LOCUS       pNX35-DEST         8559 bp    DNA     circular     13-JUL-2011
DEFINITION  mbSUS.
KEYWORDS    Split-Ubiquitin.
SOURCE      
  ORGANISM  
REFERENCE   
  AUTHORS   Christopher Grefen
FEATURES             Location/Qualifiers
     rep_origin      3110..4279
                     /label=pUC\ORI
     CDS             7157..7831
                     /label=TRP1
     rep_origin      7882..8546
                     /label=f1\ORI
     CDS             1299..1958
                     /label=CmR
     CDS             2300..2605
                     /label=ccdB
     misc_recomb     complement(2646..2770)
                     /label=attR2
     CDS             2784..2885
                     /label=3HA
     CDS             complement(4281..5141)
                     /label=AmpR
     rep_origin      5272..6619
                     /label=2µ\ORI
     promoter        357..713
                     /label=ADH
     CDS             719..829
                     /label=NubG
     CDS             839..895
                     /label=2xHA
     misc_recomb     902..1026
                     /label=attR1
BASE COUNT     2363 a      1847 c      1971 g      2378 t 
ORIGIN
        1 gagctcgata tccttttgtt gtttccgggt gtacaatatg gacttcctct tttctggcaa 
       61 ccaaacccat acatcgggat tcctataata ccttcgttgg tctccctaac atgtaggtgg 
      121 cggaggggag atatacaata gaacagatac cagacaagac ataatgggct aaacaagact 
      181 acaccaatta cactgcctca ttgatggtgg tacataacga actaatactg tagccctaga 
      241 cttgatagcc atcatcatat cgaagtttca ctaccctttt tccatttgcc atctattgaa 
      301 gtaataatag gcgcatgcaa cttcttttct ttttttttct tttctctctc ccccgttgtt 
      361 gtctcaccat atccgcaatg acaaaaaaat gatggaagac actaaaggaa aaaattaacg 
      421 acaaagacag caccaacaga tgtcgttgtt ccagagctga tgaggggtat ctcgaagcac 
      481 acgaaacttt ttccttcctt cattcacgca cactactctc taatgagcaa cggtatacgg 
      541 ccttccttcc agttacttga atttgaaata aaaaaaagtt tgctgtcttg ctatcaagta 
      601 taaatagacc tgcaattatt aatcttttgt ttcctcgtca ttgttctcgt tccctttctt 
      661 ccttgtttct ttttctgcac aatatttcaa gctataccaa gcatacaatc aactcgagat 
      721 gcagattttc gtcaagactt tgaccggtaa aaccggaaca ttggaagttg aatcttccga 
      781 taccatcgac aacgttaagt cgaaaattca agacaaggaa ggaatccctg gtgggtcgta 
      841 cccatacgat gttcctgact atgcgggcta tccctatgac gtcccggact atgcaggatc 
      901 cacaagtttg tacaaaaaag ctgaacgaga aacgtaaaat gatataaata tcaatatatt 
      961 aaattagatt ttgcataaaa aacagactac ataatactgt aaaacacaac atatccagtc 
     1021 actatggcgg ccgcgggtga tgctgccaac ttagcggccg taagttgcga tcacccgacg 
     1081 cactttgcgc cgaataaata cctgtgacgg aagatcactt cgcagaataa ataaatcctg 
     1141 gtgtccctgt tgataccggg aagccctggg ccaacttttg gcgaaaatga gacgttgatc 
     1201 ggcacgcaag aggttccaac tttcaccata atgaaataag atcactaccg ggcgtatttt 
     1261 ttgagttatc gagattttca ggagctaagg aagctaaaat ggagaaaaaa atcactggat 
     1321 ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca tttcagtcag 
     1381 ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg 
     1441 taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga 
     1501 atgctcatcc ggaattccgt atggcaatga aagacggtga gctggtgata tgggatagtg 
     1561 ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg ctctggagtg 
     1621 aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg gcgtgttacg 
     1681 gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc gtctcagcca 
     1741 atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac aacttcttcg 
     1801 cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg atgccgctgg 
     1861 cgattcaggt tcatcatgcc gtttgtgatg gcttccatgt cggcagaatg cttaatgaat 
     1921 tacaacagta ctgcgatgag tggcagggcg gggcgtaaac gcgtggatcc ggcttactaa 
     1981 aagccagata acagtatgcg tatttgcgcg ctgatttttg cggtataaga atatatactg 
     2041 atatgtatac ccgaagtatg tcaaaaagag gtatgctatg aagcagcgta ttacagtgac 
