LOCUS       pUBC-CFP-DEST       11538 bp    DNA     circular     30-SEP-2010
SOURCE      
  ORGANISM  
REFERENCE   
  AUTHORS   Grefen,C., Donald,N., Hashimoto,K., Kudla,J., Schumacher,K. and
            Blatt,M.R.
  TITLE     A ubiquitin-10 promoter-based vector set for fluorescent protein
            tagging facilitates temporal stability and native protein
            distribution in transient and stable expression studies
  JOURNAL   Plant J. 64 (2), 355-365 (2010)
   PUBMED   20735773
FEATURES             Location/Qualifiers
     CDS             901..1560
                     /label=CmR
     misc_recomb     668..792
                     /label=attR1
     misc_recomb     complement(2248..2372)
                     /label=attR2
     CDS             1902..2207
                     /label=ccdB
     misc_feature    complement(4581..4913)
                     /label=LB
     misc_feature    11098..11246
                     /label=RB
     terminator      3169..3377
                     /label=T35S
     CDS             3427..4577
                     /label=BarR
     promoter        1..639
                     /label=pUBQ10
     CDS             4977..5987
                     /label=SpecR
     exon            2425..3141
                     /label=CFP
     rep_origin      7528..10121
                     /label=pVS1
     rep_origin      6438..7118
                     /label=pBR322
BASE COUNT     2601 a      3109 c      3043 g      2785 t 
ORIGIN
        1 tacccgacga gtcagtaata aacggcgtca aagtggttgc agccggcaca cacgagtcgt 
       61 gtttatcaac tcaaagcaca aatacttttc ctcaacctaa aaataaggca attagccaaa 
      121 aacaactttg cgtgtaaaca acgctcaata cacgtgtcat tttattatta gctattgctt 
      181 caccgcctta gctttctcgt gacctagtcg tcctcgtctt ttcttcttct tcttctataa 
      241 aacaataccc aaagagctct tcttcttcac aattcagatt tcaatttctc aaaatcttaa 
      301 aaactttctc tcaattctct ctaccgtgat caaggtaaat ttctgtgttc cttattctct 
      361 caaaatcttc gattttgttt tcgttcgatc ccaatttcgt atatgttctt tggtttagat 
      421 tctgttaatc ttagatcgaa gacgattttc tgggtttgat cgttagatat catcttaatt 
      481 ctcgattagg gtttcataga tatcatccga tttgttcaaa taatttgagt tttgtcgaat 
      541 aattactctt cgatttgtga tttctatcta gatctggtgt tagtttctag tttgtgcgat 
      601 cgaatttgta gattaatctg agtttttctg attaacactc gagtgcggga tcctctaagg 
      661 gcccatcaca agtttgtaca aaaaagctga acgagaaacg taaaatgata taaatatcaa 
      721 tatattaaat tagattttgc ataaaaaaca gactacataa tactgtaaaa cacaacatat 
      781 ccagtcacta tggcggccgc attaggcacc ccaggcttta cactttatgc ttccggctcg 
      841 tataatgtgt ggattttgag ttaggatccg tcgagatttt caggagctaa ggaagctaaa 
      901 atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa 
      961 cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat 
     1021 attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc ggcctttatt 
     1081 cacattcttg cccgcctgat gaatgctcat ccggaattcc gtatggcaat gaaagacggt 
     1141 gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga gcaaactgaa 
     1201 acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct acacatatat 
     1261 tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg gtttattgag 
     1321 aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga tttaaacgtg 
     1381 gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc 
     1441 gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga tggcttccat 
     1501 gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggg cggggcgtaa 
     1561 acgcgtggat ccggcttact aaaagccaga taacagtatg cgtatttgcg cgctgatttt 
     1621 tgcggtataa gaatatatac tgatatgtat acccgaagta tgtcaaaaag aggtatgcta 
     1681 tgaagcagcg tattacagtg acagttgaca gcgacagcta tcagttgctc aaggcatata 
     1741 tgatgtcaat atctccggtc tggtaagcac aaccatgcag aatgaagccc gtcgtctgcg 
     1801 tgccgaacgc tggaaagcgg aaaatcagga agggatggct gaggtcgccc ggtttattga 
     1861 aatgaacggc tcttttgctg acgagaacag gggctggtga aatgcagttt aaggtttaca 
     1921 cctataaaag agagagccgt tatcgtctgt ttgtggatgt acagagtgat attattgaca 
