LOCUS       pUB-DEST          10818 bp    DNA     circular     30-SEP-2010
SOURCE      
  ORGANISM  
REFERENCE   1  (bases 1 to 10818)
  AUTHORS   Grefen,C., Donald,N., Hashimoto,K., Kudla,J., Schumacher,K. and
            Blatt,M.R.
  TITLE     A ubiquitin-10 promoter-based vector set for fluorescent protein
            tagging facilitates temporal stability and native protein
            distribution in transient and stable expression studies
  JOURNAL   Plant J. 64 (2), 355-365 (2010)
   PUBMED   20735773
FEATURES             Location/Qualifiers
     CDS             901..1560
                     /label=CmR
     misc_recomb     668..792
                     /label=attR1
     misc_recomb     complement(2248..2372)
                     /label=attR2
     CDS             1902..2207
                     /label=ccdB
     misc_feature    complement(3861..4193)
                     /label=LB
     misc_feature    10378..10526
                     /label=RB
     terminator      2449..2657
                     /label=T35S
     CDS             2707..3857
                     /label=BarR
     promoter        1..639
                     /label=pUBQ10
     CDS             4257..5267
                     /label=SpecR
     rep_origin      6808..9401
                     /label=pVS1
     rep_origin      5718..6398
                     /label=pBR322
BASE COUNT     2429 a      2866 c      2840 g      2683 t 
ORIGIN
        1 tacccgacga gtcagtaata aacggcgtca aagtggttgc agccggcaca cacgagtcgt 
       61 gtttatcaac tcaaagcaca aatacttttc ctcaacctaa aaataaggca attagccaaa 
      121 aacaactttg cgtgtaaaca acgctcaata cacgtgtcat tttattatta gctattgctt 
      181 caccgcctta gctttctcgt gacctagtcg tcctcgtctt ttcttcttct tcttctataa 
      241 aacaataccc aaagagctct tcttcttcac aattcagatt tcaatttctc aaaatcttaa 
      301 aaactttctc tcaattctct ctaccgtgat caaggtaaat ttctgtgttc cttattctct 
      361 caaaatcttc gattttgttt tcgttcgatc ccaatttcgt atatgttctt tggtttagat 
      421 tctgttaatc ttagatcgaa gacgattttc tgggtttgat cgttagatat catcttaatt 
      481 ctcgattagg gtttcataga tatcatccga tttgttcaaa taatttgagt tttgtcgaat 
      541 aattactctt cgatttgtga tttctatcta gatctggtgt tagtttctag tttgtgcgat 
      601 cgaatttgta gattaatctg agtttttctg attaacactc gagtgcggga tcctctaagg 
      661 gcccatcaca agtttgtaca aaaaagctga acgagaaacg taaaatgata taaatatcaa 
      721 tatattaaat tagattttgc ataaaaaaca gactacataa tactgtaaaa cacaacatat 
      781 ccagtcacta tggcggccgc attaggcacc ccaggcttta cactttatgc ttccggctcg 
      841 tataatgtgt ggattttgag ttaggatccg tcgagatttt caggagctaa ggaagctaaa 
      901 atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa 
      961 cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat 
     1021 attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc ggcctttatt 
     1081 cacattcttg cccgcctgat gaatgctcat ccggaattcc gtatggcaat gaaagacggt 
     1141 gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga gcaaactgaa 
     1201 acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct acacatatat 
     1261 tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg gtttattgag 
     1321 aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga tttaaacgtg 
     1381 gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc 
     1441 gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga tggcttccat 
     1501 gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggg cggggcgtaa 
     1561 acgcgtggat ccggcttact aaaagccaga taacagtatg cgtatttgcg cgctgatttt 
     1621 tgcggtataa gaatatatac tgatatgtat acccgaagta tgtcaaaaag aggtatgcta 
     1681 tgaagcagcg tattacagtg acagttgaca gcgacagcta tcagttgctc aaggcatata 
     1741 tgatgtcaat atctccggtc tggtaagcac aaccatgcag aatgaagccc gtcgtctgcg 
     1801 tgccgaacgc tggaaagcgg aaaatcagga agggatggct gaggtcgccc ggtttattga 
