LOCUS       pUBN-cYFP-DEST       11030 bp    DNA     circular     30-SEP-2010
SOURCE      
  ORGANISM  
REFERENCE   1  (bases 1 to 10818)
  AUTHORS   Grefen,C., Donald,N., Hashimoto,K., Kudla,J., Schumacher,K. and
            Blatt,M.R.
  TITLE     A ubiquitin-10 promoter-based vector set for fluorescent protein
            tagging facilitates temporal stability and native protein
            distribution in transient and stable expression studies
  JOURNAL   Plant J. 64 (2), 355-365 (2010)
   PUBMED   20735773
FEATURES             Location/Qualifiers
     misc_feature    complement(4073..4405)
                     /label=LB
     misc_feature    10590..10738
                     /label=RB
     terminator      2661..2869
                     /label=T35S
     CDS             2919..4069
                     /label=BarR
     promoter        1..639
                     /label=pUBQ10
     CDS             4469..5479
                     /label=SpecR
     misc_recomb     933..1057
                     /label=attR1
     CDS             2166..2471
                     /label=ccdB
     misc_recomb     complement(2512..2636)
                     /label=attR2
     CDS             1166..1843
                     /label=CmR
     exon            666..920
                     /label=cYFP
     rep_origin      5930..6610
                     /label=pBR322
     rep_origin      7020..9613
                     /label=pVS1
BASE COUNT     2489 a      2939 c      2891 g      2711 t 
ORIGIN
        1 tacccgacga gtcagtaata aacggcgtca aagtggttgc agccggcaca cacgagtcgt 
       61 gtttatcaac tcaaagcaca aatacttttc ctcaacctaa aaataaggca attagccaaa 
      121 aacaactttg cgtgtaaaca acgctcaata cacgtgtcat tttattatta gctattgctt 
      181 caccgcctta gctttctcgt gacctagtcg tcctcgtctt ttcttcttct tcttctataa 
      241 aacaataccc aaagagctct tcttcttcac aattcagatt tcaatttctc aaaatcttaa 
      301 aaactttctc tcaattctct ctaccgtgat caaggtaaat ttctgtgttc cttattctct 
      361 caaaatcttc gattttgttt tcgttcgatc ccaatttcgt atatgttctt tggtttagat 
      421 tctgttaatc ttagatcgaa gacgattttc tgggtttgat cgttagatat catcttaatt 
      481 ctcgattagg gtttcataga tatcatccga tttgttcaaa taatttgagt tttgtcgaat 
      541 aattactctt cgatttgtga tttctatcta gatctggtgt tagtttctag tttgtgcgat 
      601 cgaatttgta gattaatctg agtttttctg attaacactc gagtgcggga tcctctagaa 
      661 ctagtatgga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg 
      721 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc 
      781 ccgtgctgct gcccgacaac cactacctga gctaccagtc cgccctgagc aaagacccca 
      841 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg 
      901 gcatggacga gctgtacaag caattgttaa tcacaagttt gtacaaaaaa gctgaacgag 
      961 aaacgtaaaa tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta 
     1021 cataatactg taaaacacaa catatccagt cactatggcg gccgcattag gcaccccagg 
     1081 ctttacactt tatgcttccg gctcgtataa tgtgtggatt ttgagttagg atccgtcgag 
     1141 attttcagga gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga 
     1201 tatatcccaa tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac 
     1261 ctataaccag accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa 
     1321 gcacaagttt tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga 
     1381 attccgtatg gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta 
     1441 caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga 
     1501 tttccggcag tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc 
     1561 ctatttccct aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag 
     1621 tttcaccagt tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac 
     1681 catgggcaaa tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca 
     1741 tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg 
     1801 cgatgagtgg caggcggggc gtaatctaga ggatccggct tactaaaagc cagataacag 
