LOCUS       pUBC-cYFP-DEST       11073 bp    DNA     circular     30-SEP-2010
SOURCE      
  ORGANISM  
REFERENCE   1  (bases 1 to 10818)
  AUTHORS   Grefen,C., Donald,N., Hashimoto,K., Kudla,J., Schumacher,K. and
            Blatt,M.R.
  TITLE     A ubiquitin-10 promoter-based vector set for fluorescent protein
            tagging facilitates temporal stability and native protein
            distribution in transient and stable expression studies
  JOURNAL   Plant J. 64 (2), 355-365 (2010)
   PUBMED   20735773
FEATURES             Location/Qualifiers
     CDS             901..1560
                     /label=CmR
     misc_recomb     668..792
                     /label=attR1
     misc_recomb     complement(2248..2372)
                     /label=attR2
     CDS             1902..2207
                     /label=ccdB
     misc_feature    complement(4116..4448)
                     /label=LB
     misc_feature    10633..10781
                     /label=RB
     terminator      2704..2912
                     /label=T35S
     CDS             2962..4112
                     /label=BarR
     promoter        1..639
                     /label=pUBQ10
     CDS             4512..5522
                     /label=SpecR
     exon            2425..2679
                     /label=cYFP
     rep_origin      5973..6653
                     /label=pBR322
     rep_origin      7063..9656
                     /label=pVS1
BASE COUNT     2495 a      2954 c      2908 g      2716 t 
ORIGIN
        1 tacccgacga gtcagtaata aacggcgtca aagtggttgc agccggcaca cacgagtcgt 
       61 gtttatcaac tcaaagcaca aatacttttc ctcaacctaa aaataaggca attagccaaa 
      121 aacaactttg cgtgtaaaca acgctcaata cacgtgtcat tttattatta gctattgctt 
      181 caccgcctta gctttctcgt gacctagtcg tcctcgtctt ttcttcttct tcttctataa 
      241 aacaataccc aaagagctct tcttcttcac aattcagatt tcaatttctc aaaatcttaa 
      301 aaactttctc tcaattctct ctaccgtgat caaggtaaat ttctgtgttc cttattctct 
      361 caaaatcttc gattttgttt tcgttcgatc ccaatttcgt atatgttctt tggtttagat 
      421 tctgttaatc ttagatcgaa gacgattttc tgggtttgat cgttagatat catcttaatt 
      481 ctcgattagg gtttcataga tatcatccga tttgttcaaa taatttgagt tttgtcgaat 
      541 aattactctt cgatttgtga tttctatcta gatctggtgt tagtttctag tttgtgcgat 
      601 cgaatttgta gattaatctg agtttttctg attaacactc gagtgcggga tcctctaagg 
      661 gcccatcaca agtttgtaca aaaaagctga acgagaaacg taaaatgata taaatatcaa 
      721 tatattaaat tagattttgc ataaaaaaca gactacataa tactgtaaaa cacaacatat 
      781 ccagtcacta tggcggccgc attaggcacc ccaggcttta cactttatgc ttccggctcg 
      841 tataatgtgt ggattttgag ttaggatccg tcgagatttt caggagctaa ggaagctaaa 
      901 atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa 
      961 cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat 
     1021 attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc ggcctttatt 
     1081 cacattcttg cccgcctgat gaatgctcat ccggaattcc gtatggcaat gaaagacggt 
     1141 gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga gcaaactgaa 
     1201 acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct acacatatat 
     1261 tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg gtttattgag 
     1321 aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga tttaaacgtg 
     1381 gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc 
     1441 gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga tggcttccat 
     1501 gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggg cggggcgtaa 
     1561 acgcgtggat ccggcttact aaaagccaga taacagtatg cgtatttgcg cgctgatttt 
     1621 tgcggtataa gaatatatac tgatatgtat acccgaagta tgtcaaaaag aggtatgcta 
     1681 tgaagcagcg tattacagtg acagttgaca gcgacagcta tcagttgctc aaggcatata 
     1741 tgatgtcaat atctccggtc tggtaagcac aaccatgcag aatgaagccc gtcgtctgcg 
     1801 tgccgaacgc tggaaagcgg aaaatcagga agggatggct gaggtcgccc ggtttattga 
