LOCUS       pMetYC-DEST       10186 bp    DNA     circular      1-SEP-2009
DEFINITION  mbSUS.
KEYWORDS    Split-Ubiquitin.
SOURCE      
  ORGANISM
REFERENCE   
  AUTHORS   Christopher Grefen
FEATURES             Location/Qualifiers
     promoter        1..382
                     /label=met25
     misc_feature    386..510
                     /label=attR1
     CDS             619..1278
                     /label=CmR
     CDS             1620..1925
                     /label=ccdB
     misc_feature    complement(1966..2090)
                     /label=attR2
     CDS             3303..3528
                     /label=VP16
     CDS             2125..2253
                     /label=Cub
     CDS             2656..3261
                     /label=LexA
     CDS             2269..2643
                     /label=ProteinA
     rep_origin      5195..5874
                     /label=ARS1-ORI
     CDS             complement(6397..7503)
                     /label=LEU2
     CDS             8108..8968
                     /label=AmpR
     centromere      4314..5165
                     /label=CEN4
     rep_origin      9113..9786
                     /label=pUC
BASE COUNT     2863 a      2236 c      2324 g      2763 t 
ORIGIN
        1 ggatgcaagg gttcgaatcc cttagctctc attatttttt gctttttctc ttgaggtcac 
       61 atgatcgcaa aatggcaaat ggcacgtgaa gctgtcgata ttggggaact gtggtggttg 
      121 gcaaatgact aattaagtta gtcaaggcgc catcctcatg aaaactgtgt aacataataa 
      181 ccgaagtgtc gaaaaggtgg caccttgtcc aattgaacac gctcgatgaa aaaaataaga 
      241 tatatataag gttaagtaaa gcgtctgtta gaaaggaagt ttttcctttt tcttgctctc 
      301 ttgtcttttc atctactatt tccttcgtgt aatacagggt cgtcagatac atagatacaa 
      361 ttctattacc cccatccata caatcacaag tttgtacaaa aaagctgaac gagaaacgta 
      421 aaatgatata aatatcaata tattaaatta gattttgcat aaaaaacaga ctacataata 
      481 ctgtaaaaca caacatatcc agtcatattg gcggccgcat taggcacccc aggctttaca 
      541 ctttatgctt ccggctcgta taatgtgtgg attttgagtt aggatccgtc gagattttca 
      601 ggagctaagg aagctaaaat ggagaaaaaa atcactggat ataccaccgt tgatatatcc 
      661 caatggcatc gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg tacctataac 
      721 cagaccgttc agctggatat tacggccttt ttaaagaccg taaagaaaaa taagcacaag 
      781 ttttatccgg cctttattca cattcttgcc cgcctgatga atgctcatcc ggaattccgt 
      841 atggcaatga aagacggtga gctggtgata tgggatagtg ttcacccttg ttacaccgtt 
      901 ttccatgagc aaactgaaac gttttcatcg ctctggagtg aataccacga cgatttccgg 
      961 cagtttctac acatatattc gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc 
     1021 cctaaagggt ttattgagaa tatgtttttc gtctcagcca atccctgggt gagtttcacc 
     1081 agttttgatt taaacgtggc caatatggac aacttcttcg cccccgtttt caccatgggc 
     1141 aaatattata cgcaaggcga caaggtgctg atgccgctgg cgattcaggt tcatcatgcc 
     1201 gtttgtgatg gcttccatgt cggcagaatg cttaatgaat tacaacagta ctgcgatgag 
     1261 tggcagggcg gggcgtaaac gcgtggatcc ggcttactaa aagccagata acagtatgcg 
     1321 tatttgcgcg ctgatttttg cggtataaga atatatactg atatgtatac ccgaagtatg 
     1381 tcaaaaagag gtatgctatg aagcagcgta ttacagtgac agttgacagc gacagctatc 
     1441 agttgctcaa ggcatatatg atgtcaatat ctccggtctg gtaagcacaa ccatgcagaa 
     1501 tgaagcccgt cgtctgcgtg ccgaacgctg gaaagcggaa aatcaggaag ggatggctga 
     1561 ggtcgcccgg tttattgaaa tgaacggctc ttttgctgac gagaacaggg gctggtgaaa 
     1621 tgcagtttaa ggtttacacc tataaaagag agagccgtta tcgtctgttt gtggatgtac 
     1681 agagtgatat tattgacacg cccgggcgac ggatggtgat ccccctggcc agtgcacgtc 