     1981 cgcccgggcg acggatggtg atccccctgg ccagtgcacg tctgctgtca gataaagtct 
     2041 cccgtgaact ttacccggtg gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg 
     2101 atatggccag tgtgccggtc tccgttatcg gggaagaagt ggctgatctc agccaccgcg 
     2161 aaaatgacat caaaaacgcc attaacctga tgttctgggg aatataaatg tcaggctccc 
     2221 ttatacacag ccagtctgca ggtcgaccat agtgactgga tatgttgtgt tttacagtat 
     2281 tatgtagtct gttttttatg caaaatctaa tttaatatat tgatatttat atcattttac 
     2341 gtttctcgtt cagctttctt gtacaaagtg gtgatgggac gtccgcggag atctacgcgt 
     2401 gtcgactcga gatatccaac tagtatggtg agcaagggcg aggagctgtt caccggggtg 
     2461 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc 
     2521 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc 
     2581 aagctgcccg tgccctggcc caccctcgtg accaccctga cctggggcgt gcagtgcttc 
     2641 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc 
     2701 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag 
     2761 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag 
     2821 gaggacggca acatcctggg gcacaagctg gagtacaact acatcagcca caacgtctat 
     2881 atcaccgccg acaagcagaa gaacggcatc aaggccaact tcaagatccg ccacaacatc 
     2941 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc 
     3001 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc 
     3061 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc 
     3121 ggcatggacg agctgtacaa gtaattataa gcggccatgc tagagtccgc aaaaatcacc 
     3181 agtctctctc tacaaatcta tctctctcta tttttctcca gaataatgtg tgagtagttc 
     3241 ccagataagg gaattagggt tcttataggg tttcgctcat gtgttgagca tataagaaac 
     3301 ccttagtatg tatttgtatt tgtaaaatac ttctatcaat aaaatttcta attcctaaaa 
     3361 ccaaaatcca gtgacctgca ggcatgcgac gtcgggccct ctagaggatc cccgggtacc 
     3421 gcgaattatc gatcatgagc ggagaattaa gggagtcacg ttatgacccc cgccgatgac 
     3481 gcgggacaag ccgttttacg tttggaactg acagaaccgc aacgttgaag gagccactga 
     3541 gccgcgggtt tctggagttt aatgagctaa gcacatacgt cagaaaccat tattgcgcgt 
     3601 tcaaaagtcg cctaaggtca ctatcagcta gcaaatattt cttgtcaaaa atgctccact 
     3661 gacgttccat aaattcccct cggtatccaa ttagagtctc atattcactc tcaactcgat 
     3721 cgaggggatc taccatgagc ccagaacgac gcccggccga catccgccgt gccaccgagg 
     3781 cggacatgcc ggcggtctgc accatcgtca accactacat cgagacaagc acggtcaact 
     3841 tccgtaccga gccgcaggaa ccgcaggagt ggacggacga cctcgtccgt ctgcgggagc 
     3901 gctatccctg gctcgtcgcc gaggtggacg gcgaggtcgc cggcatcgcc tacgcgggtc 
     3961 cctggaaggc acgcaacgcc tacgactgga cggccgagtc gaccgtgtac gtctcccccc 
     4021 gccaccagcg gacgggactg ggctccacgc tctacaccca cctgctgaag tccctggagg 
     4081 cacagggctt caagagcgtg gtcgctgtca tcgggctgcc caacgacccg agcgtgcgca 
     4141 tgcacgaggc gctcggatat gccccccgcg gcatgctgcg ggcggccggc ttcaagcacg 
     4201 ggaactggca tgacgtgggt ttctggcagc tggacttcag cctgccggtg ccgccccgtc 
     4261 cggtcctgcc cgtcaccgaa atctgatgac ccctagagtc aagcagatcg ttcaaacatt 
     4321 tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa 
     4381 tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg 
     4441 agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa 
     4501 atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt actagatcga 
     4561 ccggcatgca agctgataat tcaattcggc gttaattcag tacattaaaa acgtccgcaa 
     4621 tgtgttatta agttgtctaa gcgtcaattt gtttacacca caatatatcc tgccaccagc 
     4681 cagccaacag ctccccgacc ggcagctcgg cacaaaatca ccactcgata caggcagccc 
     4741 atcagtccgg gacggcgtca gcgggagagc cgttgtaagg cggcagactt tgctcatgtt 
     4801 accgatgcta ttcggaagaa cggcaactaa gctgccgggt ttgaaacacg gatgatctcg 
     4861 cggagggtag catgttgatt gtaacgatga cagagcgttg ctgcctgtga tcaattcggg 
     4921 cacgaaccca gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc 
     4981 gctcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg 
     5041 ttttcatggc ttgttatgac tgtttttttg gggtacagtc tatgcctcgg gcatccaagc 
     5101 agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc 
