LOCUS       pMetOYC-Dest       10309 bp    DNA     circular      6-DEC-2012
DEFINITION  mbSUS.
KEYWORDS    Split-Ubiquitin.
SOURCE      
  ORGANISM
REFERENCE   
  AUTHORS   Christopher Grefen
FEATURES             Location/Qualifiers
     promoter        1..382
                     /vntifkey="29"
                     /label=met25
     CDS             1743..2048
                     /vntifkey="4"
                     /label=ccdB
     misc_feature    complement(2089..2213)
                     /vntifkey="21"
                     /label=attR2
     CDS             3426..3651
                     /vntifkey="4"
                     /label=VP16
     CDS             2248..2376
                     /vntifkey="4"
                     /label=Cub
     CDS             2779..3384
                     /vntifkey="4"
                     /label=LexA
     CDS             2392..2766
                     /vntifkey="4"
                     /label=ProteinA
     rep_origin      5318..5997
                     /vntifkey="33"
                     /label=ARS1-ORI
     CDS             complement(6520..7626)
                     /vntifkey="4"
                     /label=LEU2
     CDS             8231..9091
                     /vntifkey="4"
                     /label=AmpR
     centromere      4437..5288
                     /vntifkey="5"
                     /label=CEN4
     rep_origin      9236..9909
                     /vntifkey="33"
                     /label=pUC
     CDS             742..1401
                     /vntifkey="4"
                     /label=CmR
     CDS             389..496
                     /vntifkey="4"
                     /label=Ost4
     misc_recomb     509..633
                     /vntifkey="86"
                     /label=attR1
BASE COUNT     2892 a      2265 c      2350 g      2802 t 
ORIGIN
        1 ggatgcaagg gttcgaatcc cttagctctc attatttttt gctttttctc ttgaggtcac 
       61 atgatcgcaa aatggcaaat ggcacgtgaa gctgtcgata ttggggaact gtggtggttg 
      121 gcaaatgact aattaagtta gtcaaggcgc catcctcatg aaaactgtgt aacataataa 
      181 ccgaagtgtc gaaaaggtgg caccttgtcc aattgaacac gctcgatgaa aaaaataaga 
      241 tatatataag gttaagtaaa gcgtctgtta gaaaggaagt ttttcctttt tcttgctctc 
      301 ttgtcttttc atctactatt tccttcgtgt aatacagggt cgtcagatac atagatacaa 
      361 ttctattacc cccatccata cactcgagat gatctctgat gaacagttga actccttggc 
      421 catcaccttc ggtattgtga tgatgacttt aattgtcatt taccatgctg ttgactccac 
      481 catgtctcct aagaacggtg gatctagaac aagtttgtac aaaaaagctg aacgagaaac 
      541 gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac agactacata 
      601 atactgtaaa acacaacata tccagtcact atggcggccg cattaggcac cccaggcttt 
      661 acactttatg cttccggctc gtataatgtg tggattttga gttaggatcc gtcgagattt 
      721 tcaggagcta aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata 
      781 tcccaatggc atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat 
      841 aaccagaccg ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac 
      901 aagttttatc cggcctttat tcacattctt gcccgcctga tgaatgctca tccggaattc 
      961 cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc 
     1021 gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc 
     1081 cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat 
     1141 ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc 
     1201 accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg 
     1261 ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat 
     1321 gccgtttgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat 
     1381 gagtgggcag gcggggcgta aacgcgtgga tccggcttac taaaagccag ataacagtat 