     1861 aatgaacggc tcttttgctg acgagaacag gggctggtga aatgcagttt aaggtttaca 
     1921 cctataaaag agagagccgt tatcgtctgt ttgtggatgt acagagtgat attattgaca 
     1981 cgcccgggcg acggatggtg atccccctgg ccagtgcacg tctgctgtca gataaagtct 
     2041 cccgtgaact ttacccggtg gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg 
     2101 atatggccag tgtgccggtc tccgttatcg gggaagaagt ggctgatctc agccaccgcg 
     2161 aaaatgacat caaaaacgcc attaacctga tgttctgggg aatataaatg tcaggctccc 
     2221 ttatacacag ccagtctgca ggtcgaccat agtgactgga tatgttgtgt tttacagtat 
     2281 tatgtagtct gttttttatg caaaatctaa tttaatatat tgatatttat atcattttac 
     2341 gtttctcgtt cagctttctt gtacaaagtg gtgatgggac gtccgcggag atctacgcgt 
     2401 gtcgactcga gatatccaac tagtatggac aagcagaaga acggcatcaa ggtgaacttc 
     2461 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac 
     2521 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag ctaccagtcc 
     2581 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc 
     2641 gccgccggga tcactctcgg catggacgag ctgtacaagt tataagcggc catgctagag 
     2701 tccgcaaaaa tcaccagtct ctctctacaa atctatctct ctctattttt ctccagaata 
     2761 atgtgtgagt agttcccaga taagggaatt agggttctta tagggtttcg ctcatgtgtt 
     2821 gagcatataa gaaaccctta gtatgtattt gtatttgtaa aatacttcta tcaataaaat 
     2881 ttctaattcc taaaaccaaa atccagtgac ctgcaggcat gcgacgtcgg gccctctaga 
     2941 ggatccccgg gtaccgcgaa ttatcgatca tgagcggaga attaagggag tcacgttatg 
     3001 acccccgccg atgacgcggg acaagccgtt ttacgtttgg aactgacaga accgcaacgt 
     3061 tgaaggagcc actgagccgc gggtttctgg agtttaatga gctaagcaca tacgtcagaa 
     3121 accattattg cgcgttcaaa agtcgcctaa ggtcactatc agctagcaaa tatttcttgt 
     3181 caaaaatgct ccactgacgt tccataaatt cccctcggta tccaattaga gtctcatatt 
     3241 cactctcaac tcgatcgagg ggatctacca tgagcccaga acgacgcccg gccgacatcc 
     3301 gccgtgccac cgaggcggac atgccggcgg tctgcaccat cgtcaaccac tacatcgaga 
     3361 caagcacggt caacttccgt accgagccgc aggaaccgca ggagtggacg gacgacctcg 
     3421 tccgtctgcg ggagcgctat ccctggctcg tcgccgaggt ggacggcgag gtcgccggca 
     3481 tcgcctacgc gggtccctgg aaggcacgca acgcctacga ctggacggcc gagtcgaccg 
     3541 tgtacgtctc cccccgccac cagcggacgg gactgggctc cacgctctac acccacctgc 
     3601 tgaagtccct ggaggcacag ggcttcaaga gcgtggtcgc tgtcatcggg ctgcccaacg 
     3661 acccgagcgt gcgcatgcac gaggcgctcg gatatgcccc ccgcggcatg ctgcgggcgg 
     3721 ccggcttcaa gcacgggaac tggcatgacg tgggtttctg gcagctggac ttcagcctgc 
     3781 cggtgccgcc ccgtccggtc ctgcccgtca ccgaaatctg atgaccccta gagtcaagca 
     3841 gatcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc cggtcttgcg 
     3901 atgattatca tataatttct gttgaattac gttaagcatg taataattaa catgtaatgc 
     3961 atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata catttaatac 
     4021 gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct 
     4081 atgttactag atcgaccggc atgcaagctg ataattcaat tcggcgttaa ttcagtacat 
     4141 taaaaacgtc cgcaatgtgt tattaagttg tctaagcgtc aatttgttta caccacaata 
     4201 tatcctgcca ccagccagcc aacagctccc cgaccggcag ctcggcacaa aatcaccact 
     4261 cgatacaggc agcccatcag tccgggacgg cgtcagcggg agagccgttg taaggcggca 
     4321 gactttgctc atgttaccga tgctattcgg aagaacggca actaagctgc cgggtttgaa 
     4381 acacggatga tctcgcggag ggtagcatgt tgattgtaac gatgacagag cgttgctgcc 
     4441 tgtgatcaat tcgggcacga acccagtgga cataagcctg ttcggttcgt aagctgtaat 
     4501 gcaagtagcg tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta 
     4561 acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttggggta cagtctatgc 
     4621 ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca 
     4681 gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc atgggggaag 
     4741 cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc 
     4801 tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc 
