LOCUS       pUBC-RFP-DEST       11496 bp    DNA     circular     30-SEP-2010
SOURCE      
  ORGANISM  
REFERENCE   1  (bases 1 to 10818)
  AUTHORS   Grefen,C., Donald,N., Hashimoto,K., Kudla,J., Schumacher,K. and
            Blatt,M.R.
  TITLE     A ubiquitin-10 promoter-based vector set for fluorescent protein
            tagging facilitates temporal stability and native protein
            distribution in transient and stable expression studies
  JOURNAL   Plant J. 64 (2), 355-365 (2010)
   PUBMED   20735773
FEATURES             Location/Qualifiers
     CDS             901..1560
                     /label=CmR
     misc_recomb     668..792
                     /label=attR1
     misc_recomb     complement(2248..2372)
                     /label=attR2
     CDS             1902..2207
                     /label=ccdB
     misc_feature    complement(4539..4871)
                     /label=LB
     misc_feature    11056..11204
                     /label=RB
     terminator      3127..3335
                     /label=T35S
     CDS             3385..4535
                     /label=BarR
     promoter        1..639
                     /label=pUBQ10
     CDS             4935..5945
                     /label=SpecR
     exon            2425..3099
                     /label=RFP
     rep_origin      6396..7076
                     /label=pBR322
     rep_origin      7486..10079
                     /label=pVS1
BASE COUNT     2578 a      3094 c      3048 g      2776 t 
ORIGIN
        1 tacccgacga gtcagtaata aacggcgtca aagtggttgc agccggcaca cacgagtcgt 
       61 gtttatcaac tcaaagcaca aatacttttc ctcaacctaa aaataaggca attagccaaa 
      121 aacaactttg cgtgtaaaca acgctcaata cacgtgtcat tttattatta gctattgctt 
      181 caccgcctta gctttctcgt gacctagtcg tcctcgtctt ttcttcttct tcttctataa 
      241 aacaataccc aaagagctct tcttcttcac aattcagatt tcaatttctc aaaatcttaa 
      301 aaactttctc tcaattctct ctaccgtgat caaggtaaat ttctgtgttc cttattctct 
      361 caaaatcttc gattttgttt tcgttcgatc ccaatttcgt atatgttctt tggtttagat 
      421 tctgttaatc ttagatcgaa gacgattttc tgggtttgat cgttagatat catcttaatt 
      481 ctcgattagg gtttcataga tatcatccga tttgttcaaa taatttgagt tttgtcgaat 
      541 aattactctt cgatttgtga tttctatcta gatctggtgt tagtttctag tttgtgcgat 
      601 cgaatttgta gattaatctg agtttttctg attaacactc gagtgcggga tcctctaagg 
      661 gcccatcaca agtttgtaca aaaaagctga acgagaaacg taaaatgata taaatatcaa 
      721 tatattaaat tagattttgc ataaaaaaca gactacataa tactgtaaaa cacaacatat 
      781 ccagtcacta tggcggccgc attaggcacc ccaggcttta cactttatgc ttccggctcg 
      841 tataatgtgt ggattttgag ttaggatccg tcgagatttt caggagctaa ggaagctaaa 
      901 atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa 
      961 cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat 
     1021 attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc ggcctttatt 
     1081 cacattcttg cccgcctgat gaatgctcat ccggaattcc gtatggcaat gaaagacggt 
     1141 gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga gcaaactgaa 
     1201 acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct acacatatat 
     1261 tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg gtttattgag 