     1741 tgctgtcaga taaagtctcc cgtgaacttt acccggtggt gcatatcggg gatgaaagct 
     1801 ggcgcatgat gaccaccgat atggccagtg tgccggtctc cgttatcggg gaagaagtgg 
     1861 ctgatctcag ccaccgcgaa aatgacatca aaaacgccat taacctgatg ttctggggaa 
     1921 tataaatgtc aggctccctt atacacagcc agtctgcagg tcgaccatag tgactggata 
     1981 tgttgtgttt tacagcatta tgtagtctgt tttttatgca aaatctaatt taatatattg 
     2041 atatttatat cattttacgt ttctcgttca gctttcttgt acaaagtggt gattggtggt 
     2101 ggcggatccg gtggaggtgg atcaggtatc cctccagatc aacaaagatt gatctttgcc 
     2161 ggtaagcagc tagaagacgg tagaacgctg tctgattaca acattcagaa ggagtccacc 
     2221 ttacatcttg tgctaaggct aagaggtggt atgcacagat cagcttgcgg ccgcatggca 
     2281 ggccttgcgc aacacgatga agccgtggac aacaaattca acaaagaaca acaaaacgcg 
     2341 ttctatgaga tcttacattt acctaactta aacgaagaac aacgaaacgc cttcatccaa 
     2401 agtttaaaag atgacccaag ccaaagcgct aaccttttag cagaagctaa aaagctaaat 
     2461 gatgctcagg cgccgaaagt agacaacaaa ttcaacaaag aacaacaaaa cgcgttctat 
     2521 gagatcttac atttacctaa cttaaacgaa gaacaacgaa acgccttcat ccaaagttta 
     2581 aaagatgacc caagccaaag cgctaacctt ttagcagaag ctaaaaagct aaatgatgct 
     2641 caggcgccga aagtagacgc gagcggccgc atgaaagcgt taacggccag gcaacaagag 
     2701 gtgtttgatc tcatccgtga tcacatcagc cagacaggta tgccgccgac gcgtgcggaa 
     2761 atcgcgcagc gtttggggtt ccgttcccca aacgcggctg aagaacatct gaaggcgctg 
     2821 gcacgcaaag gcgttattga aattgtttcc ggcgcatcac gcgggatccg tctgttgcag 
     2881 gaagaggaag aagggttgcc gctggtaggt cgtgtggctg ccggtgaacc acttctggcg 
     2941 caacagcata ttgaaggtca ttatcaggtc gacccttcct tattcaagcc gaatgctgat 
     3001 ttcctgctgc gcgtcagcgg gatgtcgatg aaagatatcg gcattatgga tggtgacttg 
     3061 ctggcagtgc ataaaactca ggatgtacgt aacggtcagg tcgttgtcgc acgtattgat 
     3121 gacgaggtta ccgttaagcg cctgaaaaaa cagggcaata aagtcgaact gttgccagaa 
     3181 aatagcgagt ttaaaccaat tgtcgtagat cttcgtcagc agagcttcac cattgaaggg 
     3241 ctggcggttg gggttattcg caacggcgac tggctggaat tcccggggat ctgggccccc 
     3301 ccgaccgatg tcagcctggg ggacgagctc cacttagacg gcgaggacgt ggcgatggcg 
     3361 catgccgacg cgctagacga tttcgatctg gacatgttgg gggacgggga ttccccgggt 
     3421 ccgggattta ccccccacga ctccgccccc tacggcgctc tggatatggc cgacttcgag 
     3481 tttgagcaga tgtttaccga tgcccttgga attgacgagt acggtgggta gggggcgcga 
     3541 ccggacccgc atcccccgtc tgggttttcc cctcccgtcc ccggttcgta tccacaataa 
     3601 acacgagcac atacattaca aaaacctgcg gttgtcgtct gattatttgg tggatccggg 
     3661 ggagatgggg gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta 
     3721 tgacggcaat aaaaagacag aataaaacgc acgggtgttg ggtcgtttgt tcataaacgc 
     3781 ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa 
     3841 tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg 
     3901 ctcgcagcca acgtcggggc ggcaggccct gccatagcca ctggccccgt gggttaggga 
     3961 cggggtcccc catggggaat ggtttatggt tcgtgggggt tattattttg ggcgttgcgt 
     4021 ggggtcaggt ccacgaccca agctggccgc caccgcggtg gagctccaat tcgccctata 
     4081 gtgagtcgta ttacaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg 
     4141 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg 
     4201 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgcc 
     4261 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata atcgctgggc 
     4321 cattctcatg aagaatatct tgaatttatt gtcatattac tagttggtgt ggaagtccta 
     4381 atatcggtga tcaatatagt ggttgacatg ctggctagtc aacattgagc cttttgatca 
