgggcaccac tttgtgatcg acggagatgg tacaggcaag ccttttgagg 
      781 gaaaacagag tatggatctt gaagtcaaag agggcggacc tctgcctttt gcctttgata 
      841 tcctgaccac tgcattccat tacggcaaca gggtattcgc cgaatatcca gaccacatac 
      901 aagactattt taagcagtcg tttcctaagg ggtattcgtg ggaacgaagc ttgactttcg 
      961 aagacggggg catttgcatt gccagaaacg acataacaat ggaaggggac actttctata 
     1021 ataaagttcg atttcacggt gtaaactttc ccgccaatgg tccagttatg cagaagaaga 
     1081 cgctgaaatg ggagccctcc actgagaaaa tgtatgtgcg tgatggagtg ctgactggtg 
     1141 atattcgcat ggctttgttg cttgaaggaa atgcccatta ccgatgtgac ttcagaacta 
     1201 cttacaaagc taaggagaag ggtgtcaagt taccaggcta ccactttgtg gaccactgca 
     1261 ttgagatttt aagccatgac aaagattaca acaaggttaa gctgtatgag catgctgttg 
     1321 ctcattctgg attgcctgac aatagacgac aattgttaat cacaagtttg tacaaaaaag 
     1381 ctgaacgaga aacgtaaaat gatataaata tcaatatatt aaattagatt ttgcataaaa 
     1441 aacagactac ataatactgt aaaacacaac atatccagtc actatggcgg ccgcattagg 
     1501 caccccaggc tttacacttt atgcttccgg ctcgtataat gtgtggattt tgagttagga 
     1561 tccgtcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac 
     1621 caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc 
     1681 tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa 
     1741 gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc 
     1801 tcatccggaa ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca 
     1861 cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata 
     1921 ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga 
     1981 aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc 
     2041 ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc 
     2101 cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat 
     2161 tcaggttcat catgccgttt gtgatggctt ccatgtcggc agaatgctta atgaattaca 
     2221 acagtactgc gatgagtggc aggcggggcg taatctagag gatccggctt actaaaagcc 
     2281 agataacagt atgcgtattt gcgcgctgat ttttgcggta taagaatata tactgatatg 
     2341 tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca gcgtattaca gtgacagttg 
     2401 acagcgacag ctatcagttg ctcaaggcat atatgatgtc aatatctccg gtctggtaag 
     2461 cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag cggaaaatca 
     2521 ggaagggatg gctgaggtcg cccggtttat tgaaatgaac ggctcttttg ctgacgagaa 
     2581 caggggctgg tgaaatgcag tttaaggttt acacctataa aagagagagc cgttatcgtc 
     2641 tgtttgtgga tgtacagagt gatattattg acacgcccgg gcgacggatg gtgatccccc 
     2701 tggccagtgc acgtctgctg tcagataaag tctcccgtga actttacccg gtggtgcata 
     2761 tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg gtctccgtta 
     2821 tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac gccattaacc 
     2881 tgatgttctg gggaatataa atgtcaggct cccttataca cagccagtct gcaggtcgac 
     2941 catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc 
     3001 taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt cttgtacaaa 
     3061 gtggtgatta agcggccatg ctagagtccg caaaaatcac cagtctctct ctacaaatct 
     3121 atctctctct atttttctcc agaataatgt gtgagtagtt cccagataag ggaattaggg 
     3181 ttcttatagg gtttcgctca tgtgttgagc atataagaaa cccttagtat gtatttgtat 
     3241 ttgtaaaata cttctatcaa taaaatttct aattcctaaa accaaaatcc agtgacctgc 
     3301 aggcatgcga cgtcgggccc tctagaggat ccccgggtac cgcgaattat cgatcatgag 
     3361 cggagaatta agggagtcac gttatgaccc ccgccgatga cgcgggacaa gccgttttac 
