LOCUS       pUBC-nYFP-Dest       11924 bp    DNA     circular     28-AUG-2015
SOURCE      
  ORGANISM  
REFERENCE   1  (bases 1 to 10818)
  AUTHORS   Grefen,C., Donald,N., Hashimoto,K., Kudla,J., Schumacher,K. and
            Blatt,M.R.
  TITLE     A ubiquitin-10 promoter-based vector set for fluorescent protein
            tagging facilitates temporal stability and native protein
            distribution in transient and stable expression studies
  JOURNAL   Plant J. 64 (2), 355-365 (2010)
   PUBMED   20735773
FEATURES             Location/Qualifiers
     misc_feature    complement(4967..5299)
                     /label=LB
     misc_feature    11484..11632
                     /label=RB
     promoter        1..639
                     /label=pUBQ10
     CDS             5363..6373
                     /label=SpecR
     CDS             3174..4963
                     /label=HygR
     CDS             901..1560
                     /label=CmR
     misc_recomb     668..792
                     /label=attR1
     misc_recomb     complement(2248..2372)
                     /label=attR2
     CDS             1902..2207
                     /label=ccdB
     terminator      2914..3122
                     /label=T35S
     exon            2425..2889
                     /label=nYFP
     rep_origin      6824..7504
                     /label=ori (pMB1)
     rep_origin      7914..10507
                     /label=pVS1
ORIGIN
        1 tacccgacga gtcagtaata aacggcgtca aagtggttgc agccggcaca cacgagtcgt 
       61 gtttatcaac tcaaagcaca aatacttttc ctcaacctaa aaataaggca attagccaaa 
      121 aacaactttg cgtgtaaaca acgctcaata cacgtgtcat tttattatta gctattgctt 
      181 caccgcctta gctttctcgt gacctagtcg tcctcgtctt ttcttcttct tcttctataa 
      241 aacaataccc aaagagctct tcttcttcac aattcagatt tcaatttctc aaaatcttaa 
      301 aaactttctc tcaattctct ctaccgtgat caaggtaaat ttctgtgttc cttattctct 
      361 caaaatcttc gattttgttt tcgttcgatc ccaatttcgt atatgttctt tggtttagat 
      421 tctgttaatc ttagatcgaa gacgattttc tgggtttgat cgttagatat catcttaatt 
      481 ctcgattagg gtttcataga tatcatccga tttgttcaaa taatttgagt tttgtcgaat 
      541 aattactctt cgatttgtga tttctatcta gatctggtgt tagtttctag tttgtgcgat 
      601 cgaatttgta gattaatctg agtttttctg attaacactc gagtgcggga tcctctaagg 
      661 gcccatcaca agtttgtaca aaaaagctga acgagaaacg taaaatgata taaatatcaa 
      721 tatattaaat tagattttgc ataaaaaaca gactacataa tactgtaaaa cacaacatat 
      781 ccagtcacta tggcggccgc attaggcacc ccaggcttta cactttatgc ttccggctcg 
      841 tataatgtgt ggattttgag ttaggatccg tcgagatttt caggagctaa ggaagctaaa 
      901 atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa 
      961 cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat 
     1021 attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc ggcctttatt 
     1081 cacattcttg cccgcctgat gaatgctcat ccggaattcc gtatggcaat gaaagacggt 
     1141 gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga gcaaactgaa 
     1201 acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct acacatatat 
     1261 tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg gtttattgag 
     1321 aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga tttaaacgtg 
     1381 gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc 