     1861 aatgaacggc tcttttgctg acgagaacag gggctggtga aatgcagttt aaggtttaca 
     1921 cctataaaag agagagccgt tatcgtctgt ttgtggatgt acagagtgat attattgaca 
     1981 cgcccgggcg acggatggtg atccccctgg ccagtgcacg tctgctgtca gataaagtct 
     2041 cccgtgaact ttacccggtg gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg 
     2101 atatggccag tgtgccggtc tccgttatcg gggaagaagt ggctgatctc agccaccgcg 
     2161 aaaatgacat caaaaacgcc attaacctga tgttctgggg aatataaatg tcaggctccc 
     2221 ttatacacag ccagtctgca ggtcgaccat agtgactgga tatgttgtgt tttacagtat 
     2281 tatgtagtct gttttttatg caaaatctaa tttaatatat tgatatttat atcattttac 
     2341 gtttctcgtt cagctttctt gtacaaagtg gtgatgggac gtccgcggag atctacgcgt 
     2401 gtcgactcga gatatccaac tagtttataa gcggccatgc tagagtccgc aaaaatcacc 
     2461 agtctctctc tacaaatcta tctctctcta tttttctcca gaataatgtg tgagtagttc 
     2521 ccagataagg gaattagggt tcttataggg tttcgctcat gtgttgagca tataagaaac 
     2581 ccttagtatg tatttgtatt tgtaaaatac ttctatcaat aaaatttcta attcctaaaa 
     2641 ccaaaatcca gtgacctgca ggcatgcgac gtcgggccct ctagaggatc cccgggtacc 
     2701 gcgaattatc gatcatgagc ggagaattaa gggagtcacg ttatgacccc cgccgatgac 
     2761 gcgggacaag ccgttttacg tttggaactg acagaaccgc aacgttgaag gagccactga 
     2821 gccgcgggtt tctggagttt aatgagctaa gcacatacgt cagaaaccat tattgcgcgt 
     2881 tcaaaagtcg cctaaggtca ctatcagcta gcaaatattt cttgtcaaaa atgctccact 
     2941 gacgttccat aaattcccct cggtatccaa ttagagtctc atattcactc tcaactcgat 
     3001 cgaggggatc taccatgagc ccagaacgac gcccggccga catccgccgt gccaccgagg 
     3061 cggacatgcc ggcggtctgc accatcgtca accactacat cgagacaagc acggtcaact 
     3121 tccgtaccga gccgcaggaa ccgcaggagt ggacggacga cctcgtccgt ctgcgggagc 
     3181 gctatccctg gctcgtcgcc gaggtggacg gcgaggtcgc cggcatcgcc tacgcgggtc 
     3241 cctggaaggc acgcaacgcc tacgactgga cggccgagtc gaccgtgtac gtctcccccc 
     3301 gccaccagcg gacgggactg ggctccacgc tctacaccca cctgctgaag tccctggagg 
     3361 cacagggctt caagagcgtg gtcgctgtca tcgggctgcc caacgacccg agcgtgcgca 
     3421 tgcacgaggc gctcggatat gccccccgcg gcatgctgcg ggcggccggc ttcaagcacg 
     3481 ggaactggca tgacgtgggt ttctggcagc tggacttcag cctgccggtg ccgccccgtc 
     3541 cggtcctgcc cgtcaccgaa atctgatgac ccctagagtc aagcagatcg ttcaaacatt 
     3601 tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa 
     3661 tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg 
     3721 agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa 
     3781 atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt actagatcga 
     3841 ccggcatgca agctgataat tcaattcggc gttaattcag tacattaaaa acgtccgcaa 
     3901 tgtgttatta agttgtctaa gcgtcaattt gtttacacca caatatatcc tgccaccagc 
     3961 cagccaacag ctccccgacc ggcagctcgg cacaaaatca ccactcgata caggcagccc 
     4021 atcagtccgg gacggcgtca gcgggagagc cgttgtaagg cggcagactt tgctcatgtt 
     4081 accgatgcta ttcggaagaa cggcaactaa gctgccgggt ttgaaacacg gatgatctcg 
     4141 cggagggtag catgttgatt gtaacgatga cagagcgttg ctgcctgtga tcaattcggg 
     4201 cacgaaccca gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc 
     4261 gctcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg 
     4321 ttttcatggc ttgttatgac tgtttttttg gggtacagtc tatgcctcgg gcatccaagc 
     4381 agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc 
     4441 agcagggcag tcgccctaaa acaaagttaa acatcatggg ggaagcggtg atcgccgaag 
     4501 tatcgactca actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc 
     4561 tggccgtaca tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg 
     4621 atttgctggt tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg 
     4681 accttttgga aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca 
     4741 ccattgttgt gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat 