     4441 tgcaaatata ttacggtatt ttacaatcaa atatcaaact taactattga ctttataact 
     4501 tatttaggtg gtaacattct tataaaaaag aaaaaaatta ctgcaaaaca gtactagctt 
     4561 ttaacttgta tcctaggtta tctatgctgt ctcaccatag agaatattac ctatttcaga 
     4621 atgtatgtcc atgattcgcc gggtaaatac atataataca caaatctggc ttaataaagt 
     4681 ctataatata tctcataaag aagtgctaaa ttggctagtg ctatatattt ttaagaaaat 
     4741 ttcttttgac taagtccata tcgactttgt aaaagttcac attagcatac atatattaca 
     4801 cgagccagaa atagtaactt ttgcctaaat cacaaattgc aaaatttaat tgcttgcaaa 
     4861 aggtcacatg cttataatca acttttttaa aaatttaaaa tactttttta ttttttattt 
     4921 ttaaacataa atgaaataat ttatttattg tttatgatta ccgaaacata aaacctgctc 
     4981 aagaaaaaga aactgttttg tccttggaaa aaaagcacta cctaggagcg gccaaaatgc 
     5041 cgaggctttc atagcttaaa ctctttacag aaaataggca ttatagatca gttcgagttt 
     5101 tcttattctt ccttccggtt ttatcgtcac agttttacag taaataagta tcacctctta 
     5161 gagttcgatg ataagctgtc aaacatgaga attaattcca catgttaaaa tagtgaagga 
     5221 gcatgttcgg cacacagtgg accgaacgtg gggtaagtgc actagggtcc ggttaaacgg 
     5281 atctcgcatt gatgaggcaa cgctaattat caacatatag attgttatct atctgcatga 
     5341 acacgaaatc tttacttgac gacttgaggc tgatggtgtt tatgcaaaga aaccactgtg 
     5401 tttaatatgt gtcactgttt gatattactg tcagcgtaga agataatagt aaaagcggtt 
     5461 aataagtgta tttgagataa gtgtgataaa gtttttacag cgaaaagacg ataaatacaa 
     5521 gaaaatgatt acgaggatac ggagagaggt atgtacatgt gtatttatat actaagctgc 
     5581 cggcggttgt ttgcaagacc gagaaaaggc tagcaagaat cgggtcattg tagcgtatgc 
     5641 gcctgtgaac attctcttca acaagtttga ttccattgcg gtgaaatggt aaaagtcaac 
     5701 cccctgcgat gtatattttc ctgtacaatc aatcaaaaag ccaaatgatt tagcattatc 
     5761 tttacatctt gttattttac agattttatg tttagatctt ttatgcttgc ttttcaaaag 
     5821 gcttgcaggc aagtgcacaa acaatactta aataaatact actcagtaat aacctatttc 
     5881 ttagcatttt tgacgaaatt tgctattttg ttagagtctt ttacaccatt tgtctccaca 
     5941 cctccgctta catcaacacc aataacgcca tttaatctaa gcgcatcacc aacattttct 
     6001 ggcgtcagtc caccagctaa cataaaatgt aagctctcgg ggctctcttg ccttccaacc 
     6061 cagtcagaaa tcgagttcca atccaaaagt tcacctgtcc cacctgcttc tgaatcaaac 
     6121 aagggaataa acgaatgagg tttctgtgaa gctgcactga gtagtatgtt gcagtctttt 
     6181 ggaaatacga gtcttttaat aactggcaaa ccgaggaact cttggtattc ttgccacgac 
     6241 tcatctccat gcagttggac gatcgatgat aagctgtcaa acatgagaat taattctacc 
     6301 ctatgaacat attccatttt gtaatttcgt gtcgtttcta ttatgaattt catttataaa 
     6361 gtttatgtac aaatatcata aaaaaagaga atctttttaa gcaaggattt tcttaacttc 
     6421 ttcggcgaca gcatcaccga cttcggtggt actgttggaa ccacctaaat caccagttct 
     6481 gatacctgca tccaaaacct ttttaactgc atcttcaatg gccttacctt cttcaggcaa 
     6541 gttcaatgac aatttcaaca tcattgcagc agacaagata gtggcgatag ggttgacctt 
     6601 attctttggc aaatctggag cagaaccgtg gcatggttcg tacaaaccaa atgcggtgtt 
     6661 cttgtctggc aaagaggcca aggacgcaga tggcaacaaa cccaaggaac ctgggataac 
     6721 ggaggcttca tcggagatga tatcaccaaa catgttgctg gtgattataa taccatttag 
     6781 gtgggttggg ttcttaacta ggatcatggc ggcagaatca atcaattgat gttgaacctt 
     6841 caatgtagga aattcgttct tgatggtttc ctccacagtt tttctccata atcttgaaga 
     6901 ggccaaaaca ttagctttat ccaaggacca aataggcaat ggtggctcat gttgtagggc 
     6961 catgaaagcg gccattcttg tgattctttg cacttctgga acggtgtatt gttcactatc 
     7021 ccaagcgaca ccatcaccat cgtcttcctt tctcttacca aagtaaatac ctcccactaa 
     7081 ttctctgaca acaacgaagt cagtaccttt agcaaattgt ggcttgattg gagataagtc 
     7141 taaaagagag tcggatgcaa agttacatgg tcttaagttg gcgtacaatt gaagttcttt 