     5161 agcagggcag tcgccctaaa acaaagttaa acatcatggg ggaagcggtg atcgccgaag 
     5221 tatcgactca actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc 
     5281 tggccgtaca tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg 
     5341 atttgctggt tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg 
     5401 accttttgga aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca 
     5461 ccattgttgt gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat 
     5521 ttggagaatg gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca 
     5581 ttgatctggc tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag 
     5641 cggcggagga actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa 
     5701 ccttaacgct atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta 
     5761 cgttgtcccg catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg 
     5821 ccgactgggc aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac 
     5881 aggcttatct tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat 
     5941 ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg caaataatgt ctagctagaa 
     6001 attcgttcaa gccgacgccg cttcgcggcg cggcttaact caagtcgtta gatgcactaa 
     6061 gcacataatt gctcacagcc aaactatcag gtcaagtctg cttttattat ttttaagcgt 
     6121 gcataataag ccctacacaa attgggagat atatcatgca tgaccaaaat cccttaacgt 
     6181 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat 
     6241 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg 
     6301 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga 
     6361 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac 
     6421 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt 
     6481 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag 
     6541 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc 
     6601 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag 
     6661 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca 
     6721 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt 
     6781 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc 
     6841 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc 
     6901 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc 
     6961 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat 
     7021 tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc 
     7081 tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca 
     7141 tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc 
     7201 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt 
     7261 caccgtcatc accgaaacgc gcgaggcagg gtgccttgat gtgggcgccg gcggtcgagt 
     7321 ggcgacggcg cggcttgtcc gcgccctggt agattgcctg gccctaggcc agccattttt 
     7381 gagcggccag cggccgcgat aggccgacgc gaagcggcgg ggcgtaggga gcgcagcgac 
     7441 cgaagggtag gcgctttttg cagctcttcg gctgtgcgct ggccagacag ttatgcacag 
     7501 gccaggcggg ttttaagagt tttaataagt tttaaagagt tttaggcgga aaaatcgcct 
     7561 tttttctctt ttatatcagt cacttacatg tgtgaccggt tcccaatgta cggctttggg 
     7621 ttcccaatgt acgggttccg gttcccaatg tacggctttg ggttcccaat gtacgtgcta 
     7681 tccacaggaa agagaccttt tcgacctttt tcccctgcta gggcaatttg ccctagcatc 
     7741 tgctccgtac attaggaacc ggcggatgct tcgccctcga tcaggttgcg gtagcgcatg 
     7801 actaggatcg ggccagcctg ccccgcctcc tccttcaaat cgtactccgg caggtcattt 
     7861 gacccgatca gcttgcgcac ggtgaaacag aacttcttga actctccggc gctgccactg 
     7921 cgttcgtaga tcgtcttgaa caaccatctg gcttctgcct tgcctgcggc gcggcgtgcc 
     7981 aggcggtaga gaaaacggcc gatgccggga tcgatcaaaa agtaatcggg gtgaaccgtc 
     8041 agcacgtccg ggttcttgcc ttctgtgatc tcgcggtaca tccaatcagc tagctcgatc 
     8101 tcgatgtact ccggccgccc ggtttcgctc tttacgatct tgtagcggct aatcaaggct 
     8161 tcaccctcgg ataccgtcac caggcggccg ttcttggcct tcttcgtacg ctgcatggca 
     8221 acgtgcgtgg tgtttaaccg aatgcaggtt tctaccaggt cgtctttctg ctttccgcca 
     8281 tcggctcgcc ggcagaactt gagtacgtcc gcaacgtgtg gacggaacac gcggccgggc 
     8341 ttgtctccct tcccttcccg gtatcggttc atggattcgg ttagatggga aaccgccatc 