     1441 gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga tggcttccat 
     1501 gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggg cggggcgtaa 
     1561 acgcgtggat ccggcttact aaaagccaga taacagtatg cgtatttgcg cgctgatttt 
     1621 tgcggtataa gaatatatac tgatatgtat acccgaagta tgtcaaaaag aggtatgcta 
     1681 tgaagcagcg tattacagtg acagttgaca gcgacagcta tcagttgctc aaggcatata 
     1741 tgatgtcaat atctccggtc tggtaagcac aaccatgcag aatgaagccc gtcgtctgcg 
     1801 tgccgaacgc tggaaagcgg aaaatcagga agggatggct gaggtcgccc ggtttattga 
     1861 aatgaacggc tcttttgctg acgagaacag gggctggtga aatgcagttt aaggtttaca 
     1921 cctataaaag agagagccgt tatcgtctgt ttgtggatgt acagagtgat attattgaca 
     1981 cgcccgggcg acggatggtg atccccctgg ccagtgcacg tctgctgtca gataaagtct 
     2041 cccgtgaact ttacccggtg gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg 
     2101 atatggccag tgtgccggtc tccgttatcg gggaagaagt ggctgatctc agccaccgcg 
     2161 aaaatgacat caaaaacgcc attaacctga tgttctgggg aatataaatg tcaggctccc 
     2221 ttatacacag ccagtctgca ggtcgaccat agtgactgga tatgttgtgt tttacagtat 
     2281 tatgtagtct gttttttatg caaaatctaa tttaatatat tgatatttat atcattttac 
     2341 gtttctcgtt cagctttctt gtacaaagtg gtgatgggac gtccgcggag atctacgcgt 
     2401 gtcgactcga gatatccaac tagtatggtg agcaagggcg aggagctgtt caccggggtg 
     2461 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc 
     2521 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc 
     2581 aagctgcccg tgccctggcc caccctcgtg accaccttcg gctacggcct gcagtgcttc 
     2641 gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc 
     2701 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag 
     2761 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag 
     2821 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat 
     2881 atcatggcct tataagcggc catgctagag tccgcaaaaa tcaccagtct ctctctacaa 
     2941 atctatctct ctctattttt ctccagaata atgtgtgagt agttcccaga taagggaatt 
     3001 agggttctta tagggtttcg ctcatgtgtt gagcatataa gaaaccctta gtatgtattt 
     3061 gtatttgtaa aatacttcta tcaataaaat ttctaattcc taaaaccaaa atccagtgac 
     3121 ctgcaggcat gcgacgtcgg gccctctaga ggatccccgg gtaccctcga attatcatac 
     3181 atgagaatta agggagtcac gttatgaccc cgccgatgac gcgggacaag ccgttttacg 
     3241 tttggaactg acagaaccgc aacgttgaag gagccactga gccgcgggtt tctggagttt 
     3301 aatgagctaa gcacatacgt cagaaaccat tattgcgcgt tcaaaagtcg cctaaggtca 
     3361 ctatcagcta gcaaatattt cttgtcaaaa atgctccact gacgttccat aaattcccct 
     3421 cggtatccaa ttagagtctc atattcactc tcaactcgaa tcccccctat gaaaaagcct 
     3481 gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag cgtctccgac 
     3541 ctgatgcagc tctcggaggg cgaagaatct cgtgctttca gcttcgatgt aggagggcgt 
     3601 ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg ttatgtttat 
     3661 cggcactttg catcggccgc gctcccgatt ccggaagtgc ttgacattgg ggagttcagc 
     3721 gagagcctga cctattgcat ctcccgccgt gcacagggtg tcacgttgca agacctgcct 
     3781 gaaaccgaac tgcccgctgt tcttcagccg gtcgcggagg ctatggatgc tatcgctgcg 
     3841 gccgatctta gccagacgag cgggttcggc ccattcggac cgcaaggaat cggtcaatac 
     3901 actacatggc gtgatttcat atgcgcgatt gctgatcccc atgtgtatca ctggcaaact 
     3961 gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg 
     4021 gccgaggact gccccgaagt ccggcacctc gtgcacgcgg atttcggctc caacaatgtc 