     4801 ttggagaatg gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca 
     4861 ttgatctggc tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag 
     4921 cggcggagga actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa 
     4981 ccttaacgct atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta 
     5041 cgttgtcccg catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg 
     5101 ccgactgggc aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac 
     5161 aggcttatct tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat 
     5221 ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg caaataatgt ctagctagaa 
     5281 attcgttcaa gccgacgccg cttcgcggcg cggcttaact caagtcgtta gatgcactaa 
     5341 gcacataatt gctcacagcc aaactatcag gtcaagtctg cttttattat ttttaagcgt 
     5401 gcataataag ccctacacaa attgggagat atatcatgca tgaccaaaat cccttaacgt 
     5461 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat 
     5521 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg 
     5581 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga 
     5641 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac 
     5701 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt 
     5761 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag 
     5821 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc 
     5881 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag 
     5941 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca 
     6001 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt 
     6061 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc 
     6121 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc 
     6181 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc 
     6241 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat 
     6301 tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc 
     6361 tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca 
     6421 tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc 
     6481 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt 
     6541 caccgtcatc accgaaacgc gcgaggcagg gtgccttgat gtgggcgccg gcggtcgagt 
     6601 ggcgacggcg cggcttgtcc gcgccctggt agattgcctg gccctaggcc agccattttt 
     6661 gagcggccag cggccgcgat aggccgacgc gaagcggcgg ggcgtaggga gcgcagcgac 
     6721 cgaagggtag gcgctttttg cagctcttcg gctgtgcgct ggccagacag ttatgcacag 
     6781 gccaggcggg ttttaagagt tttaataagt tttaaagagt tttaggcgga aaaatcgcct 
     6841 tttttctctt ttatatcagt cacttacatg tgtgaccggt tcccaatgta cggctttggg 
     6901 ttcccaatgt acgggttccg gttcccaatg tacggctttg ggttcccaat gtacgtgcta 
     6961 tccacaggaa agagaccttt tcgacctttt tcccctgcta gggcaatttg ccctagcatc 
     7021 tgctccgtac attaggaacc ggcggatgct tcgccctcga tcaggttgcg gtagcgcatg 
     7081 actaggatcg ggccagcctg ccccgcctcc tccttcaaat cgtactccgg caggtcattt 
     7141 gacccgatca gcttgcgcac ggtgaaacag aacttcttga actctccggc gctgccactg 
     7201 cgttcgtaga tcgtcttgaa caaccatctg gcttctgcct tgcctgcggc gcggcgtgcc 
     7261 aggcggtaga gaaaacggcc gatgccggga tcgatcaaaa agtaatcggg gtgaaccgtc 
     7321 agcacgtccg ggttcttgcc ttctgtgatc tcgcggtaca tccaatcagc tagctcgatc 
     7381 tcgatgtact ccggccgccc ggtttcgctc tttacgatct tgtagcggct aatcaaggct 
     7441 tcaccctcgg ataccgtcac caggcggccg ttcttggcct tcttcgtacg ctgcatggca 
     7501 acgtgcgtgg tgtttaaccg aatgcaggtt tctaccaggt cgtctttctg ctttccgcca 
     7561 tcggctcgcc ggcagaactt gagtacgtcc gcaacgtgtg gacggaacac gcggccgggc 
     7621 ttgtctccct tcccttcccg gtatcggttc atggattcgg ttagatggga aaccgccatc 
     7681 agtaccaggt cgtaatccca cacactggcc atgccggccg gccctgcgga aacctctacg 
     7741 tgcccgtctg gaagctcgta gcggatcacc tcgccagctc gtcggtcacg cttcgacaga 
     7801 cggaaaacgg ccacgtccat gatgctgcga ctatcgcggg tgcccacgtc atagagcatc 