     8401 agtaccaggt cgtaatccca cacactggcc atgccggccg gccctgcgga aacctctacg 
     8461 tgcccgtctg gaagctcgta gcggatcacc tcgccagctc gtcggtcacg cttcgacaga 
     8521 cggaaaacgg ccacgtccat gatgctgcga ctatcgcggg tgcccacgtc atagagcatc 
     8581 ggaacgaaaa aatctggttg ctcgtcgccc ttgggcggct tcctaatcga cggcgcaccg 
     8641 gctgccggcg gttgccggga ttctttgcgg attcgatcag cggccgcttg ccacgattca 
     8701 ccggggcgtg cttctgcctc gatgcgttgc cgctgggcgg cctgcgcggc cttcaacttc 
     8761 tccaccaggt catcacccag cgccgcgccg atttgtaccg ggccggatgg tttgcgaccg 
     8821 tcacgccgat tcctcgggct tgggggttcc agtgccattg cagggccggc agacaaccca 
     8881 gccgcttacg cctggccaac cgcccgttcc tccacacatg gggcattcca cggcgtcggt 
     8941 gcctggttgt tcttgatttt ccatgccgcc tcctttagcc gctaaaattc atctactcat 
     9001 ttattcattt gctcatttac tctggtagct gcgcgatgta ttcagatagc agctcggtaa 
     9061 tggtcttgcc ttggcgtacc gcgtacatct tcagcttggt gtgatcctcc gccggcaact 
     9121 gaaagttgac ccgcttcatg gctggcgtgt ctgccaggct ggccaacgtt gcagccttgc 
     9181 tgctgcgtgc gctcggacgg ccggcactta gcgtgtttgt gcttttgctc attttctctt 
     9241 tacctcatta actcaaatga gttttgattt aatttcagcg gccagcgcct ggacctcgcg 
     9301 ggcagcgtcg ccctcgggtt ctgattcaag aacggttgtg ccggcggcgg cagtgcctgg 
     9361 gtagctcacg cgctgcgtga tacgggactc aagaatgggc agctcgtacc cggccagcgc 
     9421 ctcggcaacc tcaccgccga tgcgcgtgcc tttgatcgcc cgcgacacga caaaggccgc 
     9481 ttgtagcctt ccatccgtga cctcaatgcg ctgcttaacc agctccacca ggtcggcggt 
     9541 ggcccatatg tcgtaagggc ttggctgcac cggaatcagc acgaagtcgg ctgccttgat 
     9601 cgcggacaca gccaagtccg ccgcctgggg cgctccgtcg atcactacga agtcgcgccg 
     9661 gccgatggcc ttcacgtcgc ggtcaatcgt cgggcggtcg atgccgacaa cggttagcgg 
     9721 ttgatcttcc cgcacggccg cccaatcgcg ggcactgccc tggggatcgg aatcgactaa 
     9781 cagaacatcg gccccggcga gttgcagggc gcgggctaga tgggttgcga tggtcgtctt 
     9841 gcctgacccg cctttctggt taagtacagc gataaccttc atgcgttccc cttgcgtatt 
     9901 tgtttattta ctcatcgcat catatacgca gcgaccgcat gacgcaagct gttttactca 
     9961 aatacacatc acctttttag acggcggcgc tcggtttctt cagcggccaa gctggccggc 
    10021 caggccgcca gcttggcatc agacaaaccg gccaggattt catgcagccg cacggttgag 
    10081 acgtgcgcgg gcggctcgaa cacgtacccg gccgcgatca tctccgcctc gatctcttcg 
    10141 gtaatgaaaa acggttcgtc ctggccgtcc tggtgcggtt tcatgcttgt tcctcttggc 
    10201 gttcattctc ggcggccgcc agggcgtcgg cctcggtcaa tgcgtcctca cggaaggcac 
    10261 cgcgccgcct ggcctcggtg ggcgtcactt cctcgctgcg ctcaagtgcg cggtacaggg 
    10321 tcgagcgatg cacgccaagc agtgcagccg cctctttcac ggtgcggcct tcctggtcga 
    10381 tcagctcgcg ggcgtgcgcg atctgtgccg gggtgagggt agggcggggg ccaaacttca 
    10441 cgcctcgggc cttggcggcc tcgcgcccgc tccgggtgcg gtcgatgatt agggaacgct 
    10501 cgaactcggc aatgccggcg aacacggtca acaccatgcg gccggccggc gtggtggtgt 
    10561 cggcccacgg ctctgccagg ctacgcaggc ccgcgccggc ctcctggatg cgctcggcaa 
    10621 tgtccagtag gtcgcgggtg ctgcgggcca ggcggtctag cctggtcact gtcacaacgt 
    10681 cgccagggcg taggtggtca agcatcctgg ccagctccgg gcggtcgcgc ctggtgccgg 
    10741 tgatcttctc ggaaaacagc ttggtgcagc cggccgcgtg cagttcggcc cgttggttgg 
    10801 tcaagtcctg gtcgtcggtg ctgacgcggg catagcccag caggccagcg gcggcgctct 
    10861 tgttcatggc gtaatgtctc cggttctagt cgcaagtatt ctactttatg cgactaaaac 
    10921 acgcgacaag aaaacgccag gaaaagggca gggcggcagc ctgtcgcgta acttaggact 
    10981 tgtgcgacat gtcgttttca gaagacggct gcactgaacg tcagaagccg actgcactat 
    11041 agcagcggag gggttggatc aaagtacttt aaagtacttt aaagtacttt aaagtacttt 
    11101 gatcccgagg ggaaccctgt ggttggcatg cacatacaaa tggacgaacg gataaacctt 
    11161 ttcacgccct tttaaatatc cgttattcta ataaacgctc ttttctctta ggtttacccg 
    11221 ccaatatatc ctgtcaaaca ctgatagttt aaactgaagg cgggaaacga caatctgatc 
    11281 caagctcaag ctgctctagc caatacgcaa accgcctctc cccgcgcgtt ggccgattca 
    11341 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat 
    11401 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg 
    11461 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga 
    11521 ttacgaattc gagctcgg 
//