     1441 gcgtatttgc gcgctgattt ttgcggtata agaatatata ctgatatgta tacccgaagt 
     1501 atgtcaaaaa gaggtatgct atgaagcagc gtattacagt gacagttgac agcgacagct 
     1561 atcagttgct caaggcatat atgatgtcaa tatctccggt ctggtaagca caaccatgca 
     1621 gaatgaagcc cgtcgtctgc gtgccgaacg ctggaaagcg gaaaatcagg aagggatggc 
     1681 tgaggtcgcc cggtttattg aaatgaacgg ctcttttgct gacgagaaca ggggctggtg 
     1741 aaatgcagtt taaggtttac acctataaaa gagagagccg ttatcgtctg tttgtggatg 
     1801 tacagagtga tattattgac acgcccgggc gacggatggt gatccccctg gccagtgcac 
     1861 gtctgctgtc agataaagtc tcccgtgaac tttacccggt ggtgcatatc ggggatgaaa 
     1921 gctggcgcat gatgaccacc gatatggcca gtgtgccggt ctccgttatc ggggaagaag 
     1981 tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg atgttctggg 
     2041 gaatataaat gtcaggctcc cttatacaca gccagtctgc aggtcgacca tagtgactgg 
     2101 atatgttgtg ttttacagca ttatgtagtc tgttttttat gcaaaatcta atttaatata 
     2161 ttgatattta tatcatttta cgtttctcgt tcagctttct tgtacaaagt ggtgattggt 
     2221 ggtggcggat ccggtggagg tggatcaggt atccctccag atcaacaaag attgatcttt 
     2281 gccggtaagc agctagaaga cggtagaacg ctgtctgatt acaacattca gaaggagtcc 
     2341 accttacatc ttgtgctaag gctaagaggt ggtatgcaca gatcagcttg cggccgcatg 
     2401 gcaggccttg cgcaacacga tgaagccgtg gacaacaaat tcaacaaaga acaacaaaac 
     2461 gcgttctatg agatcttaca tttacctaac ttaaacgaag aacaacgaaa cgccttcatc 
     2521 caaagtttaa aagatgaccc aagccaaagc gctaaccttt tagcagaagc taaaaagcta 
     2581 aatgatgctc aggcgccgaa agtagacaac aaattcaaca aagaacaaca aaacgcgttc 
     2641 tatgagatct tacatttacc taacttaaac gaagaacaac gaaacgcctt catccaaagt 
     2701 ttaaaagatg acccaagcca aagcgctaac cttttagcag aagctaaaaa gctaaatgat 
     2761 gctcaggcgc cgaaagtaga cgcgagcggc cgcatgaaag cgttaacggc caggcaacaa 
     2821 gaggtgtttg atctcatccg tgatcacatc agccagacag gtatgccgcc gacgcgtgcg 
     2881 gaaatcgcgc agcgtttggg gttccgttcc ccaaacgcgg ctgaagaaca tctgaaggcg 
     2941 ctggcacgca aaggcgttat tgaaattgtt tccggcgcat cacgcgggat ccgtctgttg 
     3001 caggaagagg aagaagggtt gccgctggta ggtcgtgtgg ctgccggtga accacttctg 
     3061 gcgcaacagc atattgaagg tcattatcag gtcgaccctt ccttattcaa gccgaatgct 
     3121 gatttcctgc tgcgcgtcag cgggatgtcg atgaaagata tcggcattat ggatggtgac 
     3181 ttgctggcag tgcataaaac tcaggatgta cgtaacggtc aggtcgttgt cgcacgtatt 
     3241 gatgacgagg ttaccgttaa gcgcctgaaa aaacagggca ataaagtcga actgttgcca 
     3301 gaaaatagcg agtttaaacc aattgtcgta gatcttcgtc agcagagctt caccattgaa 
     3361 gggctggcgg ttggggttat tcgcaacggc gactggctgg aattcccggg gatctgggcc 
     3421 cccccgaccg atgtcagcct gggggacgag ctccacttag acggcgagga cgtggcgatg 
     3481 gcgcatgccg acgcgctaga cgatttcgat ctggacatgt tgggggacgg ggattccccg 
     3541 ggtccgggat ttacccccca cgactccgcc ccctacggcg ctctggatat ggccgacttc 
     3601 gagtttgagc agatgtttac cgatgccctt ggaattgacg agtacggtgg gtagggggcg 
     3661 cgaccggacc cgcatccccc gtctgggttt tcccctcccg tccccggttc gtatccacaa 
     3721 taaacacgag cacatacatt acaaaaacct gcggttgtcg tctgattatt tggtggatcc 
     3781 gggggagatg ggggaggcta actgaaacac ggaaggagac aataccggaa ggaacccgcg 
     3841 ctatgacggc aataaaaaga cagaataaaa cgcacgggtg ttgggtcgtt tgttcataaa 
     3901 cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc 
     3961 caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca 
     4021 gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag ccactggccc cgtgggttag 
     4081 ggacggggtc ccccatgggg aatggtttat ggttcgtggg ggttattatt ttgggcgttg 
     4141 cgtggggtca ggtccacgac ccaagctggc cgccaccgcg gtggagctcc aattcgccct 
     4201 atagtgagtc gtattacaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc 
     4261 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata 
