LOCUS       pNubWtXgate        8118 bp    DNA     circular      3-NOV-2006
DEFINITION  mbSUS.
KEYWORDS    Split-Ubiquitin.
SOURCE      
  ORGANISM  
FEATURES             Location/Qualifiers
     CDS             complement(7128..7988)
                     /label=AmpR
     rep_origin      1..1348
                     /label=2µ\ORI
     rep_origin      5957..7128
                     /label=pUC\ORI
     CDS             1884..2558
                     /label=TRP1
     rep_origin      2609..3277
                     /label=f1\ORI
     promoter        3650..4006
                     /label=ADH
     misc_feature    4130..4166
                     /label=B1\Linker
     misc_feature    complement(5651..5689)
                     /label=B2\Linker
     CDS             4011..4129
                     /label=NubWt
     CDS             4586..5395
                     /label=KanMX
BASE COUNT     2211 a      1765 c      1848 g      2294 t 
ORIGIN
        1 gaacgaagca tctgtgcttc attttgtaga acaaaaatgc aacgcgagag cgctaatttt 
       61 tcaaacaaag aatctgagct gcatttttac agaacagaaa tgcaacgcga aagcgctatt 
      121 ttaccaacga agaatctgtg cttcattttt gtaaaacaaa aatgcaacgc gagagcgcta 
      181 atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa cgcgagagcg 
      241 ctattttacc aacaaagaat ctatacttct tttttgttct acaaaaatgc atcccgagag 
      301 cgctattttt ctaacaaagc atcttagatt actttttttc tcctttgtgc gctctataat 
      361 gcagtctctt gataactttt tgcactgtag gtccgttaag gttagaagaa ggctactttg 
      421 gtgtctattt tctcttccat aaaaaaagcc tgactccact tcccgcgttt actgattact 
      481 agcgaagctg cgggtgcatt ttttcaagat aaaggcatcc ccgattatat tctataccga 
      541 tgtggattgc gcatactttg tgaacagaaa gtgatagcgt tgatgattct tcattggtca 
      601 gaaaattatg aacggtttct tctattttgt ctctatatac tacgtatagg aaatgtttac 
      661 attttcgtat tgttttcgat tcactctatg aatagttctt actacaattt ttttgtctaa 
      721 agagtaatac tagagataaa cataaaaaat gtagaggtcg agtttagatg caagttcaag 
      781 gagcgaaagg tggatgggta ggttatatag ggatatagca cagagatata tagcaaagag 
      841 atacttttga gcaatgtttg tggaagcggt attcgcaata ttttagtagc tcgttacagt 
      901 ccggtgcgtt tttggttttt tgaaagtgcg tcttcagagc gcttttggtt ttcaaaagcg 
      961 ctctgaagtt cctatacttt ctagagaata ggaacttcgg aataggaact tcaaagcgtt 
     1021 tccgaaaacg agcgcttccg aaaatgcaac gcgagctgcg cacatacagc tcactgttca 
     1081 cgtcgcacct atatctgcgt gttgcctgta tatatatata catgagaaga acggcatagt 
     1141 gcgtgtttat gcttaaatgc gtacttatat gcgtctattt atgtaggatg aaaggtagtc 
     1201 tagtacctcc tgtgatatta tcccattcca tgcggggtat cgtatgcttc cttcagcact 
     1261 accctttagc tgttctatat gctgccactc ctcaattgga ttagtctcat ccttcaatgc 
     1321 tatcatttcc tttgatattg gatcatatta agaaaccatt attatcatga cattaaccta 
     1381 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa 
     1441 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag 
     1501 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta 
     1561 tgcggcatca gagcagattg tactgagagt gcaccataga tcaacgacat tactatatat 
     1621 ataatatagg aagcatttaa tagacagcat cgtaatatat gtgtactttg cagttatgac 
     1681 gccagatggc agtagtggaa gatattcttt attgaaaaat agcttgtcac cttacgtaca 
     1741 atcttgatcc ggagcttttc tttttttgcc gattaagaat taattcggtc gaaaaaagaa 
     1801 aaggagaggg ccaagaggga gggcattggt gactattgag cacgtgagta tacgtgatta 
     1861 agcacacaaa ggcagcttgg agtatgtctg ttattaattt cacaggtagt tctggtccat 
     1921 tggtgaaagt ttgcggcttg cagagcacag aggccgcaga atgtgctcta gattccgatg 
     1981 ctgacttgct gggtattata tgtgtgccca atagaaagag aacaattgac ccggttattg 
     2041 caaggaaaat ttcaagtctt gtaaaagcat ataaaaatag ttcaggcact ccgaaatact 
     2101 tggttggcgt gtttcgtaat caacctaagg aggatgtttt ggctctggtc aatgattacg 
     2161 gcattgatat cgtccaactg catggagatg agtcgtggca agaataccaa gagttcctcg 
     2221 gtttgccagt tattaaaaga ctcgtatttc caaaagactg caacatacta ctcagtgcag 
     2281 cttcacagaa acctcattcg tttattccct tgtttgattc agaagcaggt gggacaggtg 
     2341 aacttttgga ttggaactcg atttctgact gggttggaag gcaagagagc cccgaaagct 
     2401 tacattttat gttagctggt ggactgacgc cagaaaatgt tggtgatgcg cttagattaa 
     2461 atggcgttat tggtgttgat gtaagcggag gtgtggagac aaatggtgta aaagactcta 
     2521 acaaaatagc aaatttcgtc aaaaatgcta agaaataggt tattactgag tagtatttat 
     2581 ttaagtattg tttgtgcact tgccgatcta tgcggtgtga aataccgcac agatgcgtaa 
     2641 ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa 
     2701 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa 
     2761 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact 
     2821 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc 
     2881 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa 
     2941 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc 
     3001 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt 
     3061 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcgcg 
     3121 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct 
     3181 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg 
     3241 gttttcccag tcacgacgtt gtaaaacgac ggccagtcgt ccaagctttc gcgagctcga 
     3301 tatccttttg ttgtttccgg gtgtacaata tggacttcct cttttctggc aaccaaaccc 
     3361 atacatcggg attcctataa taccttcgtt ggtctcccta acatgtaggt ggcggagggg 
     3421 agatatacaa tagaacagat accagacaag acataatggg ctaaacaaga ctacaccaat 
     3481 tacactgcct cattgatggt ggtacataac gaactaatac tgtagcccta gacttgatag 
     3541 ccatcatcat atcgaagttt cactaccctt tttccatttg ccatctattg aagtaataat 
