LOCUS       pUBC-YFP-DEST       11535 bp    DNA     circular     30-SEP-2010
SOURCE      
  ORGANISM  
REFERENCE   1  (bases 1 to 10818)
  AUTHORS   Grefen,C., Donald,N., Hashimoto,K., Kudla,J., Schumacher,K. and
            Blatt,M.R.
  TITLE     A ubiquitin-10 promoter-based vector set for fluorescent protein
            tagging facilitates temporal stability and native protein
            distribution in transient and stable expression studies
  JOURNAL   Plant J. 64 (2), 355-365 (2010)
   PUBMED   20735773
FEATURES             Location/Qualifiers
     CDS             901..1560
                     /label=CmR
     misc_recomb     668..792
                     /label=attR1
     misc_recomb     complement(2248..2372)
                     /label=attR2
     CDS             1902..2207
                     /label=ccdB
     misc_feature    complement(4578..4910)
                     /label=LB
     misc_feature    11095..11243
                     /label=RB
     terminator      3166..3374
                     /label=T35S
     CDS             3424..4574
                     /label=BarR
     promoter        1..639
                     /label=pUBQ10
     CDS             4974..5984
                     /label=SpecR
     exon            2425..3141
                     /label=YFP
     rep_origin      6435..7115
                     /label=pBR322
     rep_origin      7525..10118
                     /label=pVS1
BASE COUNT     2599 a      3106 c      3043 g      2787 t 
ORIGIN
        1 tacccgacga gtcagtaata aacggcgtca aagtggttgc agccggcaca cacgagtcgt 
       61 gtttatcaac tcaaagcaca aatacttttc ctcaacctaa aaataaggca attagccaaa 
      121 aacaactttg cgtgtaaaca acgctcaata cacgtgtcat tttattatta gctattgctt 
      181 caccgcctta gctttctcgt gacctagtcg tcctcgtctt ttcttcttct tcttctataa 
      241 aacaataccc aaagagctct tcttcttcac aattcagatt tcaatttctc aaaatcttaa 
      301 aaactttctc tcaattctct ctaccgtgat caaggtaaat ttctgtgttc cttattctct 
      361 caaaatcttc gattttgttt tcgttcgatc ccaatttcgt atatgttctt tggtttagat 
      421 tctgttaatc ttagatcgaa gacgattttc tgggtttgat cgttagatat catcttaatt 
      481 ctcgattagg gtttcataga tatcatccga tttgttcaaa taatttgagt tttgtcgaat 
      541 aattactctt cgatttgtga tttctatcta gatctggtgt tagtttctag tttgtgcgat 
      601 cgaatttgta gattaatctg agtttttctg attaacactc gagtgcggga tcctctaagg 
      661 gcccatcaca agtttgtaca aaaaagctga acgagaaacg taaaatgata taaatatcaa 
      721 tatattaaat tagattttgc ataaaaaaca gactacataa tactgtaaaa cacaacatat 
      781 ccagtcacta tggcggccgc attaggcacc ccaggcttta cactttatgc ttccggctcg 
      841 tataatgtgt ggattttgag ttaggatccg tcgagatttt caggagctaa ggaagctaaa 
      901 atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa 
      961 cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat 
     1021 attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc ggcctttatt 
     1081 cacattcttg cccgcctgat gaatgctcat ccggaattcc gtatggcaat gaaagacggt 
     1141 gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga gcaaactgaa 
     1201 acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct acacatatat 
     1261 tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg gtttattgag 
     1321 aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga tttaaacgtg 
     1381 gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc 
     1441 gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga tggcttccat 
     1501 gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggg cggggcgtaa 
     1561 acgcgtggat ccggcttact aaaagccaga taacagtatg cgtatttgcg cgctgatttt 
     1621 tgcggtataa gaatatatac tgatatgtat acccgaagta tgtcaaaaag aggtatgcta 
     1681 tgaagcagcg tattacagtg acagttgaca gcgacagcta tcagttgctc aaggcatata 
     1741 tgatgtcaat atctccggtc tggtaagcac aaccatgcag aatgaagccc gtcgtctgcg 
     1801 tgccgaacgc tggaaagcgg aaaatcagga agggatggct gaggtcgccc ggtttattga 
     1861 aatgaacggc tcttttgctg acgagaacag gggctggtga aatgcagttt aaggtttaca 