     1321 aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga tttaaacgtg 
     1381 gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc 
     1441 gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga tggcttccat 
     1501 gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggg cggggcgtaa 
     1561 acgcgtggat ccggcttact aaaagccaga taacagtatg cgtatttgcg cgctgatttt 
     1621 tgcggtataa gaatatatac tgatatgtat acccgaagta tgtcaaaaag aggtatgcta 
     1681 tgaagcagcg tattacagtg acagttgaca gcgacagcta tcagttgctc aaggcatata 
     1741 tgatgtcaat atctccggtc tggtaagcac aaccatgcag aatgaagccc gtcgtctgcg 
     1801 tgccgaacgc tggaaagcgg aaaatcagga agggatggct gaggtcgccc ggtttattga 
     1861 aatgaacggc tcttttgctg acgagaacag gggctggtga aatgcagttt aaggtttaca 
     1921 cctataaaag agagagccgt tatcgtctgt ttgtggatgt acagagtgat attattgaca 
     1981 cgcccgggcg acggatggtg atccccctgg ccagtgcacg tctgctgtca gataaagtct 
     2041 cccgtgaact ttacccggtg gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg 
     2101 atatggccag tgtgccggtc tccgttatcg gggaagaagt ggctgatctc agccaccgcg 
     2161 aaaatgacat caaaaacgcc attaacctga tgttctgggg aatataaatg tcaggctccc 
     2221 ttatacacag ccagtctgca ggtcgaccat agtgactgga tatgttgtgt tttacagtat 
     2281 tatgtagtct gttttttatg caaaatctaa tttaatatat tgatatttat atcattttac 
     2341 gtttctcgtt cagctttctt gtacaaagtg gtgatgggac gtccgcggag atctacgcgt 
     2401 gtcgactcga gatatccaac tagtatggcc tcctccgagg acgtcatcaa ggagttcatg 
     2461 cgcttcaagg tgcgcatgga gggctccgtg aacggccacg agttcgagat cgagggcgag 
     2521 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggcggc 
     2581 cccctgccct tcgcctggga catcctgtcc cctcagttcc agtacggctc caaggcctac 
     2641 gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag 
     2701 tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc 
     2761 ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac 
     2821 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct ccaccgagcg gatgtacccc 
     2881 gaggacggcg ccctgaaggg cgagatcaag atgaggctga agctgaagga cggcggccac 
     2941 tacgacgccg aggtcaagac cacctacatg gccaagaagc ccgtgcagct gcccggcgcc 
     3001 tacaagaccg acatcaagct ggacatcacc tcccacaacg aggactacac catcgtggaa 
     3061 cagtacgagc gcgccgaggg ccgccactcc accggcgcct aattataagc ggccatgcta 
     3121 gagtccgcaa aaatcaccag tctctctcta caaatctatc tctctctatt tttctccaga 
     3181 ataatgtgtg agtagttccc agataaggga attagggttc ttatagggtt tcgctcatgt 
     3241 gttgagcata taagaaaccc ttagtatgta tttgtatttg taaaatactt ctatcaataa 
     3301 aatttctaat tcctaaaacc aaaatccagt gacctgcagg catgcgacgt cgggccctct 
     3361 agaggatccc cgggtaccgc gaattatcga tcatgagcgg agaattaagg gagtcacgtt 
     3421 atgacccccg ccgatgacgc gggacaagcc gttttacgtt tggaactgac agaaccgcaa 
     3481 cgttgaagga gccactgagc cgcgggtttc tggagtttaa tgagctaagc acatacgtca 
     3541 gaaaccatta ttgcgcgttc aaaagtcgcc taaggtcact atcagctagc aaatatttct 
     3601 tgtcaaaaat gctccactga cgttccataa attcccctcg gtatccaatt agagtctcat 
     3661 attcactctc aactcgatcg aggggatcta ccatgagccc agaacgacgc ccggccgaca 
     3721 tccgccgtgc caccgaggcg gacatgccgg cggtctgcac catcgtcaac cactacatcg 
     3781 agacaagcac ggtcaacttc cgtaccgagc cgcaggaacc gcaggagtgg acggacgacc 