     3421 gtttggaact gacagaaccg caacgttgaa ggagccactg agccgcgggt ttctggagtt 
     3481 taatgagcta agcacatacg tcagaaacca ttattgcgcg ttcaaaagtc gcctaaggtc 
     3541 actatcagct agcaaatatt tcttgtcaaa aatgctccac tgacgttcca taaattcccc 
     3601 tcggtatcca attagagtct catattcact ctcaactcga tcgaggggat ctaccatgag 
     3661 cccagaacga cgcccggccg acatccgccg tgccaccgag gcggacatgc cggcggtctg 
     3721 caccatcgtc aaccactaca tcgagacaag cacggtcaac ttccgtaccg agccgcagga 
     3781 accgcaggag tggacggacg acctcgtccg tctgcgggag cgctatccct ggctcgtcgc 
     3841 cgaggtggac ggcgaggtcg ccggcatcgc ctacgcgggt ccctggaagg cacgcaacgc 
     3901 ctacgactgg acggccgagt cgaccgtgta cgtctccccc cgccaccagc ggacgggact 
     3961 gggctccacg ctctacaccc acctgctgaa gtccctggag gcacagggct tcaagagcgt 
     4021 ggtcgctgtc atcgggctgc ccaacgaccc gagcgtgcgc atgcacgagg cgctcggata 
     4081 tgccccccgc ggcatgctgc gggcggccgg cttcaagcac gggaactggc atgacgtggg 
     4141 tttctggcag ctggacttca gcctgccggt gccgccccgt ccggtcctgc ccgtcaccga 
     4201 aatctgatga cccctagagt caagcagatc gttcaaacat ttggcaataa agtttcttaa 
     4261 gattgaatcc tgttgccggt cttgcgatga ttatcatata atttctgttg aattacgtta 
     4321 agcatgtaat aattaacatg taatgcatga cgttatttat gagatgggtt tttatgatta 
     4381 gagtcccgca attatacatt taatacgcga tagaaaacaa aatatagcgc gcaaactagg 
     4441 ataaattatc gcgcgcggtg tcatctatgt tactagatcg accggcatgc aagctgataa 
     4501 ttcaattcgg cgttaattca gtacattaaa aacgtccgca atgtgttatt aagttgtcta 
     4561 agcgtcaatt tgtttacacc acaatatatc ctgccaccag ccagccaaca gctccccgac 
     4621 cggcagctcg gcacaaaatc accactcgat acaggcagcc catcagtccg ggacggcgtc 
     4681 agcgggagag ccgttgtaag gcggcagact ttgctcatgt taccgatgct attcggaaga 
     4741 acggcaacta agctgccggg tttgaaacac ggatgatctc gcggagggta gcatgttgat 
     4801 tgtaacgatg acagagcgtt gctgcctgtg atcaattcgg gcacgaaccc agtggacata 
     4861 agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg cgctcacgca actggtccag 
     4921 aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg cttgttatga 
     4981 ctgttttttt ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt 
     5041 gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca gtcgccctaa 
     5101 aacaaagtta aacatcatgg gggaagcggt gatcgccgaa gtatcgactc aactatcaga 
     5161 ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac atttgtacgg 
     5221 ctccgcagtg gatggcggcc tgaagccaca cagtgatatt gatttgctgg ttacggtgac 
     5281 cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg aaacttcggc 
     5341 ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg tgcacgacga 
     5401 catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa 
     5461 tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg ctatcttgct 
     5521 gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg aactctttga 
     5581 tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc tatggaactc 
     5641 gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc gcatttggta 
     5701 cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg caatggagcg 
     5761 cctgccggcc cagtatcagc ccgtcatact tgaagctaga caggcttatc ttggacaaga 
     5821 agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgtccact acgtgaaagg 
     5881 cgagatcacc aaggtagtcg gcaaataatg tctagctaga aattcgttca agccgacgcc 
     5941 gcttcgcggc gcggcttaac tcaagtcgtt agatgcacta agcacataat tgctcacagc 
     6001 caaactatca ggtcaagtct gcttttatta tttttaagcg tgcataataa gccctacaca 