     4081 ctgacggaca atggccgcat aacagcggtc attgactgga gcgaggcgat gttcggggat 
     4141 tcccaatacg aggtcgccaa catcttcttc tggaggccgt ggttggcttg tatggagcag 
     4201 cagacgcgct acttcgagcg gaggcatccg gagcttgcag gatcgccaag gctccgggcg 
     4261 tatatgctcc gcattggtct tgaccaactc tatcagagct tggttgacgg caatttcgat 
     4321 gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc cgggactgtc 
     4381 gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg tgtagaagta 
     4441 ctcgccgata gtggaaaccg acgccccagc actcgtccga gggcaaagga atagagtaga 
     4501 tgccgaccga acaagagctg atttcgagaa cgcctcagcc agcaactcgc gcgagcctag 
     4561 caaggcaaat gcgagagaac ggccttacgc ttggtggcac agttctcgtc cacagttcgc 
     4621 taagctcgct cggctgggtc gcgggagggc cggtcgcagt gattcaggaa ttaattccct 
     4681 agagtcaagc agatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg 
     4741 ccggtcttgc gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta 
     4801 acatgtaatg catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat 
     4861 acatttaata cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg 
     4921 cggtgtcatc tatgttacta gatcgaccgg catgcaagct gataattcaa ttcggcgtta 
     4981 attcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt caatttgttt 
     5041 acaccacaat atatcctgcc accagccagc caacagctcc ccgaccggca gctcggcaca 
     5101 aaatcaccac tcgatacagg cagcccatca gtccgggacg gcgtcagcgg gagagccgtt 
     5161 gtaaggcggc agactttgct catgttaccg atgctattcg gaagaacggc aactaagctg 
     5221 ccgggtttga aacacggatg atctcgcgga gggtagcatg ttgattgtaa cgatgacaga 
     5281 gcgttgctgc ctgtgatcaa ttcgggcacg aacccagtgg acataagcct gttcggttcg 
     5341 taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg tccagaacct tgaccgaacg 
     5401 cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttggggt 
     5461 acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat 
     5521 gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttaaacat 
     5581 catgggggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat 
     5641 cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg 
     5701 cggcctgaag ccacacagtg atattgattt gctggttacg gtgaccgtaa ggcttgatga 
     5761 aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc ctggagagag 
     5821 cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca ttccgtggcg 
     5881 ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg 
     5941 tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa aagcaagaga 
     6001 acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg ttcctgaaca 
     6061 ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc ccgactgggc 
     6121 tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg 
     6181 caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc cggcccagta 
     6241 tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag atcgcttggc 
     6301 ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt 
     6361 agtcggcaaa taatgtctag ctagaaattc gttcaagccg acgccgcttc gcggcgcggc 
     6421 ttaactcaag tcgttagatg cactaagcac ataattgctc acagccaaac tatcaggtca 
     6481 agtctgcttt tattattttt aagcgtgcat aataagccct acacaaattg ggagatatat 
     6541 catgcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag 
     6601 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa 