     1921 cctataaaag agagagccgt tatcgtctgt ttgtggatgt acagagtgat attattgaca 
     1981 cgcccgggcg acggatggtg atccccctgg ccagtgcacg tctgctgtca gataaagtct 
     2041 cccgtgaact ttacccggtg gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg 
     2101 atatggccag tgtgccggtc tccgttatcg gggaagaagt ggctgatctc agccaccgcg 
     2161 aaaatgacat caaaaacgcc attaacctga tgttctgggg aatataaatg tcaggctccc 
     2221 ttatacacag ccagtctgca ggtcgaccat agtgactgga tatgttgtgt tttacagtat 
     2281 tatgtagtct gttttttatg caaaatctaa tttaatatat tgatatttat atcattttac 
     2341 gtttctcgtt cagctttctt gtacaaagtg gtgatgggac gtccgcggag atctacgcgt 
     2401 gtcgactcga gatatccaac tagtatggtg agcaagggcg aggagctgtt caccggggtg 
     2461 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc 
     2521 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc 
     2581 aagctgcccg tgccctggcc caccctcgtg accaccttcg gctacggcct gcagtgcttc 
     2641 gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc 
     2701 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag 
     2761 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag 
     2821 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat 
     2881 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc 
     2941 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc 
     3001 cccgtgctgc tgcccgacaa ccactacctg agctaccagt ccgccctgag caaagacccc 
     3061 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc 
     3121 ggcatggacg agctgtacaa gttataagcg gccatgctag agtccgcaaa aatcaccagt 
     3181 ctctctctac aaatctatct ctctctattt ttctccagaa taatgtgtga gtagttccca 
     3241 gataagggaa ttagggttct tatagggttt cgctcatgtg ttgagcatat aagaaaccct 
     3301 tagtatgtat ttgtatttgt aaaatacttc tatcaataaa atttctaatt cctaaaacca 
     3361 aaatccagtg acctgcaggc atgcgacgtc gggccctcta gaggatcccc gggtaccgcg 
     3421 aattatcgat catgagcgga gaattaaggg agtcacgtta tgacccccgc cgatgacgcg 
     3481 ggacaagccg ttttacgttt ggaactgaca gaaccgcaac gttgaaggag ccactgagcc 
     3541 gcgggtttct ggagtttaat gagctaagca catacgtcag aaaccattat tgcgcgttca 
     3601 aaagtcgcct aaggtcacta tcagctagca aatatttctt gtcaaaaatg ctccactgac 
     3661 gttccataaa ttcccctcgg tatccaatta gagtctcata ttcactctca actcgatcga 
     3721 ggggatctac catgagccca gaacgacgcc cggccgacat ccgccgtgcc accgaggcgg 
     3781 acatgccggc ggtctgcacc atcgtcaacc actacatcga gacaagcacg gtcaacttcc 
     3841 gtaccgagcc gcaggaaccg caggagtgga cggacgacct cgtccgtctg cgggagcgct 
     3901 atccctggct cgtcgccgag gtggacggcg aggtcgccgg catcgcctac gcgggtccct 
     3961 ggaaggcacg caacgcctac gactggacgg ccgagtcgac cgtgtacgtc tccccccgcc 
     4021 accagcggac gggactgggc tccacgctct acacccacct gctgaagtcc ctggaggcac 
     4081 agggcttcaa gagcgtggtc gctgtcatcg ggctgcccaa cgacccgagc gtgcgcatgc 
     4141 acgaggcgct cggatatgcc ccccgcggca tgctgcgggc ggccggcttc aagcacggga 
     4201 actggcatga cgtgggtttc tggcagctgg acttcagcct gccggtgccg ccccgtccgg 
     4261 tcctgcccgt caccgaaatc tgatgacccc tagagtcaag cagatcgttc aaacatttgg 
     4321 caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt 
     4381 ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga 
     4441 tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata 
     4501 tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact agatcgaccg 
     4561 gcatgcaagc tgataattca attcggcgtt aattcagtac attaaaaacg tccgcaatgt 
     4621 gttattaagt tgtctaagcg tcaatttgtt tacaccacaa tatatcctgc caccagccag 
     4681 ccaacagctc cccgaccggc agctcggcac aaaatcacca ctcgatacag gcagcccatc 
     4741 agtccgggac ggcgtcagcg ggagagccgt tgtaaggcgg cagactttgc tcatgttacc 
     4801 gatgctattc ggaagaacgg caactaagct gccgggtttg aaacacggat gatctcgcgg 
     4861 agggtagcat gttgattgta acgatgacag agcgttgctg cctgtgatca attcgggcac 
     4921 gaacccagtg gacataagcc tgttcggttc gtaagctgta atgcaagtag cgtatgcgct 
     4981 cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt 
     5041 tcatggcttg ttatgactgt ttttttgggg tacagtctat gcctcgggca tccaagcagc 
     5101 aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc 