     4321 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc 
     4381 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc ataatcgctg 
     4441 ggccattctc atgaagaata tcttgaattt attgtcatat tactagttgg tgtggaagtc 
     4501 ctaatatcgg tgatcaatat agtggttgac atgctggcta gtcaacattg agccttttga 
     4561 tcatgcaaat atattacggt attttacaat caaatatcaa acttaactat tgactttata 
     4621 acttatttag gtggtaacat tcttataaaa aagaaaaaaa ttactgcaaa acagtactag 
     4681 cttttaactt gtatcctagg ttatctatgc tgtctcacca tagagaatat tacctatttc 
     4741 agaatgtatg tccatgattc gccgggtaaa tacatataat acacaaatct ggcttaataa 
     4801 agtctataat atatctcata aagaagtgct aaattggcta gtgctatata tttttaagaa 
     4861 aatttctttt gactaagtcc atatcgactt tgtaaaagtt cacattagca tacatatatt 
     4921 acacgagcca gaaatagtaa cttttgccta aatcacaaat tgcaaaattt aattgcttgc 
     4981 aaaaggtcac atgcttataa tcaacttttt taaaaattta aaatactttt ttatttttta 
     5041 tttttaaaca taaatgaaat aatttattta ttgtttatga ttaccgaaac ataaaacctg 
     5101 ctcaagaaaa agaaactgtt ttgtccttgg aaaaaaagca ctacctagga gcggccaaaa 
     5161 tgccgaggct ttcatagctt aaactcttta cagaaaatag gcattataga tcagttcgag 
     5221 ttttcttatt cttccttccg gttttatcgt cacagtttta cagtaaataa gtatcacctc 
     5281 ttagagttcg atgataagct gtcaaacatg agaattaatt ccacatgtta aaatagtgaa 
     5341 ggagcatgtt cggcacacag tggaccgaac gtggggtaag tgcactaggg tccggttaaa 
     5401 cggatctcgc attgatgagg caacgctaat tatcaacata tagattgtta tctatctgca 
     5461 tgaacacgaa atctttactt gacgacttga ggctgatggt gtttatgcaa agaaaccact 
     5521 gtgtttaata tgtgtcactg tttgatatta ctgtcagcgt agaagataat agtaaaagcg 
     5581 gttaataagt gtatttgaga taagtgtgat aaagttttta cagcgaaaag acgataaata 
     5641 caagaaaatg attacgagga tacggagaga ggtatgtaca tgtgtattta tatactaagc 
     5701 tgccggcggt tgtttgcaag accgagaaaa ggctagcaag aatcgggtca ttgtagcgta 
     5761 tgcgcctgtg aacattctct tcaacaagtt tgattccatt gcggtgaaat ggtaaaagtc 
     5821 aaccccctgc gatgtatatt ttcctgtaca atcaatcaaa aagccaaatg atttagcatt 
     5881 atctttacat cttgttattt tacagatttt atgtttagat cttttatgct tgcttttcaa 
     5941 aaggcttgca ggcaagtgca caaacaatac ttaaataaat actactcagt aataacctat 
     6001 ttcttagcat ttttgacgaa atttgctatt ttgttagagt cttttacacc atttgtctcc 
     6061 acacctccgc ttacatcaac accaataacg ccatttaatc taagcgcatc accaacattt 
     6121 tctggcgtca gtccaccagc taacataaaa tgtaagctct cggggctctc ttgccttcca 
     6181 acccagtcag aaatcgagtt ccaatccaaa agttcacctg tcccacctgc ttctgaatca 
     6241 aacaagggaa taaacgaatg aggtttctgt gaagctgcac tgagtagtat gttgcagtct 
     6301 tttggaaata cgagtctttt aataactggc aaaccgagga actcttggta ttcttgccac 
     6361 gactcatctc catgcagttg gacgatcgat gataagctgt caaacatgag aattaattct 
     6421 accctatgaa catattccat tttgtaattt cgtgtcgttt ctattatgaa tttcatttat 
     6481 aaagtttatg tacaaatatc ataaaaaaag agaatctttt taagcaagga ttttcttaac 
     6541 ttcttcggcg acagcatcac cgacttcggt ggtactgttg gaaccaccta aatcaccagt 
     6601 tctgatacct gcatccaaaa cctttttaac tgcatcttca atggccttac cttcttcagg 
     6661 caagttcaat gacaatttca acatcattgc agcagacaag atagtggcga tagggttgac 
     6721 cttattcttt ggcaaatctg gagcagaacc gtggcatggt tcgtacaaac caaatgcggt 
     6781 gttcttgtct ggcaaagagg ccaaggacgc agatggcaac aaacccaagg aacctgggat 
     6841 aacggaggct tcatcggaga tgatatcacc aaacatgttg ctggtgatta taataccatt 
     6901 taggtgggtt gggttcttaa ctaggatcat ggcggcagaa tcaatcaatt gatgttgaac 
     6961 cttcaatgta ggaaattcgt tcttgatggt ttcctccaca gtttttctcc ataatcttga 
     7021 agaggccaaa acattagctt tatccaagga ccaaataggc aatggtggct catgttgtag 
     7081 ggccatgaaa gcggccattc ttgtgattct ttgcacttct ggaacggtgt attgttcact 