     1861 tatgcgtatt tgcgcgctga tttttgcggt ataagaatat atactgatat gtatacccga 
     1921 agtatgtcaa aaagaggtat gctatgaagc agcgtattac agtgacagtt gacagcgaca 
     1981 gctatcagtt gctcaaggca tatatgatgt caatatctcc ggtctggtaa gcacaaccat 
     2041 gcagaatgaa gcccgtcgtc tgcgtgccga acgctggaaa gcggaaaatc aggaagggat 
     2101 ggctgaggtc gcccggttta ttgaaatgaa cggctctttt gctgacgaga acaggggctg 
     2161 gtgaaatgca gtttaaggtt tacacctata aaagagagag ccgttatcgt ctgtttgtgg 
     2221 atgtacagag tgatattatt gacacgcccg ggcgacggat ggtgatcccc ctggccagtg 
     2281 cacgtctgct gtcagataaa gtctcccgtg aactttaccc ggtggtgcat atcggggatg 
     2341 aaagctggcg catgatgacc accgatatgg ccagtgtgcc ggtctccgtt atcggggaag 
     2401 aagtggctga tctcagccac cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct 
     2461 ggggaatata aatgtcaggc tcccttatac acagccagtc tgcaggtcga ccatagtgac 
     2521 tggatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat ctaatttaat 
     2581 atattgatat ttatatcatt ttacgtttct cgttcagctt tcttgtacaa agtggtgatt 
     2641 aagcggccat gctagagtcc gcaaaaatca ccagtctctc tctacaaatc tatctctctc 
     2701 tatttttctc cagaataatg tgtgagtagt tcccagataa gggaattagg gttcttatag 
     2761 ggtttcgctc atgtgttgag catataagaa acccttagta tgtatttgta tttgtaaaat 
     2821 acttctatca ataaaatttc taattcctaa aaccaaaatc cagtgacctg caggcatgcg 
     2881 acgtcgggcc ctctagagga tccccgggta ccgcgaatta tcgatcatga gcggagaatt 
     2941 aagggagtca cgttatgacc cccgccgatg acgcgggaca agccgtttta cgtttggaac 
     3001 tgacagaacc gcaacgttga aggagccact gagccgcggg tttctggagt ttaatgagct 
     3061 aagcacatac gtcagaaacc attattgcgc gttcaaaagt cgcctaaggt cactatcagc 
     3121 tagcaaatat ttcttgtcaa aaatgctcca ctgacgttcc ataaattccc ctcggtatcc 
     3181 aattagagtc tcatattcac tctcaactcg atcgagggga tctaccatga gcccagaacg 
     3241 acgcccggcc gacatccgcc gtgccaccga ggcggacatg ccggcggtct gcaccatcgt 
     3301 caaccactac atcgagacaa gcacggtcaa cttccgtacc gagccgcagg aaccgcagga 
     3361 gtggacggac gacctcgtcc gtctgcggga gcgctatccc tggctcgtcg ccgaggtgga 
     3421 cggcgaggtc gccggcatcg cctacgcggg tccctggaag gcacgcaacg cctacgactg 
     3481 gacggccgag tcgaccgtgt acgtctcccc ccgccaccag cggacgggac tgggctccac 
     3541 gctctacacc cacctgctga agtccctgga ggcacagggc ttcaagagcg tggtcgctgt 
     3601 catcgggctg cccaacgacc cgagcgtgcg catgcacgag gcgctcggat atgccccccg 
     3661 cggcatgctg cgggcggccg gcttcaagca cgggaactgg catgacgtgg gtttctggca 
     3721 gctggacttc agcctgccgg tgccgccccg tccggtcctg cccgtcaccg aaatctgatg 
     3781 acccctagag tcaagcagat cgttcaaaca tttggcaata aagtttctta agattgaatc 
     3841 ctgttgccgg tcttgcgatg attatcatat aatttctgtt gaattacgtt aagcatgtaa 
     3901 taattaacat gtaatgcatg acgttattta tgagatgggt ttttatgatt agagtcccgc 
     3961 aattatacat ttaatacgcg atagaaaaca aaatatagcg cgcaaactag gataaattat 
     4021 cgcgcgcggt gtcatctatg ttactagatc gaccggcatg caagctgata attcaattcg 
     4081 gcgttaattc agtacattaa aaacgtccgc aatgtgttat taagttgtct aagcgtcaat 
     4141 ttgtttacac cacaatatat cctgccacca gccagccaac agctccccga ccggcagctc 
     4201 ggcacaaaat caccactcga tacaggcagc ccatcagtcc gggacggcgt cagcgggaga 
     4261 gccgttgtaa ggcggcagac tttgctcatg ttaccgatgc tattcggaag aacggcaact 
     4321 aagctgccgg gtttgaaaca cggatgatct cgcggagggt agcatgttga ttgtaacgat 
     4381 gacagagcgt tgctgcctgt gatcaattcg ggcacgaacc cagtggacat aagcctgttc 
     4441 ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac 
     4501 cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt 
     4561 tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg 
     4621 tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt 
     4681 aaacatcatg ggggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg 
     4741 cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt 
     4801 ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct 