     5161 agggcagtcg ccctaaaaca aagttaaaca tcatggggga agcggtgatc gccgaagtat 
     5221 cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg 
     5281 ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagt gatattgatt 
     5341 tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc 
     5401 ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta gaagtcacca 
     5461 ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg 
     5521 gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg atcgacattg 
     5581 atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg 
     5641 cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct 
     5701 taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt 
     5761 tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg 
     5821 actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa gctagacagg 
     5881 cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg 
     5941 tccactacgt gaaaggcgag atcaccaagg tagtcggcaa ataatgtcta gctagaaatt 
     6001 cgttcaagcc gacgccgctt cgcggcgcgg cttaactcaa gtcgttagat gcactaagca 
     6061 cataattgct cacagccaaa ctatcaggtc aagtctgctt ttattatttt taagcgtgca 
     6121 taataagccc tacacaaatt gggagatata tcatgcatga ccaaaatccc ttaacgtgag 
     6181 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct 
     6241 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt 
     6301 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg 
     6361 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct 
     6421 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc 
     6481 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg 
     6541 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa 
     6601 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg 
     6661 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg 
     6721 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga 
     6781 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt 
     6841 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct 
     6901 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga 
     6961 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt 
     7021 ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc 
     7081 tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg 
     7141 ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg 
     7201 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac 
     7261 cgtcatcacc gaaacgcgcg aggcagggtg ccttgatgtg ggcgccggcg gtcgagtggc 
     7321 gacggcgcgg cttgtccgcg ccctggtaga ttgcctggcc ctaggccagc catttttgag 
     7381 cggccagcgg ccgcgatagg ccgacgcgaa gcggcggggc gtagggagcg cagcgaccga 
     7441 agggtaggcg ctttttgcag ctcttcggct gtgcgctggc cagacagtta tgcacaggcc 
     7501 aggcgggttt taagagtttt aataagtttt aaagagtttt aggcggaaaa atcgcctttt 
     7561 ttctctttta tatcagtcac ttacatgtgt gaccggttcc caatgtacgg ctttgggttc 
     7621 ccaatgtacg ggttccggtt cccaatgtac ggctttgggt tcccaatgta cgtgctatcc 
     7681 acaggaaaga gaccttttcg acctttttcc cctgctaggg caatttgccc tagcatctgc 
     7741 tccgtacatt aggaaccggc ggatgcttcg ccctcgatca ggttgcggta gcgcatgact 
     7801 aggatcgggc cagcctgccc cgcctcctcc ttcaaatcgt actccggcag gtcatttgac 
     7861 ccgatcagct tgcgcacggt gaaacagaac ttcttgaact ctccggcgct gccactgcgt 
     7921 tcgtagatcg tcttgaacaa ccatctggct tctgccttgc ctgcggcgcg gcgtgccagg 
     7981 cggtagagaa aacggccgat gccgggatcg atcaaaaagt aatcggggtg aaccgtcagc 
     8041 acgtccgggt tcttgccttc tgtgatctcg cggtacatcc aatcagctag ctcgatctcg 
     8101 atgtactccg gccgcccggt ttcgctcttt acgatcttgt agcggctaat caaggcttca 
     8161 ccctcggata ccgtcaccag gcggccgttc ttggccttct tcgtacgctg catggcaacg 
     8221 tgcgtggtgt ttaaccgaat gcaggtttct accaggtcgt ctttctgctt tccgccatcg 
     8281 gctcgccggc agaacttgag tacgtccgca acgtgtggac ggaacacgcg gccgggcttg 
     8341 tctcccttcc cttcccggta tcggttcatg gattcggtta gatgggaaac cgccatcagt 
     8401 accaggtcgt aatcccacac actggccatg ccggccggcc ctgcggaaac ctctacgtgc 