     3841 tcgtccgtct gcgggagcgc tatccctggc tcgtcgccga ggtggacggc gaggtcgccg 
     3901 gcatcgccta cgcgggtccc tggaaggcac gcaacgccta cgactggacg gccgagtcga 
     3961 ccgtgtacgt ctccccccgc caccagcgga cgggactggg ctccacgctc tacacccacc 
     4021 tgctgaagtc cctggaggca cagggcttca agagcgtggt cgctgtcatc gggctgccca 
     4081 acgacccgag cgtgcgcatg cacgaggcgc tcggatatgc cccccgcggc atgctgcggg 
     4141 cggccggctt caagcacggg aactggcatg acgtgggttt ctggcagctg gacttcagcc 
     4201 tgccggtgcc gccccgtccg gtcctgcccg tcaccgaaat ctgatgaccc ctagagtcaa 
     4261 gcagatcgtt caaacatttg gcaataaagt ttcttaagat tgaatcctgt tgccggtctt 
     4321 gcgatgatta tcatataatt tctgttgaat tacgttaagc atgtaataat taacatgtaa 
     4381 tgcatgacgt tatttatgag atgggttttt atgattagag tcccgcaatt atacatttaa 
     4441 tacgcgatag aaaacaaaat atagcgcgca aactaggata aattatcgcg cgcggtgtca 
     4501 tctatgttac tagatcgacc ggcatgcaag ctgataattc aattcggcgt taattcagta 
     4561 cattaaaaac gtccgcaatg tgttattaag ttgtctaagc gtcaatttgt ttacaccaca 
     4621 atatatcctg ccaccagcca gccaacagct ccccgaccgg cagctcggca caaaatcacc 
     4681 actcgataca ggcagcccat cagtccggga cggcgtcagc gggagagccg ttgtaaggcg 
     4741 gcagactttg ctcatgttac cgatgctatt cggaagaacg gcaactaagc tgccgggttt 
     4801 gaaacacgga tgatctcgcg gagggtagca tgttgattgt aacgatgaca gagcgttgct 
     4861 gcctgtgatc aattcgggca cgaacccagt ggacataagc ctgttcggtt cgtaagctgt 
     4921 aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg 
     4981 gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttggg gtacagtcta 
     5041 tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga 
     5101 gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac atcatggggg 
     5161 aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc 
     5221 atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga 
     5281 agccacacag tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc 
     5341 ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc 
     5401 tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag 
     5461 ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg 
     5521 agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg 
     5581 ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat 
     5641 ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg 
     5701 agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg 
     5761 cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg 
     5821 tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg 
     5881 cagatcagtt ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca 
     5941 aataatgtct agctagaaat tcgttcaagc cgacgccgct tcgcggcgcg gcttaactca 
     6001 agtcgttaga tgcactaagc acataattgc tcacagccaa actatcaggt caagtctgct 
     6061 tttattattt ttaagcgtgc ataataagcc ctacacaaat tgggagatat atcatgcatg 
     6121 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc 
     6181 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa 
     6241 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag 
     6301 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta 
     6361 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta 