     2101 agttgacagc gacagctatc agttgctcaa ggcatatatg atgtcaatat ctccggtctg 
     2161 gtaagcacaa ccatgcagaa tgaagcccgt cgtctgcgtg ccgaacgctg gaaagcggaa 
     2221 aatcaggaag ggatggctga ggtcgcccgg tttattgaaa tgaacggctc ttttgctgac 
     2281 gagaacaggg gctggtgaaa tgcagtttaa ggtttacacc tataaaagag agagccgtta 
     2341 tcgtctgttt gtggatgtac agagtgatat tattgacacg cccgggcgac ggatggtgat 
     2401 ccccctggcc agtgcacgtc tgctgtcaga taaagtctcc cgtgaacttt acccggtggt 
     2461 gcatatcggg gatgaaagct ggcgcatgat gaccaccgat atggccagtg tgccggtctc 
     2521 cgttatcggg gaagaagtgg ctgatctcag ccaccgcgaa aatgacatca aaaacgccat 
     2581 taacctgatg ttctggggaa tataaatgtc aggctccctt atacacagcc agtctgcagg 
     2641 tcgaccatag tgactggata tgttgtgttt tacagtatta tgtagtctgt tttttatgca 
     2701 aaatctaatt taatatattg atatttatat cattttacgt ttctcgttca gctttcttgt 
     2761 acaaagtggt tggtggtggc ggatacccat acgatgttcc tgactatgcg ggctatccct 
     2821 atgacgtccc ggactatgca ggatcctatc catatgacgt tccagattac gctgctcagt 
     2881 gctgaggcgc gccacttcta aataagcgaa tttcttatga tttatgattt ttattattaa 
     2941 ataagttata aaaaaaataa gtgtatacaa attttaaagt gactcttagg ttttaaaacg 
     3001 aaaattctta ttcttgagta actctttcct gtaggtcagg ttgctttctc aggtatagta 
     3061 tgaggtcgct cttattgacc acacctctac cggcagcatg caagcttggc gtaatcatgg 
     3121 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc 
     3181 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg 
     3241 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc 
     3301 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact 
     3361 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta 
     3421 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag 
     3481 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc 
     3541 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta 
     3601 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg 
     3661 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc 
     3721 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac 
     3781 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac 
     3841 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg 
     3901 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga 
     3961 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt 
     4021 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag 
     4081 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct 
     4141 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg 
     4201 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat 
     4261 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc 
     4321 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg 
     4381 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct 
     4441 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca 
     4501 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg 
     4561 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg 
     4621 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc 
     4681 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag 
     4741 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg 
     4801 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag 
     4861 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat 
     4921 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg 
     4981 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca 
     5041 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca 
     5101 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat 
     5161 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag 
     5221 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgaacgaagc 
     5281 atctgtgctt cattttgtag aacaaaaatg caacgcgaga gcgctaattt ttcaaacaaa 
     5341 gaatctgagc tgcattttta cagaacagaa atgcaacgcg aaagcgctat tttaccaacg 
     5401 aagaatctgt gcttcatttt tgtaaaacaa aaatgcaacg cgagagcgct aatttttcaa 