     6661 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt 
     6721 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc 
     6781 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa 
     6841 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa 
     6901 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc 
     6961 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa 
     7021 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa 
     7081 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg 
     7141 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc 
     7201 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg 
     7261 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg 
     7321 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg 
     7381 aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc 
     7441 gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac 
     7501 tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga 
     7561 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc 
     7621 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagggtgc 
     7681 cttgatgtgg gcgccggcgg tcgagtggcg acggcgcggc ttgtccgcgc cctggtagat 
     7741 tgcctggccc taggccagcc atttttgagc ggccagcggc cgcgataggc cgacgcgaag 
     7801 cggcggggcg tagggagcgc agcgaccgaa gggtaggcgc tttttgcagc tcttcggctg 
     7861 tgcgctggcc agacagttat gcacaggcca ggcgggtttt aagagtttta ataagtttta 
     7921 aagagtttta ggcggaaaaa tcgccttttt tctcttttat atcagtcact tacatgtgtg 
     7981 accggttccc aatgtacggc tttgggttcc caatgtacgg gttccggttc ccaatgtacg 
     8041 gctttgggtt cccaatgtac gtgctatcca caggaaagag accttttcga cctttttccc 
     8101 ctgctagggc aatttgccct agcatctgct ccgtacatta ggaaccggcg gatgcttcgc 
     8161 cctcgatcag gttgcggtag cgcatgacta ggatcgggcc agcctgcccc gcctcctcct 
     8221 tcaaatcgta ctccggcagg tcatttgacc cgatcagctt gcgcacggtg aaacagaact 
     8281 tcttgaactc tccggcgctg ccactgcgtt cgtagatcgt cttgaacaac catctggctt 
     8341 ctgccttgcc tgcggcgcgg cgtgccaggc ggtagagaaa acggccgatg ccgggatcga 
     8401 tcaaaaagta atcggggtga accgtcagca cgtccgggtt cttgccttct gtgatctcgc 
     8461 ggtacatcca atcagctagc tcgatctcga tgtactccgg ccgcccggtt tcgctcttta 
     8521 cgatcttgta gcggctaatc aaggcttcac cctcggatac cgtcaccagg cggccgttct 
     8581 tggccttctt cgtacgctgc atggcaacgt gcgtggtgtt taaccgaatg caggtttcta 
     8641 ccaggtcgtc tttctgcttt ccgccatcgg ctcgccggca gaacttgagt acgtccgcaa 
     8701 cgtgtggacg gaacacgcgg ccgggcttgt ctcccttccc ttcccggtat cggttcatgg 
     8761 attcggttag atgggaaacc gccatcagta ccaggtcgta atcccacaca ctggccatgc 
     8821 cggccggccc tgcggaaacc tctacgtgcc cgtctggaag ctcgtagcgg atcacctcgc 
     8881 cagctcgtcg gtcacgcttc gacagacgga aaacggccac gtccatgatg ctgcgactat 
     8941 cgcgggtgcc cacgtcatag agcatcggaa cgaaaaaatc tggttgctcg tcgcccttgg 
     9001 gcggcttcct aatcgacggc gcaccggctg ccggcggttg ccgggattct ttgcggattc 
     9061 gatcagcggc cgcttgccac gattcaccgg ggcgtgcttc tgcctcgatg cgttgccgct 
     9121 gggcggcctg cgcggccttc aacttctcca ccaggtcatc acccagcgcc gcgccgattt 
     9181 gtaccgggcc ggatggtttg cgaccgtcac gccgattcct cgggcttggg ggttccagtg 
     9241 ccattgcagg gccggcagac aacccagccg cttacgcctg gccaaccgcc cgttcctcca 
     9301 cacatggggc attccacggc gtcggtgcct ggttgttctt gattttccat gccgcctcct 