     6421 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag 
     6481 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg 
     6541 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg 
     6601 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag 
     6661 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc 
     6721 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa 
     6781 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg 
     6841 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct 
     6901 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa 
     6961 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg 
     7021 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat 
     7081 cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct 
     7141 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct 
     7201 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagggt gccttgatgt 
     7261 gggcgccggc ggtcgagtgg cgacggcgcg gcttgtccgc gccctggtag attgcctggc 
     7321 cctaggccag ccatttttga gcggccagcg gccgcgatag gccgacgcga agcggcgggg 
     7381 cgtagggagc gcagcgaccg aagggtaggc gctttttgca gctcttcggc tgtgcgctgg 
     7441 ccagacagtt atgcacaggc caggcgggtt ttaagagttt taataagttt taaagagttt 
     7501 taggcggaaa aatcgccttt tttctctttt atatcagtca cttacatgtg tgaccggttc 
     7561 ccaatgtacg gctttgggtt cccaatgtac gggttccggt tcccaatgta cggctttggg 
     7621 ttcccaatgt acgtgctatc cacaggaaag agaccttttc gacctttttc ccctgctagg 
     7681 gcaatttgcc ctagcatctg ctccgtacat taggaaccgg cggatgcttc gccctcgatc 
     7741 aggttgcggt agcgcatgac taggatcggg ccagcctgcc ccgcctcctc cttcaaatcg 
     7801 tactccggca ggtcatttga cccgatcagc ttgcgcacgg tgaaacagaa cttcttgaac 
     7861 tctccggcgc tgccactgcg ttcgtagatc gtcttgaaca accatctggc ttctgccttg 
     7921 cctgcggcgc ggcgtgccag gcggtagaga aaacggccga tgccgggatc gatcaaaaag 
     7981 taatcggggt gaaccgtcag cacgtccggg ttcttgcctt ctgtgatctc gcggtacatc 
     8041 caatcagcta gctcgatctc gatgtactcc ggccgcccgg tttcgctctt tacgatcttg 
     8101 tagcggctaa tcaaggcttc accctcggat accgtcacca ggcggccgtt cttggccttc 
     8161 ttcgtacgct gcatggcaac gtgcgtggtg tttaaccgaa tgcaggtttc taccaggtcg 
     8221 tctttctgct ttccgccatc ggctcgccgg cagaacttga gtacgtccgc aacgtgtgga 
     8281 cggaacacgc ggccgggctt gtctcccttc ccttcccggt atcggttcat ggattcggtt 
     8341 agatgggaaa ccgccatcag taccaggtcg taatcccaca cactggccat gccggccggc 
     8401 cctgcggaaa cctctacgtg cccgtctgga agctcgtagc ggatcacctc gccagctcgt 
     8461 cggtcacgct tcgacagacg gaaaacggcc acgtccatga tgctgcgact atcgcgggtg 
     8521 cccacgtcat agagcatcgg aacgaaaaaa tctggttgct cgtcgccctt gggcggcttc 
     8581 ctaatcgacg gcgcaccggc tgccggcggt tgccgggatt ctttgcggat tcgatcagcg 
     8641 gccgcttgcc acgattcacc ggggcgtgct tctgcctcga tgcgttgccg ctgggcggcc 
     8701 tgcgcggcct tcaacttctc caccaggtca tcacccagcg ccgcgccgat ttgtaccggg 
     8761 ccggatggtt tgcgaccgtc acgccgattc ctcgggcttg ggggttccag tgccattgca 
     8821 gggccggcag acaacccagc cgcttacgcc tggccaaccg cccgttcctc cacacatggg 
     8881 gcattccacg gcgtcggtgc ctggttgttc ttgattttcc atgccgcctc ctttagccgc 
     8941 taaaattcat ctactcattt attcatttgc tcatttactc tggtagctgc gcgatgtatt 