     5461 acaaagaatc tgagctgcat ttttacagaa cagaaatgca acgcgagagc gctattttac 
     5521 caacaaagaa tctatacttc ttttttgttc tacaaaaatg catcccgaga gcgctatttt 
     5581 tctaacaaag catcttagat tacttttttt ctcctttgtg cgctctataa tgcagtctct 
     5641 tgataacttt ttgcactgta ggtccgttaa ggttagaaga aggctacttt ggtgtctatt 
     5701 ttctcttcca taaaaaaagc ctgactccac ttcccgcgtt tactgattac tagcgaagct 
     5761 gcgggtgcat tttttcaaga taaaggcatc cccgattata ttctataccg atgtggattg 
     5821 cgcatacttt gtgaacagaa agtgatagcg ttgatgattc ttcattggtc agaaaattat 
     5881 gaacggtttc ttctattttg tctctatata ctacgtatag gaaatgttta cattttcgta 
     5941 ttgttttcga ttcactctat gaatagttct tactacaatt tttttgtcta aagagtaata 
     6001 ctagagataa acataaaaaa tgtagaggtc gagtttagat gcaagttcaa ggagcgaaag 
     6061 gtggatgggt aggttatata gggatatagc acagagatat atagcaaaga gatacttttg 
     6121 agcaatgttt gtggaagcgg tattcgcaat attttagtag ctcgttacag tccggtgcgt 
     6181 ttttggtttt ttgaaagtgc gtcttcagag cgcttttggt tttcaaaagc gctctgaagt 
     6241 tcctatactt tctagagaat aggaacttcg gaataggaac ttcaaagcgt ttccgaaaac 
     6301 gagcgcttcc gaaaatgcaa cgcgagctgc gcacatacag ctcactgttc acgtcgcacc 
     6361 tatatctgcg tgttgcctgt atatatatat acatgagaag aacggccata gtgcgtgttt 
     6421 atgcttaaat gcgtacttat atgcgtctat ttatgtagga tgaaaggtag tctagtacct 
     6481 cctgtgatat tatcccattc catgcggggt atcgtatgct tccttcagca ctacccttta 
     6541 gctgttctat atgctgccac tcctcaattg gattagtctc atccttcaat gctatcattt 
     6601 cctttgatat tggatcatac taagaaacca ttattatcat gacattaacc tataaaaata 
     6661 ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac 
     6721 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag 
     6781 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat 
     6841 cagagcagat tgtactgaga gtgcaccata gatcaacgac attactatat atataatata 
     6901 ggaagcattt aatagaacag catcgtaata tatgtgtact ttgcagttat gacgccagat 
     6961 ggcagtagtg gaagatattc tttattgaaa aatagcttgt caccttacgt acaatcttga 
     7021 tccggagctt ttcttttttt gccgattaag aattaattcg gtcgaaaaaa gaaaaggaga 
     7081 gggccaagag ggagggcatt ggtgactatt gagcacgtga gtatacgtga ttaagcacac 
     7141 aaaggcagct tggagtatgt ctgttattaa tttcacaggt agttctggtc cattggtgaa 
     7201 agtttgcggc ttgcagagca cagaggccgc agaatgtgct ctagattccg atgctgactt 
     7261 gctgggtatt atatgtgtgc ccaatagaaa gagaacaatt gacccggtta ttgcaaggaa 
     7321 aatttcaagt cttgtaaaag catataaaaa tagttcaggc actccgaaat acttggttgg 
     7381 cgtgtttcgt aatcaaccta aggaggatgt tttggctctg gtcaatgatt acggcattga 
     7441 tatcgtccaa ctgcatggag atgagtcgtg gcaagaatac caagagttcc tcggtttgcc 
     7501 agttattaaa agactcgtat ttccaaaaga ctgcaacata ctactcagtg cagcttcaca 
     7561 gaaacctcat tcgtttattc ccttgtttga ttcagaagca ggtgggacag gtgaactttt 
     7621 ggattggaac tcgatttctg actgggttgg aaggcaagag agccccgaaa gcttacattt 
     7681 tatgttagct ggtggactga cgccagaaaa tgttggtgat gcgcttagat taaatggcgt 
     7741 tattggtgtt gatgtaagcg gaggtgtgga gacaaatggt gtaaaagact ctaacaaaat 
     7801 agcaaatttc gtcaaaaatg ctaagaaata ggttattact gagtagtatt tatttaagta 
     7861 ttgtttgtgc acttgccgat ctatgcggtg tgaaataccg cacagatgcg taaggagaaa 
     7921 ataccgcatc aggaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt 
     7981 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa 
     8041 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag 
     8101 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt 
     8161 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac 
     8221 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag 
     8281 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg 
     8341 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc cattcgccat 
     8401 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc 
     8461 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt 
     8521 cacgacgttg taaaacgacg gccagtgcca agctttcgc 
//