     9361 ttagccgcta aaattcatct actcatttat tcatttgctc atttactctg gtagctgcgc 
     9421 gatgtattca gatagcagct cggtaatggt cttgccttgg cgtaccgcgt acatcttcag 
     9481 cttggtgtga tcctccgccg gcaactgaaa gttgacccgc ttcatggctg gcgtgtctgc 
     9541 caggctggcc aacgttgcag ccttgctgct gcgtgcgctc ggacggccgg cacttagcgt 
     9601 gtttgtgctt ttgctcattt tctctttacc tcattaactc aaatgagttt tgatttaatt 
     9661 tcagcggcca gcgcctggac ctcgcgggca gcgtcgccct cgggttctga ttcaagaacg 
     9721 gttgtgccgg cggcggcagt gcctgggtag ctcacgcgct gcgtgatacg ggactcaaga 
     9781 atgggcagct cgtacccggc cagcgcctcg gcaacctcac cgccgatgcg cgtgcctttg 
     9841 atcgcccgcg acacgacaaa ggccgcttgt agccttccat ccgtgacctc aatgcgctgc 
     9901 ttaaccagct ccaccaggtc ggcggtggcc catatgtcgt aagggcttgg ctgcaccgga 
     9961 atcagcacga agtcggctgc cttgatcgcg gacacagcca agtccgccgc ctggggcgct 
    10021 ccgtcgatca ctacgaagtc gcgccggccg atggccttca cgtcgcggtc aatcgtcggg 
    10081 cggtcgatgc cgacaacggt tagcggttga tcttcccgca cggccgccca atcgcgggca 
    10141 ctgccctggg gatcggaatc gactaacaga acatcggccc cggcgagttg cagggcgcgg 
    10201 gctagatggg ttgcgatggt cgtcttgcct gacccgcctt tctggttaag tacagcgata 
    10261 accttcatgc gttccccttg cgtatttgtt tatttactca tcgcatcata tacgcagcga 
    10321 ccgcatgacg caagctgttt tactcaaata cacatcacct ttttagacgg cggcgctcgg 
    10381 tttcttcagc ggccaagctg gccggccagg ccgccagctt ggcatcagac aaaccggcca 
    10441 ggatttcatg cagccgcacg gttgagacgt gcgcgggcgg ctcgaacacg tacccggccg 
    10501 cgatcatctc cgcctcgatc tcttcggtaa tgaaaaacgg ttcgtcctgg ccgtcctggt 
    10561 gcggtttcat gcttgttcct cttggcgttc attctcggcg gccgccaggg cgtcggcctc 
    10621 ggtcaatgcg tcctcacgga aggcaccgcg ccgcctggcc tcggtgggcg tcacttcctc 
    10681 gctgcgctca agtgcgcggt acagggtcga gcgatgcacg ccaagcagtg cagccgcctc 
    10741 tttcacggtg cggccttcct ggtcgatcag ctcgcgggcg tgcgcgatct gtgccggggt 
    10801 gagggtaggg cgggggccaa acttcacgcc tcgggccttg gcggcctcgc gcccgctccg 
    10861 ggtgcggtcg atgattaggg aacgctcgaa ctcggcaatg ccggcgaaca cggtcaacac 
    10921 catgcggccg gccggcgtgg tggtgtcggc ccacggctct gccaggctac gcaggcccgc 
    10981 gccggcctcc tggatgcgct cggcaatgtc cagtaggtcg cgggtgctgc gggccaggcg 
    11041 gtctagcctg gtcactgtca caacgtcgcc agggcgtagg tggtcaagca tcctggccag 
    11101 ctccgggcgg tcgcgcctgg tgccggtgat cttctcggaa aacagcttgg tgcagccggc 
    11161 cgcgtgcagt tcggcccgtt ggttggtcaa gtcctggtcg tcggtgctga cgcgggcata 
    11221 gcccagcagg ccagcggcgg cgctcttgtt catggcgtaa tgtctccggt tctagtcgca 
    11281 agtattctac tttatgcgac taaaacacgc gacaagaaaa cgccaggaaa agggcagggc 
    11341 ggcagcctgt cgcgtaactt aggacttgtg cgacatgtcg ttttcagaag acggctgcac 
    11401 tgaacgtcag aagccgactg cactatagca gcggaggggt tggatcaaag tactttaaag 
    11461 tactttaaag tactttaaag tactttgatc ccgaggggaa ccctgtggtt ggcatgcaca 
    11521 tacaaatgga cgaacggata aaccttttca cgccctttta aatatccgtt attctaataa 
    11581 acgctctttt ctcttaggtt tacccgccaa tatatcctgt caaacactga tagtttaaac 
    11641 tgaaggcggg aaacgacaat ctgatccaag ctcaagctgc tctagccaat acgcaaaccg 
    11701 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg 
    11761 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag 
    11821 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt 
    11881 cacacaggaa acagctatga ccatgattac gaattcgagc tcgg 
//