     9001 cagatagcag ctcggtaatg gtcttgcctt ggcgtaccgc gtacatcttc agcttggtgt 
     9061 gatcctccgc cggcaactga aagttgaccc gcttcatggc tggcgtgtct gccaggctgg 
     9121 ccaacgttgc agccttgctg ctgcgtgcgc tcggacggcc ggcacttagc gtgtttgtgc 
     9181 ttttgctcat tttctcttta cctcattaac tcaaatgagt tttgatttaa tttcagcggc 
     9241 cagcgcctgg acctcgcggg cagcgtcgcc ctcgggttct gattcaagaa cggttgtgcc 
     9301 ggcggcggca gtgcctgggt agctcacgcg ctgcgtgata cgggactcaa gaatgggcag 
     9361 ctcgtacccg gccagcgcct cggcaacctc accgccgatg cgcgtgcctt tgatcgcccg 
     9421 cgacacgaca aaggccgctt gtagccttcc atccgtgacc tcaatgcgct gcttaaccag 
     9481 ctccaccagg tcggcggtgg cccatatgtc gtaagggctt ggctgcaccg gaatcagcac 
     9541 gaagtcggct gccttgatcg cggacacagc caagtccgcc gcctggggcg ctccgtcgat 
     9601 cactacgaag tcgcgccggc cgatggcctt cacgtcgcgg tcaatcgtcg ggcggtcgat 
     9661 gccgacaacg gttagcggtt gatcttcccg cacggccgcc caatcgcggg cactgccctg 
     9721 gggatcggaa tcgactaaca gaacatcggc cccggcgagt tgcagggcgc gggctagatg 
     9781 ggttgcgatg gtcgtcttgc ctgacccgcc tttctggtta agtacagcga taaccttcat 
     9841 gcgttcccct tgcgtatttg tttatttact catcgcatca tatacgcagc gaccgcatga 
     9901 cgcaagctgt tttactcaaa tacacatcac ctttttagac ggcggcgctc ggtttcttca 
     9961 gcggccaagc tggccggcca ggccgccagc ttggcatcag acaaaccggc caggatttca 
    10021 tgcagccgca cggttgagac gtgcgcgggc ggctcgaaca cgtacccggc cgcgatcatc 
    10081 tccgcctcga tctcttcggt aatgaaaaac ggttcgtcct ggccgtcctg gtgcggtttc 
    10141 atgcttgttc ctcttggcgt tcattctcgg cggccgccag ggcgtcggcc tcggtcaatg 
    10201 cgtcctcacg gaaggcaccg cgccgcctgg cctcggtggg cgtcacttcc tcgctgcgct 
    10261 caagtgcgcg gtacagggtc gagcgatgca cgccaagcag tgcagccgcc tctttcacgg 
    10321 tgcggccttc ctggtcgatc agctcgcggg cgtgcgcgat ctgtgccggg gtgagggtag 
    10381 ggcgggggcc aaacttcacg cctcgggcct tggcggcctc gcgcccgctc cgggtgcggt 
    10441 cgatgattag ggaacgctcg aactcggcaa tgccggcgaa cacggtcaac accatgcggc 
    10501 cggccggcgt ggtggtgtcg gcccacggct ctgccaggct acgcaggccc gcgccggcct 
    10561 cctggatgcg ctcggcaatg tccagtaggt cgcgggtgct gcgggccagg cggtctagcc 
    10621 tggtcactgt cacaacgtcg ccagggcgta ggtggtcaag catcctggcc agctccgggc 
    10681 ggtcgcgcct ggtgccggtg atcttctcgg aaaacagctt ggtgcagccg gccgcgtgca 
    10741 gttcggcccg ttggttggtc aagtcctggt cgtcggtgct gacgcgggca tagcccagca 
    10801 ggccagcggc ggcgctcttg ttcatggcgt aatgtctccg gttctagtcg caagtattct 
    10861 actttatgcg actaaaacac gcgacaagaa aacgccagga aaagggcagg gcggcagcct 
    10921 gtcgcgtaac ttaggacttg tgcgacatgt cgttttcaga agacggctgc actgaacgtc 
    10981 agaagccgac tgcactatag cagcggaggg gttggatcaa agtactttaa agtactttaa 
    11041 agtactttaa agtactttga tcccgagggg aaccctgtgg ttggcatgca catacaaatg 
    11101 gacgaacgga taaacctttt cacgcccttt taaatatccg ttattctaat aaacgctctt 
    11161 ttctcttagg tttacccgcc aatatatcct gtcaaacact gatagtttaa actgaaggcg 
    11221 ggaaacgaca atctgatcca agctcaagct gctctagcca atacgcaaac cgcctctccc 
    11281 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg 
    11341 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca 
    11401 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg 
    11461 aaacagctat gaccatgatt acgaattcga gctcgg 
//