     8461 ccgtctggaa gctcgtagcg gatcacctcg ccagctcgtc ggtcacgctt cgacagacgg 
     8521 aaaacggcca cgtccatgat gctgcgacta tcgcgggtgc ccacgtcata gagcatcgga 
     8581 acgaaaaaat ctggttgctc gtcgcccttg ggcggcttcc taatcgacgg cgcaccggct 
     8641 gccggcggtt gccgggattc tttgcggatt cgatcagcgg ccgcttgcca cgattcaccg 
     8701 gggcgtgctt ctgcctcgat gcgttgccgc tgggcggcct gcgcggcctt caacttctcc 
     8761 accaggtcat cacccagcgc cgcgccgatt tgtaccgggc cggatggttt gcgaccgtca 
     8821 cgccgattcc tcgggcttgg gggttccagt gccattgcag ggccggcaga caacccagcc 
     8881 gcttacgcct ggccaaccgc ccgttcctcc acacatgggg cattccacgg cgtcggtgcc 
     8941 tggttgttct tgattttcca tgccgcctcc tttagccgct aaaattcatc tactcattta 
     9001 ttcatttgct catttactct ggtagctgcg cgatgtattc agatagcagc tcggtaatgg 
     9061 tcttgccttg gcgtaccgcg tacatcttca gcttggtgtg atcctccgcc ggcaactgaa 
     9121 agttgacccg cttcatggct ggcgtgtctg ccaggctggc caacgttgca gccttgctgc 
     9181 tgcgtgcgct cggacggccg gcacttagcg tgtttgtgct tttgctcatt ttctctttac 
     9241 ctcattaact caaatgagtt ttgatttaat ttcagcggcc agcgcctgga cctcgcgggc 
     9301 agcgtcgccc tcgggttctg attcaagaac ggttgtgccg gcggcggcag tgcctgggta 
     9361 gctcacgcgc tgcgtgatac gggactcaag aatgggcagc tcgtacccgg ccagcgcctc 
     9421 ggcaacctca ccgccgatgc gcgtgccttt gatcgcccgc gacacgacaa aggccgcttg 
     9481 tagccttcca tccgtgacct caatgcgctg cttaaccagc tccaccaggt cggcggtggc 
     9541 ccatatgtcg taagggcttg gctgcaccgg aatcagcacg aagtcggctg ccttgatcgc 
     9601 ggacacagcc aagtccgccg cctggggcgc tccgtcgatc actacgaagt cgcgccggcc 
     9661 gatggccttc acgtcgcggt caatcgtcgg gcggtcgatg ccgacaacgg ttagcggttg 
     9721 atcttcccgc acggccgccc aatcgcgggc actgccctgg ggatcggaat cgactaacag 
     9781 aacatcggcc ccggcgagtt gcagggcgcg ggctagatgg gttgcgatgg tcgtcttgcc 
     9841 tgacccgcct ttctggttaa gtacagcgat aaccttcatg cgttcccctt gcgtatttgt 
     9901 ttatttactc atcgcatcat atacgcagcg accgcatgac gcaagctgtt ttactcaaat 
     9961 acacatcacc tttttagacg gcggcgctcg gtttcttcag cggccaagct ggccggccag 
    10021 gccgccagct tggcatcaga caaaccggcc aggatttcat gcagccgcac ggttgagacg 
    10081 tgcgcgggcg gctcgaacac gtacccggcc gcgatcatct ccgcctcgat ctcttcggta 
    10141 atgaaaaacg gttcgtcctg gccgtcctgg tgcggtttca tgcttgttcc tcttggcgtt 
    10201 cattctcggc ggccgccagg gcgtcggcct cggtcaatgc gtcctcacgg aaggcaccgc 
    10261 gccgcctggc ctcggtgggc gtcacttcct cgctgcgctc aagtgcgcgg tacagggtcg 
    10321 agcgatgcac gccaagcagt gcagccgcct ctttcacggt gcggccttcc tggtcgatca 
    10381 gctcgcgggc gtgcgcgatc tgtgccgggg tgagggtagg gcgggggcca aacttcacgc 
    10441 ctcgggcctt ggcggcctcg cgcccgctcc gggtgcggtc gatgattagg gaacgctcga 
    10501 actcggcaat gccggcgaac acggtcaaca ccatgcggcc ggccggcgtg gtggtgtcgg 
    10561 cccacggctc tgccaggcta cgcaggcccg cgccggcctc ctggatgcgc tcggcaatgt 
    10621 ccagtaggtc gcgggtgctg cgggccaggc ggtctagcct ggtcactgtc acaacgtcgc 
    10681 cagggcgtag gtggtcaagc atcctggcca gctccgggcg gtcgcgcctg gtgccggtga 
    10741 tcttctcgga aaacagcttg gtgcagccgg ccgcgtgcag ttcggcccgt tggttggtca 
    10801 agtcctggtc gtcggtgctg acgcgggcat agcccagcag gccagcggcg gcgctcttgt 
    10861 tcatggcgta atgtctccgg ttctagtcgc aagtattcta ctttatgcga ctaaaacacg 
    10921 cgacaagaaa acgccaggaa aagggcaggg cggcagcctg tcgcgtaact taggacttgt 
    10981 gcgacatgtc gttttcagaa gacggctgca ctgaacgtca gaagccgact gcactatagc 
    11041 agcggagggg ttggatcaaa gtactttaaa gtactttaaa gtactttaaa gtactttgat 
    11101 cccgagggga accctgtggt tggcatgcac atacaaatgg acgaacggat aaaccttttc 
    11161 acgccctttt aaatatccgt tattctaata aacgctcttt tctcttaggt ttacccgcca 
    11221 atatatcctg tcaaacactg atagtttaaa ctgaaggcgg gaaacgacaa tctgatccaa 
    11281 gctcaagctg ctctagccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta 
    11341 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa 
    11401 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat 
    11461 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta 
    11521 cgaattcgag ctcgg 
//