     7141 atcccaagcg acaccatcac catcgtcttc ctttctctta ccaaagtaaa tacctcccac 
     7201 taattctctg acaacaacga agtcagtacc tttagcaaat tgtggcttga ttggagataa 
     7261 gtctaaaaga gagtcggatg caaagttaca tggtcttaag ttggcgtaca attgaagttc 
     7321 tttacggatt tttagtaaac cttgttcagg tctaacacta cctgtacccc atttaggacc 
     7381 acccacagca cctaacaaaa cggcatcagc cttcttggag gcttccagcg cctcatctgg 
     7441 aagtggaaca cctgtagcat cgatagcagc accaccaatt aaatgatttt cgaaatcgaa 
     7501 cttgacattg gaacgaacat cagaaatagc tttaagaacc ttaatggctt cggctgtgat 
     7561 ttcttgacca acgtggtcac ctggcaaaac gacgatcttc ttaggggcag acattagaat 
     7621 ggtatatcct tgaaatatat atatatattg ctgaaatgta aaaggtaaga aaagttagaa 
     7681 agtaagacga ttgctaacca cctattggaa aaaacaatag gtccttaaat aatattgtca 
     7741 acttcaagta ttgtgatgca agcatttagt catgaacgct tctctattct atatgaaaag 
     7801 ccggttccgg cgctctcacc tttccttttt ctcccaattt ttcagttgaa aaaggtatat 
     7861 gcgtcaggcg acctctgaaa ttaacaaaaa atttccagtc atcgaatttg attctgtgcg 
     7921 atagcgcccc tgtgtgttct cgttatgttg aggaaaaaaa taatggttgc taagagattc 
     7981 gaactcttgc atcttacgat acctgagtat tcccacagtt aattcttgaa gacgaaaggg 
     8041 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc 
     8101 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca 
     8161 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa 
     8221 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt 
     8281 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca 
     8341 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag 
     8401 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc 
     8461 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca 
     8521 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt 
     8581 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct 
     8641 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt 
     8701 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga 
     8761 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact 
     8821 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc 
     8881 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga 
     8941 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt 
     9001 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga 
     9061 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact 
     9121 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga 
     9181 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt 
     9241 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca 
     9301 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct 
     9361 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta 
     9421 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct 
     9481 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc 
     9541 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca 
     9601 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga 
     9661 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg 
     9721 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt 
     9781 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag 
     9841 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt 
     9901 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt 
     9961 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga 
    10021 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta 
    10081 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa 
    10141 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat 
    10201 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta 
    10261 cgccaagctc gaaattaacc ctcactaaag ggaacaaaag ctgggtacc 
//