     4861 tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg 
     4921 agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc 
     4981 gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct 
     5041 tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc 
     5101 aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc 
     5161 tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga 
     5221 ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt 
     5281 aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc 
     5341 ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg 
     5401 cttggcctcg cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac 
     5461 caaggtagtc ggcaaataat gtctagctag aaattcgttc aagccgacgc cgcttcgcgg 
     5521 cgcggcttaa ctcaagtcgt tagatgcact aagcacataa ttgctcacag ccaaactatc 
     5581 aggtcaagtc tgcttttatt atttttaagc gtgcataata agccctacac aaattgggag 
     5641 atatatcatg catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc 
     5701 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct 
     5761 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa 
     5821 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag 
     5881 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc 
     5941 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg 
     6001 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca 
     6061 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat 
     6121 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg 
     6181 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc 
     6241 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc 
     6301 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc 
     6361 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg 
     6421 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga 
     6481 gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt 
     6541 cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt 
     6601 atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc 
     6661 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac 
     6721 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca 
     6781 gggtgccttg atgtgggcgc cggcggtcga gtggcgacgg cgcggcttgt ccgcgccctg 
     6841 gtagattgcc tggccctagg ccagccattt ttgagcggcc agcggccgcg ataggccgac 
     6901 gcgaagcggc ggggcgtagg gagcgcagcg accgaagggt aggcgctttt tgcagctctt 
     6961 cggctgtgcg ctggccagac agttatgcac aggccaggcg ggttttaaga gttttaataa 
     7021 gttttaaaga gttttaggcg gaaaaatcgc cttttttctc ttttatatca gtcacttaca 
     7081 tgtgtgaccg gttcccaatg tacggctttg ggttcccaat gtacgggttc cggttcccaa 
     7141 tgtacggctt tgggttccca atgtacgtgc tatccacagg aaagagacct tttcgacctt 
     7201 tttcccctgc tagggcaatt tgccctagca tctgctccgt acattaggaa ccggcggatg 
     7261 cttcgccctc gatcaggttg cggtagcgca tgactaggat cgggccagcc tgccccgcct 
     7321 cctccttcaa atcgtactcc ggcaggtcat ttgacccgat cagcttgcgc acggtgaaac 
     7381 agaacttctt gaactctccg gcgctgccac tgcgttcgta gatcgtcttg aacaaccatc 
     7441 tggcttctgc cttgcctgcg gcgcggcgtg ccaggcggta gagaaaacgg ccgatgccgg 
     7501 gatcgatcaa aaagtaatcg gggtgaaccg tcagcacgtc cgggttcttg ccttctgtga 
     7561 tctcgcggta catccaatca gctagctcga tctcgatgta ctccggccgc ccggtttcgc 
     7621 tctttacgat cttgtagcgg ctaatcaagg cttcaccctc ggataccgtc accaggcggc 
     7681 cgttcttggc cttcttcgta cgctgcatgg caacgtgcgt ggtgtttaac cgaatgcagg 
     7741 tttctaccag gtcgtctttc tgctttccgc catcggctcg ccggcagaac ttgagtacgt 
     7801 ccgcaacgtg tggacggaac acgcggccgg gcttgtctcc cttcccttcc cggtatcggt 
     7861 tcatggattc ggttagatgg gaaaccgcca tcagtaccag gtcgtaatcc cacacactgg 
     7921 ccatgccggc cggccctgcg gaaacctcta cgtgcccgtc tggaagctcg tagcggatca 
     7981 cctcgccagc tcgtcggtca cgcttcgaca gacggaaaac ggccacgtcc atgatgctgc 