     7201 acggattttt agtaaacctt gttcaggtct aacactacct gtaccccatt taggaccacc 
     7261 cacagcacct aacaaaacgg catcagcctt cttggaggct tccagcgcct catctggaag 
     7321 tggaacacct gtagcatcga tagcagcacc accaattaaa tgattttcga aatcgaactt 
     7381 gacattggaa cgaacatcag aaatagcttt aagaacctta atggcttcgg ctgtgatttc 
     7441 ttgaccaacg tggtcacctg gcaaaacgac gatcttctta ggggcagaca ttagaatggt 
     7501 atatccttga aatatatata tatattgctg aaatgtaaaa ggtaagaaaa gttagaaagt 
     7561 aagacgattg ctaaccacct attggaaaaa acaataggtc cttaaataat attgtcaact 
     7621 tcaagtattg tgatgcaagc atttagtcat gaacgcttct ctattctata tgaaaagccg 
     7681 gttccggcgc tctcaccttt cctttttctc ccaatttttc agttgaaaaa ggtatatgcg 
     7741 tcaggcgacc tctgaaatta acaaaaaatt tccagtcatc gaatttgatt ctgtgcgata 
     7801 gcgcccctgt gtgttctcgt tatgttgagg aaaaaaataa tggttgctaa gagattcgaa 
     7861 ctcttgcatc ttacgatacc tgagtattcc cacagttaat tcttgaagac gaaagggcct 
     7921 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg 
     7981 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc 
     8041 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag 
     8101 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg 
     8161 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt 
     8221 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt 
     8281 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt 
     8341 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa 
     8401 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag 
     8461 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac 
     8521 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac 
     8581 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac 
     8641 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac 
     8701 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact 
     8761 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg 
     8821 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt 
     8881 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat 
     8941 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta 
     9001 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa 
     9061 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga 
     9121 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac 
     9181 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt 
     9241 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc 
     9301 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat 
     9361 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag 
     9421 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc 
     9481 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag 
     9541 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac 
     9601 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg 
     9661 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct 
     9721 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc 
     9781 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga 
     9841 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga 
     9901 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg 
     9961 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt 
    10021 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt 
    10081 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc 
    10141 caagctcgaa attaaccctc actaaaggga acaaaagctg ggtacc 
//