     6061 aattgggaga tatatcatgc atgaccaaaa tcccttaacg tgagttttcg ttccactgag 
     6121 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa 
     6181 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag 
     6241 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg 
     6301 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat 
     6361 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta 
     6421 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg 
     6481 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc 
     6541 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa 
     6601 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc 
     6661 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt 
     6721 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct 
     6781 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc 
     6841 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg 
     6901 agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt 
     6961 gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt 
     7021 taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc 
     7081 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca 
     7141 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg 
     7201 cgcgaggcag ggtgccttga tgtgggcgcc ggcggtcgag tggcgacggc gcggcttgtc 
     7261 cgcgccctgg tagattgcct ggccctaggc cagccatttt tgagcggcca gcggccgcga 
     7321 taggccgacg cgaagcggcg gggcgtaggg agcgcagcga ccgaagggta ggcgcttttt 
     7381 gcagctcttc ggctgtgcgc tggccagaca gttatgcaca ggccaggcgg gttttaagag 
     7441 ttttaataag ttttaaagag ttttaggcgg aaaaatcgcc ttttttctct tttatatcag 
     7501 tcacttacat gtgtgaccgg ttcccaatgt acggctttgg gttcccaatg tacgggttcc 
     7561 ggttcccaat gtacggcttt gggttcccaa tgtacgtgct atccacagga aagagacctt 
     7621 ttcgaccttt ttcccctgct agggcaattt gccctagcat ctgctccgta cattaggaac 
     7681 cggcggatgc ttcgccctcg atcaggttgc ggtagcgcat gactaggatc gggccagcct 
     7741 gccccgcctc ctccttcaaa tcgtactccg gcaggtcatt tgacccgatc agcttgcgca 
     7801 cggtgaaaca gaacttcttg aactctccgg cgctgccact gcgttcgtag atcgtcttga 
     7861 acaaccatct ggcttctgcc ttgcctgcgg cgcggcgtgc caggcggtag agaaaacggc 
     7921 cgatgccggg atcgatcaaa aagtaatcgg ggtgaaccgt cagcacgtcc gggttcttgc 
     7981 cttctgtgat ctcgcggtac atccaatcag ctagctcgat ctcgatgtac tccggccgcc 
     8041 cggtttcgct ctttacgatc ttgtagcggc taatcaaggc ttcaccctcg gataccgtca 
     8101 ccaggcggcc gttcttggcc ttcttcgtac gctgcatggc aacgtgcgtg gtgtttaacc 
     8161 gaatgcaggt ttctaccagg tcgtctttct gctttccgcc atcggctcgc cggcagaact 
     8221 tgagtacgtc cgcaacgtgt ggacggaaca cgcggccggg cttgtctccc ttcccttccc 
     8281 ggtatcggtt catggattcg gttagatggg aaaccgccat cagtaccagg tcgtaatccc 
     8341 acacactggc catgccggcc ggccctgcgg aaacctctac gtgcccgtct ggaagctcgt 
     8401 agcggatcac ctcgccagct cgtcggtcac gcttcgacag acggaaaacg gccacgtcca 
     8461 tgatgctgcg actatcgcgg gtgcccacgt catagagcat cggaacgaaa aaatctggtt 
     8521 gctcgtcgcc cttgggcggc ttcctaatcg acggcgcacc ggctgccggc ggttgccggg 
     8581 attctttgcg gattcgatca gcggccgctt gccacgattc accggggcgt gcttctgcct 
     8641 cgatgcgttg ccgctgggcg gcctgcgcgg ccttcaactt ctccaccagg tcatcaccca 
     8701 gcgccgcgcc gatttgtacc gggccggatg gtttgcgacc gtcacgccga ttcctcgggc 
     8761 ttgggggttc cagtgccatt gcagggccgg cagacaaccc agccgcttac gcctggccaa 