     8041 gactatcgcg ggtgcccacg tcatagagca tcggaacgaa aaaatctggt tgctcgtcgc 
     8101 ccttgggcgg cttcctaatc gacggcgcac cggctgccgg cggttgccgg gattctttgc 
     8161 ggattcgatc agcggccgct tgccacgatt caccggggcg tgcttctgcc tcgatgcgtt 
     8221 gccgctgggc ggcctgcgcg gccttcaact tctccaccag gtcatcaccc agcgccgcgc 
     8281 cgatttgtac cgggccggat ggtttgcgac cgtcacgccg attcctcggg cttgggggtt 
     8341 ccagtgccat tgcagggccg gcagacaacc cagccgctta cgcctggcca accgcccgtt 
     8401 cctccacaca tggggcattc cacggcgtcg gtgcctggtt gttcttgatt ttccatgccg 
     8461 cctcctttag ccgctaaaat tcatctactc atttattcat ttgctcattt actctggtag 
     8521 ctgcgcgatg tattcagata gcagctcggt aatggtcttg ccttggcgta ccgcgtacat 
     8581 cttcagcttg gtgtgatcct ccgccggcaa ctgaaagttg acccgcttca tggctggcgt 
     8641 gtctgccagg ctggccaacg ttgcagcctt gctgctgcgt gcgctcggac ggccggcact 
     8701 tagcgtgttt gtgcttttgc tcattttctc tttacctcat taactcaaat gagttttgat 
     8761 ttaatttcag cggccagcgc ctggacctcg cgggcagcgt cgccctcggg ttctgattca 
     8821 agaacggttg tgccggcggc ggcagtgcct gggtagctca cgcgctgcgt gatacgggac 
     8881 tcaagaatgg gcagctcgta cccggccagc gcctcggcaa cctcaccgcc gatgcgcgtg 
     8941 cctttgatcg cccgcgacac gacaaaggcc gcttgtagcc ttccatccgt gacctcaatg 
     9001 cgctgcttaa ccagctccac caggtcggcg gtggcccata tgtcgtaagg gcttggctgc 
     9061 accggaatca gcacgaagtc ggctgccttg atcgcggaca cagccaagtc cgccgcctgg 
     9121 ggcgctccgt cgatcactac gaagtcgcgc cggccgatgg ccttcacgtc gcggtcaatc 
     9181 gtcgggcggt cgatgccgac aacggttagc ggttgatctt cccgcacggc cgcccaatcg 
     9241 cgggcactgc cctggggatc ggaatcgact aacagaacat cggccccggc gagttgcagg 
     9301 gcgcgggcta gatgggttgc gatggtcgtc ttgcctgacc cgcctttctg gttaagtaca 
     9361 gcgataacct tcatgcgttc cccttgcgta tttgtttatt tactcatcgc atcatatacg 
     9421 cagcgaccgc atgacgcaag ctgttttact caaatacaca tcaccttttt agacggcggc 
     9481 gctcggtttc ttcagcggcc aagctggccg gccaggccgc cagcttggca tcagacaaac 
     9541 cggccaggat ttcatgcagc cgcacggttg agacgtgcgc gggcggctcg aacacgtacc 
     9601 cggccgcgat catctccgcc tcgatctctt cggtaatgaa aaacggttcg tcctggccgt 
     9661 cctggtgcgg tttcatgctt gttcctcttg gcgttcattc tcggcggccg ccagggcgtc 
     9721 ggcctcggtc aatgcgtcct cacggaaggc accgcgccgc ctggcctcgg tgggcgtcac 
     9781 ttcctcgctg cgctcaagtg cgcggtacag ggtcgagcga tgcacgccaa gcagtgcagc 
     9841 cgcctctttc acggtgcggc cttcctggtc gatcagctcg cgggcgtgcg cgatctgtgc 
     9901 cggggtgagg gtagggcggg ggccaaactt cacgcctcgg gccttggcgg cctcgcgccc 
     9961 gctccgggtg cggtcgatga ttagggaacg ctcgaactcg gcaatgccgg cgaacacggt 
    10021 caacaccatg cggccggccg gcgtggtggt gtcggcccac ggctctgcca ggctacgcag 
    10081 gcccgcgccg gcctcctgga tgcgctcggc aatgtccagt aggtcgcggg tgctgcgggc 
    10141 caggcggtct agcctggtca ctgtcacaac gtcgccaggg cgtaggtggt caagcatcct 
    10201 ggccagctcc gggcggtcgc gcctggtgcc ggtgatcttc tcggaaaaca gcttggtgca 
    10261 gccggccgcg tgcagttcgg cccgttggtt ggtcaagtcc tggtcgtcgg tgctgacgcg 
    10321 ggcatagccc agcaggccag cggcggcgct cttgttcatg gcgtaatgtc tccggttcta 
    10381 gtcgcaagta ttctacttta tgcgactaaa acacgcgaca agaaaacgcc aggaaaaggg 
    10441 cagggcggca gcctgtcgcg taacttagga cttgtgcgac atgtcgtttt cagaagacgg 
    10501 ctgcactgaa cgtcagaagc cgactgcact atagcagcgg aggggttgga tcaaagtact 
    10561 ttaaagtact ttaaagtact ttaaagtact ttgatcccga ggggaaccct gtggttggca 
    10621 tgcacataca aatggacgaa cggataaacc ttttcacgcc cttttaaata tccgttattc 
    10681 taataaacgc tcttttctct taggtttacc cgccaatata tcctgtcaaa cactgatagt 
    10741 ttaaactgaa ggcgggaaac gacaatctga tccaagctca agctgctcta gccaatacgc 
    10801 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc 
    10861 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca 
    10921 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa 
    10981 caatttcaca caggaaacag ctatgaccat gattacgaat tcgagctcgg 
//