     7861 ggaacgaaaa aatctggttg ctcgtcgccc ttgggcggct tcctaatcga cggcgcaccg 
     7921 gctgccggcg gttgccggga ttctttgcgg attcgatcag cggccgcttg ccacgattca 
     7981 ccggggcgtg cttctgcctc gatgcgttgc cgctgggcgg cctgcgcggc cttcaacttc 
     8041 tccaccaggt catcacccag cgccgcgccg atttgtaccg ggccggatgg tttgcgaccg 
     8101 tcacgccgat tcctcgggct tgggggttcc agtgccattg cagggccggc agacaaccca 
     8161 gccgcttacg cctggccaac cgcccgttcc tccacacatg gggcattcca cggcgtcggt 
     8221 gcctggttgt tcttgatttt ccatgccgcc tcctttagcc gctaaaattc atctactcat 
     8281 ttattcattt gctcatttac tctggtagct gcgcgatgta ttcagatagc agctcggtaa 
     8341 tggtcttgcc ttggcgtacc gcgtacatct tcagcttggt gtgatcctcc gccggcaact 
     8401 gaaagttgac ccgcttcatg gctggcgtgt ctgccaggct ggccaacgtt gcagccttgc 
     8461 tgctgcgtgc gctcggacgg ccggcactta gcgtgtttgt gcttttgctc attttctctt 
     8521 tacctcatta actcaaatga gttttgattt aatttcagcg gccagcgcct ggacctcgcg 
     8581 ggcagcgtcg ccctcgggtt ctgattcaag aacggttgtg ccggcggcgg cagtgcctgg 
     8641 gtagctcacg cgctgcgtga tacgggactc aagaatgggc agctcgtacc cggccagcgc 
     8701 ctcggcaacc tcaccgccga tgcgcgtgcc tttgatcgcc cgcgacacga caaaggccgc 
     8761 ttgtagcctt ccatccgtga cctcaatgcg ctgcttaacc agctccacca ggtcggcggt 
     8821 ggcccatatg tcgtaagggc ttggctgcac cggaatcagc acgaagtcgg ctgccttgat 
     8881 cgcggacaca gccaagtccg ccgcctgggg cgctccgtcg atcactacga agtcgcgccg 
     8941 gccgatggcc ttcacgtcgc ggtcaatcgt cgggcggtcg atgccgacaa cggttagcgg 
     9001 ttgatcttcc cgcacggccg cccaatcgcg ggcactgccc tggggatcgg aatcgactaa 
     9061 cagaacatcg gccccggcga gttgcagggc gcgggctaga tgggttgcga tggtcgtctt 
     9121 gcctgacccg cctttctggt taagtacagc gataaccttc atgcgttccc cttgcgtatt 
     9181 tgtttattta ctcatcgcat catatacgca gcgaccgcat gacgcaagct gttttactca 
     9241 aatacacatc acctttttag acggcggcgc tcggtttctt cagcggccaa gctggccggc 
     9301 caggccgcca gcttggcatc agacaaaccg gccaggattt catgcagccg cacggttgag 
     9361 acgtgcgcgg gcggctcgaa cacgtacccg gccgcgatca tctccgcctc gatctcttcg 
     9421 gtaatgaaaa acggttcgtc ctggccgtcc tggtgcggtt tcatgcttgt tcctcttggc 
     9481 gttcattctc ggcggccgcc agggcgtcgg cctcggtcaa tgcgtcctca cggaaggcac 
     9541 cgcgccgcct ggcctcggtg ggcgtcactt cctcgctgcg ctcaagtgcg cggtacaggg 
     9601 tcgagcgatg cacgccaagc agtgcagccg cctctttcac ggtgcggcct tcctggtcga 
     9661 tcagctcgcg ggcgtgcgcg atctgtgccg gggtgagggt agggcggggg ccaaacttca 
     9721 cgcctcgggc cttggcggcc tcgcgcccgc tccgggtgcg gtcgatgatt agggaacgct 
     9781 cgaactcggc aatgccggcg aacacggtca acaccatgcg gccggccggc gtggtggtgt 
     9841 cggcccacgg ctctgccagg ctacgcaggc ccgcgccggc ctcctggatg cgctcggcaa 
     9901 tgtccagtag gtcgcgggtg ctgcgggcca ggcggtctag cctggtcact gtcacaacgt 
     9961 cgccagggcg taggtggtca agcatcctgg ccagctccgg gcggtcgcgc ctggtgccgg 
    10021 tgatcttctc ggaaaacagc ttggtgcagc cggccgcgtg cagttcggcc cgttggttgg 
    10081 tcaagtcctg gtcgtcggtg ctgacgcggg catagcccag caggccagcg gcggcgctct 
    10141 tgttcatggc gtaatgtctc cggttctagt cgcaagtatt ctactttatg cgactaaaac 
    10201 acgcgacaag aaaacgccag gaaaagggca gggcggcagc ctgtcgcgta acttaggact 
    10261 tgtgcgacat gtcgttttca gaagacggct gcactgaacg tcagaagccg actgcactat 
    10321 agcagcggag gggttggatc aaagtacttt aaagtacttt aaagtacttt aaagtacttt 
    10381 gatcccgagg ggaaccctgt ggttggcatg cacatacaaa tggacgaacg gataaacctt 
    10441 ttcacgccct tttaaatatc cgttattcta ataaacgctc ttttctctta ggtttacccg 
    10501 ccaatatatc ctgtcaaaca ctgatagttt aaactgaagg cgggaaacga caatctgatc 
    10561 caagctcaag ctgctctagc caatacgcaa accgcctctc cccgcgcgtt ggccgattca 
    10621 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat 
    10681 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg 
    10741 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga 
    10801 ttacgaattc gagctcgg 
//