     4861 cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc 
     4921 gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc 
     4981 gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta 
     5041 agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc 
     5101 cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg 
     5161 ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg 
     5221 aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc 
     5281 gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc 
     5341 cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca 
     5401 tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag 
     5461 atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta gtcggcaaat 
     5521 aatgtctagc tagaaattcg ttcaagccga cgccgcttcg cggcgcggct taactcaagt 
     5581 cgttagatgc actaagcaca taattgctca cagccaaact atcaggtcaa gtctgctttt 
     5641 attattttta agcgtgcata ataagcccta cacaaattgg gagatatatc atgcatgacc 
     5701 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa 
     5761 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca 
     5821 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta 
     5881 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc 
     5941 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca 
     6001 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta 
     6061 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag 
     6121 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt 
     6181 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc 
     6241 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac 
     6301 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac 
     6361 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc 
     6421 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat 
     6481 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag 
     6541 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc 
     6601 actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc 
     6661 tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac 
     6721 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca 
     6781 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagggtgcc ttgatgtggg 
     6841 cgccggcggt cgagtggcga cggcgcggct tgtccgcgcc ctggtagatt gcctggccct 
     6901 aggccagcca tttttgagcg gccagcggcc gcgataggcc gacgcgaagc ggcggggcgt 
     6961 agggagcgca gcgaccgaag ggtaggcgct ttttgcagct cttcggctgt gcgctggcca 
     7021 gacagttatg cacaggccag gcgggtttta agagttttaa taagttttaa agagttttag 
     7081 gcggaaaaat cgcctttttt ctcttttata tcagtcactt acatgtgtga ccggttccca 
     7141 atgtacggct ttgggttccc aatgtacggg ttccggttcc caatgtacgg ctttgggttc 
     7201 ccaatgtacg tgctatccac aggaaagaga ccttttcgac ctttttcccc tgctagggca 
     7261 atttgcccta gcatctgctc cgtacattag gaaccggcgg atgcttcgcc ctcgatcagg 
     7321 ttgcggtagc gcatgactag gatcgggcca gcctgccccg cctcctcctt caaatcgtac 
     7381 tccggcaggt catttgaccc gatcagcttg cgcacggtga aacagaactt cttgaactct 
     7441 ccggcgctgc cactgcgttc gtagatcgtc ttgaacaacc atctggcttc tgccttgcct 
     7501 gcggcgcggc gtgccaggcg gtagagaaaa cggccgatgc cgggatcgat caaaaagtaa 
     7561 tcggggtgaa ccgtcagcac gtccgggttc ttgccttctg tgatctcgcg gtacatccaa 
     7621 tcagctagct cgatctcgat gtactccggc cgcccggttt cgctctttac gatcttgtag 
     7681 cggctaatca aggcttcacc ctcggatacc gtcaccaggc ggccgttctt ggccttcttc 
     7741 gtacgctgca tggcaacgtg cgtggtgttt aaccgaatgc aggtttctac caggtcgtct 
     7801 ttctgctttc cgccatcggc tcgccggcag aacttgagta cgtccgcaac gtgtggacgg 
     7861 aacacgcggc cgggcttgtc tcccttccct tcccggtatc ggttcatgga ttcggttaga 
     7921 tgggaaaccg ccatcagtac caggtcgtaa tcccacacac tggccatgcc ggccggccct 
     7981 gcggaaacct ctacgtgccc gtctggaagc tcgtagcgga tcacctcgcc agctcgtcgg 