     3601 aggcgcatgc aacttctttt cttttttttt cttttctctc tcccccgttg ttgtctcacc 
     3661 atatccgcaa tgacaaaaaa aatgatggaa gacactaaag gaaaaaatta acgacaaaga 
     3721 cagcaccaac agatgtcgtt gttccagagc tgatgagggg tatcttcgaa cacacgaaac 
     3781 tttttccttc cttcattcac gcacactact ctctaatgag caacggtata cggccttcct 
     3841 tccagttact tgaatttgaa ataaaaaaag tttgccgctt tgctatcaag tataaataga 
     3901 cctgcaatta ttaatctttt gtttcctcgt cattgttctc gttccctttc ttccttgttt 
     3961 ctttttctgc acaatatttc aagctatacc aagcatacaa tcaactcgag atgcagattt 
     4021 tcgtcaagac tttgaccggt aaaaccataa cattggaagt tgaatcttcc gataccatcg 
     4081 acaacgttaa gtcgaaaatt caagacaagg aaggaatccc tggtgggtcg acaagtttgt 
     4141 acaaaaaagc aggctctcca accaccatga attctatacg aagttattag gtctagagat 
     4201 ctgtttagct tgcctcgtcc ccgccgggtc acccggccag cgacatggag gcccagaata 
     4261 ccctccttga cagtcttgac gtgcgcagct caggggcatg atgtgactgt cgcccgtaca 
     4321 tttagcccat acatccccat gtataatcat ttgcatccat acattttgat ggccgcacgg 
     4381 cgcgaagcaa aaattacggc tcctcgctgc agacctgcga gcagggaaac gctcccctca 
     4441 cagacgcgtt gaattgtccc cacgccgcgc ccctgtagag aaatataaaa ggttaggatt 
     4501 tgccactgag gttcttcttt catatacttc cttttaaaat cttgctagga tacagttctc 
     4561 acatcacatc cgaacataaa caaccatggg taaggaaaag actcacgttt cgaggccgcg 
     4621 attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg 
     4681 gcaatcaggt gcgacaatct atcgattgta tgggaagccc gatgcgccag agttgtttct 
     4741 gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg 
     4801 gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc 
     4861 atggttactc accactgcga tccccggcaa aacagcattc caggtattag aagaatatcc 
     4921 tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat 
     4981 tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc 
     5041 acgaatgaat aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc 
     5101 tgttgaacaa gtctggaaag aaatgcataa gcttttgcca ttctcaccgg attcagtcgt 
     5161 cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg 
     5221 tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa 
     5281 ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga 
     5341 taatcctgat atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaatcagt 
     5401 actgacaata aaaagattct tgttttcaag aacttgtcat ttgtatagtt tttttatatt 
     5461 gtagttgttc tattttaatc aaatgttagc gtgatttata ttttttttcg cctcgacatc 
     5521 atctgcccag atgcgaagtt aagtgcgcag aaagtaatat catgcgtcaa tcgtatgtga 
     5581 atgctggtcg ctatactgct gtcgattcga tactaacgcc gccatccagt gtcgaaaacg 
     5641 agctcccggg tacccagctt tcttgtacaa agtggttggt ggtggcggat ccggtggagg 
     5701 tggatcatac ccatacgacg ttccagatta tgcttaacag ccaagctaat tccgggcgaa 
     5761 tttcttatga tttatgattt ttattattaa ataagttata aaaaaaataa gtgtatacaa 
     5821 attttaaagt gactcttagg ttttaaaacg aaaattcttg ttcttgagta actctttcct 
     5881 gtaggtcagg ttgctttctc aggtatagca tgaggtcgct cttattgacc acacctctac 
     5941 cggcatgcaa gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg 
     6001 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa 
     6061 tgagtgaggt aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac 
     6121 ctgtcgtgcc agctggatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt 
     6181 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga 
     6241 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca 
     6301 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg 
     6361 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt 
     6421 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc 
     6481 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct 
     6541 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc 
     6601 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta 
     6661 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca 
     6721 gccactagta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag 
     6781 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag 
     6841 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt 
     6901 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa 
     6961 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg 
     7021 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga 
     7081 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta 
     7141 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc 
     7201 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg 
     7261 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga 
     7321 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt 
     7381 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt 
     7441 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc 
     7501 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc 
     7561 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca 
     7621 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag 
     7681 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg 
     7741 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa 
     7801 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa 
     7861 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga 
     7921 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga 
     7981 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg 
     8041 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt 
     8101 ccccgaaaag tgccacct 
//