     8821 ccgcccgttc ctccacacat ggggcattcc acggcgtcgg tgcctggttg ttcttgattt 
     8881 tccatgccgc ctcctttagc cgctaaaatt catctactca tttattcatt tgctcattta 
     8941 ctctggtagc tgcgcgatgt attcagatag cagctcggta atggtcttgc cttggcgtac 
     9001 cgcgtacatc ttcagcttgg tgtgatcctc cgccggcaac tgaaagttga cccgcttcat 
     9061 ggctggcgtg tctgccaggc tggccaacgt tgcagccttg ctgctgcgtg cgctcggacg 
     9121 gccggcactt agcgtgtttg tgcttttgct cattttctct ttacctcatt aactcaaatg 
     9181 agttttgatt taatttcagc ggccagcgcc tggacctcgc gggcagcgtc gccctcgggt 
     9241 tctgattcaa gaacggttgt gccggcggcg gcagtgcctg ggtagctcac gcgctgcgtg 
     9301 atacgggact caagaatggg cagctcgtac ccggccagcg cctcggcaac ctcaccgccg 
     9361 atgcgcgtgc ctttgatcgc ccgcgacacg acaaaggccg cttgtagcct tccatccgtg 
     9421 acctcaatgc gctgcttaac cagctccacc aggtcggcgg tggcccatat gtcgtaaggg 
     9481 cttggctgca ccggaatcag cacgaagtcg gctgccttga tcgcggacac agccaagtcc 
     9541 gccgcctggg gcgctccgtc gatcactacg aagtcgcgcc ggccgatggc cttcacgtcg 
     9601 cggtcaatcg tcgggcggtc gatgccgaca acggttagcg gttgatcttc ccgcacggcc 
     9661 gcccaatcgc gggcactgcc ctggggatcg gaatcgacta acagaacatc ggccccggcg 
     9721 agttgcaggg cgcgggctag atgggttgcg atggtcgtct tgcctgaccc gcctttctgg 
     9781 ttaagtacag cgataacctt catgcgttcc ccttgcgtat ttgtttattt actcatcgca 
     9841 tcatatacgc agcgaccgca tgacgcaagc tgttttactc aaatacacat caccttttta 
     9901 gacggcggcg ctcggtttct tcagcggcca agctggccgg ccaggccgcc agcttggcat 
     9961 cagacaaacc ggccaggatt tcatgcagcc gcacggttga gacgtgcgcg ggcggctcga 
    10021 acacgtaccc ggccgcgatc atctccgcct cgatctcttc ggtaatgaaa aacggttcgt 
    10081 cctggccgtc ctggtgcggt ttcatgcttg ttcctcttgg cgttcattct cggcggccgc 
    10141 cagggcgtcg gcctcggtca atgcgtcctc acggaaggca ccgcgccgcc tggcctcggt 
    10201 gggcgtcact tcctcgctgc gctcaagtgc gcggtacagg gtcgagcgat gcacgccaag 
    10261 cagtgcagcc gcctctttca cggtgcggcc ttcctggtcg atcagctcgc gggcgtgcgc 
    10321 gatctgtgcc ggggtgaggg tagggcgggg gccaaacttc acgcctcggg ccttggcggc 
    10381 ctcgcgcccg ctccgggtgc ggtcgatgat tagggaacgc tcgaactcgg caatgccggc 
    10441 gaacacggtc aacaccatgc ggccggccgg cgtggtggtg tcggcccacg gctctgccag 
    10501 gctacgcagg cccgcgccgg cctcctggat gcgctcggca atgtccagta ggtcgcgggt 
    10561 gctgcgggcc aggcggtcta gcctggtcac tgtcacaacg tcgccagggc gtaggtggtc 
    10621 aagcatcctg gccagctccg ggcggtcgcg cctggtgccg gtgatcttct cggaaaacag 
    10681 cttggtgcag ccggccgcgt gcagttcggc ccgttggttg gtcaagtcct ggtcgtcggt 
    10741 gctgacgcgg gcatagccca gcaggccagc ggcggcgctc ttgttcatgg cgtaatgtct 
    10801 ccggttctag tcgcaagtat tctactttat gcgactaaaa cacgcgacaa gaaaacgcca 
    10861 ggaaaagggc agggcggcag cctgtcgcgt aacttaggac ttgtgcgaca tgtcgttttc 
    10921 agaagacggc tgcactgaac gtcagaagcc gactgcacta tagcagcgga ggggttggat 
    10981 caaagtactt taaagtactt taaagtactt taaagtactt tgatcccgag gggaaccctg 
    11041 tggttggcat gcacatacaa atggacgaac ggataaacct tttcacgccc ttttaaatat 
    11101 ccgttattct aataaacgct cttttctctt aggtttaccc gccaatatat cctgtcaaac 
    11161 actgatagtt taaactgaag gcgggaaacg acaatctgat ccaagctcaa gctgctctag 
    11221 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac 
    11281 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact 
    11341 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg 
    11401 agcggataac aatttcacac aggaaacagc tatgaccatg attacgaatt cgagctcgg 
//