     8041 tcacgcttcg acagacggaa aacggccacg tccatgatgc tgcgactatc gcgggtgccc 
     8101 acgtcataga gcatcggaac gaaaaaatct ggttgctcgt cgcccttggg cggcttccta 
     8161 atcgacggcg caccggctgc cggcggttgc cgggattctt tgcggattcg atcagcggcc 
     8221 gcttgccacg attcaccggg gcgtgcttct gcctcgatgc gttgccgctg ggcggcctgc 
     8281 gcggccttca acttctccac caggtcatca cccagcgccg cgccgatttg taccgggccg 
     8341 gatggtttgc gaccgtcacg ccgattcctc gggcttgggg gttccagtgc cattgcaggg 
     8401 ccggcagaca acccagccgc ttacgcctgg ccaaccgccc gttcctccac acatggggca 
     8461 ttccacggcg tcggtgcctg gttgttcttg attttccatg ccgcctcctt tagccgctaa 
     8521 aattcatcta ctcatttatt catttgctca tttactctgg tagctgcgcg atgtattcag 
     8581 atagcagctc ggtaatggtc ttgccttggc gtaccgcgta catcttcagc ttggtgtgat 
     8641 cctccgccgg caactgaaag ttgacccgct tcatggctgg cgtgtctgcc aggctggcca 
     8701 acgttgcagc cttgctgctg cgtgcgctcg gacggccggc acttagcgtg tttgtgcttt 
     8761 tgctcatttt ctctttacct cattaactca aatgagtttt gatttaattt cagcggccag 
     8821 cgcctggacc tcgcgggcag cgtcgccctc gggttctgat tcaagaacgg ttgtgccggc 
     8881 ggcggcagtg cctgggtagc tcacgcgctg cgtgatacgg gactcaagaa tgggcagctc 
     8941 gtacccggcc agcgcctcgg caacctcacc gccgatgcgc gtgcctttga tcgcccgcga 
     9001 cacgacaaag gccgcttgta gccttccatc cgtgacctca atgcgctgct taaccagctc 
     9061 caccaggtcg gcggtggccc atatgtcgta agggcttggc tgcaccggaa tcagcacgaa 
     9121 gtcggctgcc ttgatcgcgg acacagccaa gtccgccgcc tggggcgctc cgtcgatcac 
     9181 tacgaagtcg cgccggccga tggccttcac gtcgcggtca atcgtcgggc ggtcgatgcc 
     9241 gacaacggtt agcggttgat cttcccgcac ggccgcccaa tcgcgggcac tgccctgggg 
     9301 atcggaatcg actaacagaa catcggcccc ggcgagttgc agggcgcggg ctagatgggt 
     9361 tgcgatggtc gtcttgcctg acccgccttt ctggttaagt acagcgataa ccttcatgcg 
     9421 ttccccttgc gtatttgttt atttactcat cgcatcatat acgcagcgac cgcatgacgc 
     9481 aagctgtttt actcaaatac acatcacctt tttagacggc ggcgctcggt ttcttcagcg 
     9541 gccaagctgg ccggccaggc cgccagcttg gcatcagaca aaccggccag gatttcatgc 
     9601 agccgcacgg ttgagacgtg cgcgggcggc tcgaacacgt acccggccgc gatcatctcc 
     9661 gcctcgatct cttcggtaat gaaaaacggt tcgtcctggc cgtcctggtg cggtttcatg 
     9721 cttgttcctc ttggcgttca ttctcggcgg ccgccagggc gtcggcctcg gtcaatgcgt 
     9781 cctcacggaa ggcaccgcgc cgcctggcct cggtgggcgt cacttcctcg ctgcgctcaa 
     9841 gtgcgcggta cagggtcgag cgatgcacgc caagcagtgc agccgcctct ttcacggtgc 
     9901 ggccttcctg gtcgatcagc tcgcgggcgt gcgcgatctg tgccggggtg agggtagggc 
     9961 gggggccaaa cttcacgcct cgggccttgg cggcctcgcg cccgctccgg gtgcggtcga 
    10021 tgattaggga acgctcgaac tcggcaatgc cggcgaacac ggtcaacacc atgcggccgg 
    10081 ccggcgtggt ggtgtcggcc cacggctctg ccaggctacg caggcccgcg ccggcctcct 
    10141 ggatgcgctc ggcaatgtcc agtaggtcgc gggtgctgcg ggccaggcgg tctagcctgg 
    10201 tcactgtcac aacgtcgcca gggcgtaggt ggtcaagcat cctggccagc tccgggcggt 
    10261 cgcgcctggt gccggtgatc ttctcggaaa acagcttggt gcagccggcc gcgtgcagtt 
    10321 cggcccgttg gttggtcaag tcctggtcgt cggtgctgac gcgggcatag cccagcaggc 
    10381 cagcggcggc gctcttgttc atggcgtaat gtctccggtt ctagtcgcaa gtattctact 
    10441 ttatgcgact aaaacacgcg acaagaaaac gccaggaaaa gggcagggcg gcagcctgtc 
    10501 gcgtaactta ggacttgtgc gacatgtcgt tttcagaaga cggctgcact gaacgtcaga 
    10561 agccgactgc actatagcag cggaggggtt ggatcaaagt actttaaagt actttaaagt 
    10621 actttaaagt actttgatcc cgaggggaac cctgtggttg gcatgcacat acaaatggac 
    10681 gaacggataa accttttcac gcccttttaa atatccgtta ttctaataaa cgctcttttc 
    10741 tcttaggttt acccgccaat atatcctgtc aaacactgat agtttaaact gaaggcggga 
    10801 aacgacaatc tgatccaagc tcaagctgct ctagccaata cgcaaaccgc ctctccccgc 
    10861 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag 
    10921 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt 
    10981 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa 
    11041 cagctatgac catgattacg aattcgagct cgg 
//
